Construct: sgRNA BRDN0001145583

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGACCCAAACAAAGACATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRPK2 (6733)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75600

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 105167428 - SRPK2 NNNCCCAAACAAAGACATGG NGG 0 1.0 Tier I
2 NC_000005.10 5 111735450 - NREP NNNTCCAAACAAGGACATGG NGG 2 0.5217 Tier I
3 NC_000015.10 15 73322600 + HCN4 NNNCCCAAACAAAGACAGAG NGG 2 0.2381 Tier I
4 NC_000009.12 9 121331387 - GSN NNNCCCAGACAAAGACCTGG NGG 2 0.1294 Tier I
5 NC_000004.12 4 70466556 + MUC7 NNNTCAAAACAAAGACATGG NGG 2 0.7429 Tier II
6 NC_000011.10 11 35329102 + SLC1A2 NNNTCCAAAAAAAGACATGG NGG 2 0.6933 Tier II
7 NC_000002.12 2 232311157 + DIS3L2 NNNCCCAAACAAGGAAATGG NGG 2 0.6522 Tier II
8 NC_000003.12 3 58059854 - FLNB NNNCCCAAACAAGGACATGA NGG 2 0.6114 Tier II
9 NC_000002.12 2 235587136 + AGAP1 NNNCCCAAACAGAAACATGG NGG 2 0.5417 Tier II
10 NC_000007.14 7 37288940 - ELMO1 NNNCCCCAAAAAAGACATGG NGG 2 0.3792 Tier II
11 NC_000001.11 1 78530576 - PTGFR NNNGCCAAACAAAAACATGG NGG 2 0.375 Tier II
12 NC_000010.11 10 7192618 + SFMBT2 NNNCCCAAACAAAAACACGG NGG 2 0.3214 Tier II
13 NC_000010.11 10 26532814 - APBB1IP NNNCCTAAACAAAGACAGGG NGG 2 0.3095 Tier II
14 NC_000007.14 7 28021623 + JAZF1 NNNGACAAACAAAGACATGG NGG 2 0.2857 Tier II
15 NC_000019.10 19 11016523 - SMARCA4 NNNCCCAAACAAGCACATGG NGG 2 0.2795 Tier II
16 NC_000009.12 9 116836710 - ASTN2 NNNCCCAAGGAAAGACATGG NGG 2 0.2593 Tier II
17 NC_000001.11 1 14192129 + KAZN NNNCCCAAACATAGACATAG NGG 2 0.2381 Tier II
18 NC_000009.12 9 34630918 - ARID3C NNNACCAAACAAAGCCATGG NGG 2 0.2297 Tier II
19 NC_000002.12 2 154171171 + GALNT13 NNNACCAAACAAATACATGG NGG 2 0.2246 Tier II
20 NC_000003.12 3 10677262 + ATP2B2 NNNGCCATACAAAGACATGG NGG 2 0.2143 Tier II
21 NC_000013.11 13 26554703 - WASF3 NNNGCCATACAAAGACATGG NGG 2 0.2143 Tier II
22 NC_000009.12 9 70716828 + TRPM3 NNNCCCAAACAAATACATTG NGG 2 0.1778 Tier II
23 NC_000009.12 9 70734821 + TRPM3 NNNCCCAAACAAATACATTG NGG 2 0.1778 Tier II
24 NC_000001.11 1 10274615 + KIF1B NNNCCCAACCAATGACATGG NGG 2 0.1714 Tier II
25 NC_000010.11 10 124525680 - LHPP NNNCCCAACCAAAGCCATGG NGG 2 0.1558 Tier II
26 NC_000020.11 20 47116814 + EYA2 NNNCCCAAACTAAGACATCG NGG 2 0.1379 Tier II
27 NC_000001.11 1 228695733 - RHOU NNNGCCAAACAAATACATGG NGG 2 0.1333 Tier II
28 NC_000006.12 6 44296856 - TCTE1 NNNCCCAAAGTAAGACATGG NGG 2 0.1197 Tier II
29 NC_000007.14 7 42253869 - GLI3 NNNCCCAAAAAAAGACTTGG NGG 2 0.1156 Tier II
30 NC_000008.11 8 62863625 - NKAIN3 NNNCCCAAACAAAGACATGG NGA 1 0.0694 Tier II
31 NC_000021.9 21 41759003 + RIPK4 NNNCCCAAACACAGACATGG NAG 2 0.0682 Tier II
32 NC_000002.12 2 119514130 - SCTR NNNCCCAAAGAAAGAGATGG NGG 2 0.0598 Tier II
33 NC_000012.12 12 57104920 - STAT6 NNNCCCAAACAGAGACATGG NGA 2 0.0502 Tier II
34 NC_000001.11 1 216666575 - ESRRG NNNCCCAAACAAAGACATGA NTG 2 0.0365 Tier II
35 NC_000011.10 11 35397932 + SLC1A2 NNNCCCAAACAAAGACATGA NTG 2 0.0365 Tier II
36 NC_000002.12 2 44164209 - PPM1B-DT NNNCCCAAAAAAAGGCATGG NGG 2 0.5633 Tier III
37 NC_000005.10 5 111735450 - STARD4-AS1 NNNTCCAAACAAGGACATGG NGG 2 0.5217 Tier III
38 NC_000007.14 7 23480814 + LOC389473 NNNTCCAAACAAGGACATGG NGG 2 0.5217 Tier III
39 NC_000008.11 8 53712754 + LOC100129667 NNNTCCAAACAAGGACATGG NGG 2 0.5217 Tier III
40 NC_000016.10 16 17880141 - LOC107984893 NNNCCCAAACAGAGACAAGG NGG 2 0.4815 Tier III
41 NC_000006.12 6 131353951 - LOC105378005 NNNCCCAAACCAAGACATAG NGG 2 0.2857 Tier III
42 NC_000009.12 9 70716828 + LOC105376078 NNNCCCAAACAAATACATTG NGG 2 0.1778 Tier III
43 NC_000002.12 2 200752100 - AOX2P NNNTCCAAACAAAGAGATGG NGG 2 0.1231 Tier III
44 NC_000002.12 2 200752100 - AOX3P-AOX2P NNNTCCAAACAAAGAGATGG NGG 2 0.1231 Tier III
45 NC_000016.10 16 72690145 + ZFHX3-AS1 NNNCCCAAACACAGACATGC NGG 2 0.1128 Tier III
46 NC_000008.11 8 62863625 - SRPK2P NNNCCCAAACAAAGACATGG NGA 1 0.0694 Tier III
47 NC_000008.11 8 62863625 - LOC112268019 NNNCCCAAACAAAGACATGG NGA 1 0.0694 Tier III
48 NC_000006.12 6 8928756 + LOC112267952 NNNCCCAAACACAGACATGG NAG 2 0.0682 Tier III
49 NC_000018.10 18 64212021 + LINC01924 NNNCCCAAACAAAGTTATGG NGG 2 0.0615 Tier III
50 NC_000002.12 2 214909492 - SNHG31 NNNCCCAAAGAAAGACATGG NGA 2 0.027 Tier III
51 NC_000022.11 22 48357036 + LOC105373081 NNNCCCAAACAAAGAGATGG NTG 2 0.006 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 23545574 - Srpk2 NNNCCCAAACAAAGACATGG NAG 1 0.2593 Tier I
2 NC_000081.6 15 76124894 + BC024139 NNNCCAAGACAAAGACATGG NGG 2 0.681 Tier II
3 NC_000073.6 7 134800112 - Dock1 NNNCCCAAATAAAGACATTG NGG 2 0.6275 Tier II
4 NC_000072.6 6 87656402 - Aplf NNNACCAAACAGAGACATGG NGG 2 0.6082 Tier II
5 NC_000077.6 11 81816497 + Asic2 NNNCCCAAAAGAAGACATGG NGG 2 0.5633 Tier II
6 NC_000076.6 10 3979735 + Mthfd1l NNNACCAAGCAAAGACATGG NGG 2 0.5614 Tier II
7 NC_000071.6 5 93520337 + Pramel33 NNNACCAAACGAAGACATGG NGG 2 0.5474 Tier II
8 NC_000071.6 5 93817109 - Pramel35 NNNACCAAACGAAGACATGG NGG 2 0.5474 Tier II
9 NC_000071.6 5 94176416 - Pramel38 NNNACCAAACGAAGACATGG NGG 2 0.5474 Tier II
10 NC_000085.6 19 12606146 + Gm4952 NNNACCAAACGAAGACATGG NGG 2 0.5474 Tier II
11 NT_187056.1 5 85217 + LOC100862115 NNNACCAAACGAAGACATGG NGG 2 0.5474 Tier II
12 NT_187057.1 5 152138 + LOC100862202 NNNACCAAACGAAGACATGG NGG 2 0.5474 Tier II
13 NC_000070.6 4 104097779 - Dab1 NNNACCAACCAAAGACATGG NGG 2 0.4812 Tier II
14 NC_000073.6 7 118927471 + Iqck NNNCTCAAACAAGGACATGG NGG 2 0.415 Tier II
15 NC_000067.6 1 153128190 + Lamc2 NNNCACGAACAAAGACATGG NGG 2 0.4034 Tier II
16 NC_000078.6 12 41026932 - Immp2l NNNCCCAAATAAAGACATGC NGG 2 0.4034 Tier II
17 NC_000075.6 9 72560276 - Rfx7 NNNCCCACAAAAAGACATGG NGG 2 0.3714 Tier II
18 NC_000076.6 10 13153770 + Zc2hc1b NNNCCAAAAGAAAGACATGG NGG 2 0.3611 Tier II
19 NC_000074.6 8 106727337 - Tango6 NNNACCACACAAAGACATGG NGG 2 0.3609 Tier II
20 NC_000071.6 5 95472267 - Pramel48 NNNACCAAACCAAGACATGG NGG 2 0.3368 Tier II
21 NC_000071.6 5 95791366 - E330014E10Rik NNNACCAAACCAAGACATGG NGG 2 0.3368 Tier II
22 NT_166438.1 5 95432 + LOC665290 NNNACCAAACCAAGACATGG NGG 2 0.3368 Tier II
23 NC_000075.6 9 64469476 + Megf11 NNNCTGAAACAAAGACATGG NGG 2 0.3182 Tier II
24 NC_000077.6 11 113954722 - Sdk2 NNNCCCAGACAAAGACATGC NGG 2 0.3143 Tier II
25 NC_000073.6 7 64973080 + Fam189a1 NNNCCCAAATAATGACATGG NGG 2 0.2824 Tier II
26 NC_000081.6 15 102155728 + Soat2 NNNCCCAAATAATGACATGG NGG 2 0.2824 Tier II
27 NC_000078.6 12 8928889 + Laptm4a NNNTCCAAACAATGACATGG NGG 2 0.24 Tier II
28 NC_000071.6 5 123421168 - Mlxip NNNCTCAAACTAAGACATGG NGG 2 0.1958 Tier II
29 NC_000073.6 7 44443842 + Lrrc4b NNNCCCAGACACAGACATGG NGG 2 0.193 Tier II
30 NC_000081.6 15 32522685 - Sema5a NNNCACAAACAAAGACAGGG NGG 2 0.1905 Tier II
31 NC_000077.6 11 97538126 + Srcin1 NNNCCCAAATAAAGACCTGG NGG 2 0.1661 Tier II
32 NC_000068.7 2 103061786 + Pdhx NNNCCCAAACAAAGATATGC NGG 2 0.1319 Tier II
33 NC_000072.6 6 92449460 + Prickle2 NNNCCAAAACAAAGACTTGG NGG 2 0.1238 Tier II
34 NC_000076.6 10 8375641 + Ust NNNCCAAAACAAAGACTTGG NGG 2 0.1238 Tier II
35 NC_000082.6 16 95559751 + Erg NNNCCCAAAGAATGACATGG NGG 2 0.1167 Tier II
36 NC_000069.6 3 90141204 + Nup210l NNNCCCAAAGACAGACATGG NGG 2 0.1023 Tier II
37 NC_000080.6 14 27903628 + Erc2 NNNCCCAAACTAAGCCATGG NGG 2 0.0839 Tier II
38 NC_000069.6 3 146486191 - Spata1 NNNCCCAAATAAAGACATGG NGA 2 0.0654 Tier II
39 NC_000073.6 7 128737831 + Mcmbp NNNCCCAAACAACTACATGG NGG 2 0.0561 Tier II
40 NC_000079.6 13 4604866 - Akr1e1 NNNCCAAAACAAAGACATGG NTG 2 0.0362 Tier II
41 NC_000071.6 5 67403408 - Bend4 NNNCCCACACAAAGACATGG NGA 2 0.0298 Tier II
42 NC_000072.6 6 64453920 - Grid2 NNNCCCAAACAAAGACATAG NTG 2 0.0278 Tier II
43 NC_000084.6 18 75153851 - Dym NNNCCCAAACTAAGACATGG NGA 2 0.0214 Tier II
44 NC_000079.6 13 10144185 + Chrm3 NNNCCCATACAAAGACATGG NTG 2 0.0167 Tier II
45 NC_000068.7 2 167053737 - Znfx1 NNNCCCAAACAAAGACATTG NGC 2 0.0148 Tier II
46 NC_000074.6 8 60649719 + Mfap3l NNNCCCAAACAAACACATGG NGC 2 0.0095 Tier II
47 NC_000080.6 14 77421197 - Enox1 NNNCCCAAACTAAGACATGG NGC 2 0.0068 Tier II
48 NC_000078.6 12 100332815 + Ttc7b NNNCCCAAACAAAGACATGG NTA 2 0.0 Tier II
49 NC_000073.6 7 7755783 + Vmn2r-ps39 NNNACCAAACAGAGACATGG NGG 2 0.6082 Tier III
50 NC_000073.6 7 8555369 + Vmn2r-ps48 NNNACCAAACAGAGACATGG NGG 2 0.6082 Tier III
51 NC_000085.6 19 3178239 - 1700030N03Rik NNNACCAAACAGAGACATGG NGG 2 0.6082 Tier III
52 NC_000073.6 7 67848390 - Gm39027 NNNCCCAAACAAAAACAAGG NGG 2 0.5 Tier III
53 NC_000083.6 17 13324088 - Gm36589 NNNACCAAACAAAGACAGGG NGG 2 0.2807 Tier III
54 NC_000083.6 17 13324088 - Gm36684 NNNACCAAACAAAGACAGGG NGG 2 0.2807 Tier III
55 NC_000069.6 3 143708523 + Gm6260 NNNCCCAAACAAAGACATGA NAG 2 0.2431 Tier III
56 NC_000076.6 10 97722668 + 4930525C09Rik NNNCCCAAACAAAACCATGG NGG 2 0.2045 Tier III
57 NC_000076.6 10 97722668 + Gm10754 NNNCCCAAACAAAACCATGG NGG 2 0.2045 Tier III
58 NC_000069.6 3 52740595 + Gm2447 NNNCCCAAACAAAGACTTGA NGG 2 0.125 Tier III
59 NC_000074.6 8 95901063 - Gm51586 NNNCCCAAACTAAGACATGG NAG 2 0.0798 Tier III
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Other clones with same target sequence:

(none)