Construct: sgRNA BRDN0001145581
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCCTCGCAACAGAAGTAGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACTR2 (10097)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76956
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
65255547 |
- |
ACTR2 |
NNNCTCGCAACAGAAGTAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
16543885 |
+ |
ATXN1 |
NNNCTCGCAACAAAAGTAAC |
NGG |
2 |
0.6593 |
Tier II |
| 3 |
NC_000022.11 |
22 |
24124833 |
- |
CABIN1 |
NNNCTTGCAGCAGAAGTAGC |
NGG |
2 |
0.5159 |
Tier II |
| 4 |
NC_000007.14 |
7 |
141206689 |
- |
TMEM178B |
NNNCTGGCAACAGAAGTAGC |
NGG |
1 |
0.5 |
Tier II |
| 5 |
NC_000019.10 |
19 |
20258821 |
- |
LOC284441 |
NNNCTTGCAACAGAAGTAGC |
NTG |
2 |
0.0362 |
Tier III |
| 6 |
NC_000019.10 |
19 |
20258821 |
- |
LOC105372310 |
NNNCTTGCAACAGAAGTAGC |
NTG |
2 |
0.0362 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
18621308 |
+ |
Gp1bb |
NNNCTAGCAACAGAAGCAGC |
NGG |
2 |
0.6555 |
Tier I |
| 2 |
NC_000077.6 |
11 |
20080171 |
+ |
Actr2 |
NNNCTCGCAACAGCAGCAGC |
NGG |
2 |
0.1513 |
Tier I |
| 3 |
NC_000072.6 |
6 |
90679678 |
+ |
Iqsec1 |
NNNCTCACAACAGAAGCAGC |
NGG |
2 |
0.7059 |
Tier II |
| 4 |
NC_000071.6 |
5 |
13356552 |
- |
Sema3a |
NNNCTAGCAACAGAAGCAGC |
NGG |
2 |
0.6555 |
Tier II |
| 5 |
NC_000071.6 |
5 |
139722664 |
+ |
Micall2 |
NNNCTCCCAACAGAAGTAGA |
NGG |
2 |
0.3438 |
Tier II |
| 6 |
NC_000068.7 |
2 |
131134933 |
- |
Hspa12b |
NNNCTGGCAACAGAAGTGGC |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000074.6 |
8 |
46553597 |
- |
Cenpu |
NNNCTCGAAACAGAAGTAGG |
NGG |
2 |
0.0382 |
Tier II |
Other clones with same target sequence:
(none)
