Construct: sgRNA BRDN0001145583
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACCCAAACAAAGACATGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- SRPK2 (6733)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000007.14 | 7 | 105167428 | - | SRPK2 | NNNCCCAAACAAAGACATGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000005.10 | 5 | 111735450 | - | NREP | NNNTCCAAACAAGGACATGG | NGG | 2 | 0.5217 | Tier I |
3 | NC_000015.10 | 15 | 73322600 | + | HCN4 | NNNCCCAAACAAAGACAGAG | NGG | 2 | 0.2381 | Tier I |
4 | NC_000009.12 | 9 | 121331387 | - | GSN | NNNCCCAGACAAAGACCTGG | NGG | 2 | 0.1294 | Tier I |
5 | NC_000004.12 | 4 | 70466556 | + | MUC7 | NNNTCAAAACAAAGACATGG | NGG | 2 | 0.7429 | Tier II |
6 | NC_000011.10 | 11 | 35329102 | + | SLC1A2 | NNNTCCAAAAAAAGACATGG | NGG | 2 | 0.6933 | Tier II |
7 | NC_000002.12 | 2 | 232311157 | + | DIS3L2 | NNNCCCAAACAAGGAAATGG | NGG | 2 | 0.6522 | Tier II |
8 | NC_000003.12 | 3 | 58059854 | - | FLNB | NNNCCCAAACAAGGACATGA | NGG | 2 | 0.6114 | Tier II |
9 | NC_000002.12 | 2 | 235587136 | + | AGAP1 | NNNCCCAAACAGAAACATGG | NGG | 2 | 0.5417 | Tier II |
10 | NC_000007.14 | 7 | 37288940 | - | ELMO1 | NNNCCCCAAAAAAGACATGG | NGG | 2 | 0.3792 | Tier II |
11 | NC_000001.11 | 1 | 78530576 | - | PTGFR | NNNGCCAAACAAAAACATGG | NGG | 2 | 0.375 | Tier II |
12 | NC_000010.11 | 10 | 7192618 | + | SFMBT2 | NNNCCCAAACAAAAACACGG | NGG | 2 | 0.3214 | Tier II |
13 | NC_000010.11 | 10 | 26532814 | - | APBB1IP | NNNCCTAAACAAAGACAGGG | NGG | 2 | 0.3095 | Tier II |
14 | NC_000007.14 | 7 | 28021623 | + | JAZF1 | NNNGACAAACAAAGACATGG | NGG | 2 | 0.2857 | Tier II |
15 | NC_000019.10 | 19 | 11016523 | - | SMARCA4 | NNNCCCAAACAAGCACATGG | NGG | 2 | 0.2795 | Tier II |
16 | NC_000009.12 | 9 | 116836710 | - | ASTN2 | NNNCCCAAGGAAAGACATGG | NGG | 2 | 0.2593 | Tier II |
17 | NC_000001.11 | 1 | 14192129 | + | KAZN | NNNCCCAAACATAGACATAG | NGG | 2 | 0.2381 | Tier II |
18 | NC_000009.12 | 9 | 34630918 | - | ARID3C | NNNACCAAACAAAGCCATGG | NGG | 2 | 0.2297 | Tier II |
19 | NC_000002.12 | 2 | 154171171 | + | GALNT13 | NNNACCAAACAAATACATGG | NGG | 2 | 0.2246 | Tier II |
20 | NC_000003.12 | 3 | 10677262 | + | ATP2B2 | NNNGCCATACAAAGACATGG | NGG | 2 | 0.2143 | Tier II |
21 | NC_000013.11 | 13 | 26554703 | - | WASF3 | NNNGCCATACAAAGACATGG | NGG | 2 | 0.2143 | Tier II |
22 | NC_000009.12 | 9 | 70716828 | + | TRPM3 | NNNCCCAAACAAATACATTG | NGG | 2 | 0.1778 | Tier II |
23 | NC_000009.12 | 9 | 70734821 | + | TRPM3 | NNNCCCAAACAAATACATTG | NGG | 2 | 0.1778 | Tier II |
24 | NC_000001.11 | 1 | 10274615 | + | KIF1B | NNNCCCAACCAATGACATGG | NGG | 2 | 0.1714 | Tier II |
25 | NC_000010.11 | 10 | 124525680 | - | LHPP | NNNCCCAACCAAAGCCATGG | NGG | 2 | 0.1558 | Tier II |
26 | NC_000020.11 | 20 | 47116814 | + | EYA2 | NNNCCCAAACTAAGACATCG | NGG | 2 | 0.1379 | Tier II |
27 | NC_000001.11 | 1 | 228695733 | - | RHOU | NNNGCCAAACAAATACATGG | NGG | 2 | 0.1333 | Tier II |
28 | NC_000006.12 | 6 | 44296856 | - | TCTE1 | NNNCCCAAAGTAAGACATGG | NGG | 2 | 0.1197 | Tier II |
29 | NC_000007.14 | 7 | 42253869 | - | GLI3 | NNNCCCAAAAAAAGACTTGG | NGG | 2 | 0.1156 | Tier II |
30 | NC_000008.11 | 8 | 62863625 | - | NKAIN3 | NNNCCCAAACAAAGACATGG | NGA | 1 | 0.0694 | Tier II |
31 | NC_000021.9 | 21 | 41759003 | + | RIPK4 | NNNCCCAAACACAGACATGG | NAG | 2 | 0.0682 | Tier II |
32 | NC_000002.12 | 2 | 119514130 | - | SCTR | NNNCCCAAAGAAAGAGATGG | NGG | 2 | 0.0598 | Tier II |
33 | NC_000012.12 | 12 | 57104920 | - | STAT6 | NNNCCCAAACAGAGACATGG | NGA | 2 | 0.0502 | Tier II |
34 | NC_000001.11 | 1 | 216666575 | - | ESRRG | NNNCCCAAACAAAGACATGA | NTG | 2 | 0.0365 | Tier II |
35 | NC_000011.10 | 11 | 35397932 | + | SLC1A2 | NNNCCCAAACAAAGACATGA | NTG | 2 | 0.0365 | Tier II |
36 | NC_000002.12 | 2 | 44164209 | - | PPM1B-DT | NNNCCCAAAAAAAGGCATGG | NGG | 2 | 0.5633 | Tier III |
37 | NC_000005.10 | 5 | 111735450 | - | STARD4-AS1 | NNNTCCAAACAAGGACATGG | NGG | 2 | 0.5217 | Tier III |
38 | NC_000007.14 | 7 | 23480814 | + | LOC389473 | NNNTCCAAACAAGGACATGG | NGG | 2 | 0.5217 | Tier III |
39 | NC_000008.11 | 8 | 53712754 | + | LOC100129667 | NNNTCCAAACAAGGACATGG | NGG | 2 | 0.5217 | Tier III |
40 | NC_000016.10 | 16 | 17880141 | - | LOC107984893 | NNNCCCAAACAGAGACAAGG | NGG | 2 | 0.4815 | Tier III |
41 | NC_000006.12 | 6 | 131353951 | - | LOC105378005 | NNNCCCAAACCAAGACATAG | NGG | 2 | 0.2857 | Tier III |
42 | NC_000009.12 | 9 | 70716828 | + | LOC105376078 | NNNCCCAAACAAATACATTG | NGG | 2 | 0.1778 | Tier III |
43 | NC_000002.12 | 2 | 200752100 | - | AOX2P | NNNTCCAAACAAAGAGATGG | NGG | 2 | 0.1231 | Tier III |
44 | NC_000002.12 | 2 | 200752100 | - | AOX3P-AOX2P | NNNTCCAAACAAAGAGATGG | NGG | 2 | 0.1231 | Tier III |
45 | NC_000016.10 | 16 | 72690145 | + | ZFHX3-AS1 | NNNCCCAAACACAGACATGC | NGG | 2 | 0.1128 | Tier III |
46 | NC_000008.11 | 8 | 62863625 | - | SRPK2P | NNNCCCAAACAAAGACATGG | NGA | 1 | 0.0694 | Tier III |
47 | NC_000008.11 | 8 | 62863625 | - | LOC112268019 | NNNCCCAAACAAAGACATGG | NGA | 1 | 0.0694 | Tier III |
48 | NC_000006.12 | 6 | 8928756 | + | LOC112267952 | NNNCCCAAACACAGACATGG | NAG | 2 | 0.0682 | Tier III |
49 | NC_000018.10 | 18 | 64212021 | + | LINC01924 | NNNCCCAAACAAAGTTATGG | NGG | 2 | 0.0615 | Tier III |
50 | NC_000002.12 | 2 | 214909492 | - | SNHG31 | NNNCCCAAAGAAAGACATGG | NGA | 2 | 0.027 | Tier III |
51 | NC_000022.11 | 22 | 48357036 | + | LOC105373081 | NNNCCCAAACAAAGAGATGG | NTG | 2 | 0.006 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000071.6 | 5 | 23545574 | - | Srpk2 | NNNCCCAAACAAAGACATGG | NAG | 1 | 0.2593 | Tier I |
2 | NC_000081.6 | 15 | 76124894 | + | BC024139 | NNNCCAAGACAAAGACATGG | NGG | 2 | 0.681 | Tier II |
3 | NC_000073.6 | 7 | 134800112 | - | Dock1 | NNNCCCAAATAAAGACATTG | NGG | 2 | 0.6275 | Tier II |
4 | NC_000072.6 | 6 | 87656402 | - | Aplf | NNNACCAAACAGAGACATGG | NGG | 2 | 0.6082 | Tier II |
5 | NC_000077.6 | 11 | 81816497 | + | Asic2 | NNNCCCAAAAGAAGACATGG | NGG | 2 | 0.5633 | Tier II |
6 | NC_000076.6 | 10 | 3979735 | + | Mthfd1l | NNNACCAAGCAAAGACATGG | NGG | 2 | 0.5614 | Tier II |
7 | NC_000071.6 | 5 | 93520337 | + | Pramel33 | NNNACCAAACGAAGACATGG | NGG | 2 | 0.5474 | Tier II |
8 | NC_000071.6 | 5 | 93817109 | - | Pramel35 | NNNACCAAACGAAGACATGG | NGG | 2 | 0.5474 | Tier II |
9 | NC_000071.6 | 5 | 94176416 | - | Pramel38 | NNNACCAAACGAAGACATGG | NGG | 2 | 0.5474 | Tier II |
10 | NC_000085.6 | 19 | 12606146 | + | Gm4952 | NNNACCAAACGAAGACATGG | NGG | 2 | 0.5474 | Tier II |
11 | NT_187056.1 | 5 | 85217 | + | LOC100862115 | NNNACCAAACGAAGACATGG | NGG | 2 | 0.5474 | Tier II |
12 | NT_187057.1 | 5 | 152138 | + | LOC100862202 | NNNACCAAACGAAGACATGG | NGG | 2 | 0.5474 | Tier II |
13 | NC_000070.6 | 4 | 104097779 | - | Dab1 | NNNACCAACCAAAGACATGG | NGG | 2 | 0.4812 | Tier II |
14 | NC_000073.6 | 7 | 118927471 | + | Iqck | NNNCTCAAACAAGGACATGG | NGG | 2 | 0.415 | Tier II |
15 | NC_000067.6 | 1 | 153128190 | + | Lamc2 | NNNCACGAACAAAGACATGG | NGG | 2 | 0.4034 | Tier II |
16 | NC_000078.6 | 12 | 41026932 | - | Immp2l | NNNCCCAAATAAAGACATGC | NGG | 2 | 0.4034 | Tier II |
17 | NC_000075.6 | 9 | 72560276 | - | Rfx7 | NNNCCCACAAAAAGACATGG | NGG | 2 | 0.3714 | Tier II |
18 | NC_000076.6 | 10 | 13153770 | + | Zc2hc1b | NNNCCAAAAGAAAGACATGG | NGG | 2 | 0.3611 | Tier II |
19 | NC_000074.6 | 8 | 106727337 | - | Tango6 | NNNACCACACAAAGACATGG | NGG | 2 | 0.3609 | Tier II |
20 | NC_000071.6 | 5 | 95472267 | - | Pramel48 | NNNACCAAACCAAGACATGG | NGG | 2 | 0.3368 | Tier II |
21 | NC_000071.6 | 5 | 95791366 | - | E330014E10Rik | NNNACCAAACCAAGACATGG | NGG | 2 | 0.3368 | Tier II |
22 | NT_166438.1 | 5 | 95432 | + | LOC665290 | NNNACCAAACCAAGACATGG | NGG | 2 | 0.3368 | Tier II |
23 | NC_000075.6 | 9 | 64469476 | + | Megf11 | NNNCTGAAACAAAGACATGG | NGG | 2 | 0.3182 | Tier II |
24 | NC_000077.6 | 11 | 113954722 | - | Sdk2 | NNNCCCAGACAAAGACATGC | NGG | 2 | 0.3143 | Tier II |
25 | NC_000073.6 | 7 | 64973080 | + | Fam189a1 | NNNCCCAAATAATGACATGG | NGG | 2 | 0.2824 | Tier II |
26 | NC_000081.6 | 15 | 102155728 | + | Soat2 | NNNCCCAAATAATGACATGG | NGG | 2 | 0.2824 | Tier II |
27 | NC_000078.6 | 12 | 8928889 | + | Laptm4a | NNNTCCAAACAATGACATGG | NGG | 2 | 0.24 | Tier II |
28 | NC_000071.6 | 5 | 123421168 | - | Mlxip | NNNCTCAAACTAAGACATGG | NGG | 2 | 0.1958 | Tier II |
29 | NC_000073.6 | 7 | 44443842 | + | Lrrc4b | NNNCCCAGACACAGACATGG | NGG | 2 | 0.193 | Tier II |
30 | NC_000081.6 | 15 | 32522685 | - | Sema5a | NNNCACAAACAAAGACAGGG | NGG | 2 | 0.1905 | Tier II |
31 | NC_000077.6 | 11 | 97538126 | + | Srcin1 | NNNCCCAAATAAAGACCTGG | NGG | 2 | 0.1661 | Tier II |
32 | NC_000068.7 | 2 | 103061786 | + | Pdhx | NNNCCCAAACAAAGATATGC | NGG | 2 | 0.1319 | Tier II |
33 | NC_000072.6 | 6 | 92449460 | + | Prickle2 | NNNCCAAAACAAAGACTTGG | NGG | 2 | 0.1238 | Tier II |
34 | NC_000076.6 | 10 | 8375641 | + | Ust | NNNCCAAAACAAAGACTTGG | NGG | 2 | 0.1238 | Tier II |
35 | NC_000082.6 | 16 | 95559751 | + | Erg | NNNCCCAAAGAATGACATGG | NGG | 2 | 0.1167 | Tier II |
36 | NC_000069.6 | 3 | 90141204 | + | Nup210l | NNNCCCAAAGACAGACATGG | NGG | 2 | 0.1023 | Tier II |
37 | NC_000080.6 | 14 | 27903628 | + | Erc2 | NNNCCCAAACTAAGCCATGG | NGG | 2 | 0.0839 | Tier II |
38 | NC_000069.6 | 3 | 146486191 | - | Spata1 | NNNCCCAAATAAAGACATGG | NGA | 2 | 0.0654 | Tier II |
39 | NC_000073.6 | 7 | 128737831 | + | Mcmbp | NNNCCCAAACAACTACATGG | NGG | 2 | 0.0561 | Tier II |
40 | NC_000079.6 | 13 | 4604866 | - | Akr1e1 | NNNCCAAAACAAAGACATGG | NTG | 2 | 0.0362 | Tier II |
41 | NC_000071.6 | 5 | 67403408 | - | Bend4 | NNNCCCACACAAAGACATGG | NGA | 2 | 0.0298 | Tier II |
42 | NC_000072.6 | 6 | 64453920 | - | Grid2 | NNNCCCAAACAAAGACATAG | NTG | 2 | 0.0278 | Tier II |
43 | NC_000084.6 | 18 | 75153851 | - | Dym | NNNCCCAAACTAAGACATGG | NGA | 2 | 0.0214 | Tier II |
44 | NC_000079.6 | 13 | 10144185 | + | Chrm3 | NNNCCCATACAAAGACATGG | NTG | 2 | 0.0167 | Tier II |
45 | NC_000068.7 | 2 | 167053737 | - | Znfx1 | NNNCCCAAACAAAGACATTG | NGC | 2 | 0.0148 | Tier II |
46 | NC_000074.6 | 8 | 60649719 | + | Mfap3l | NNNCCCAAACAAACACATGG | NGC | 2 | 0.0095 | Tier II |
47 | NC_000080.6 | 14 | 77421197 | - | Enox1 | NNNCCCAAACTAAGACATGG | NGC | 2 | 0.0068 | Tier II |
48 | NC_000078.6 | 12 | 100332815 | + | Ttc7b | NNNCCCAAACAAAGACATGG | NTA | 2 | 0.0 | Tier II |
49 | NC_000073.6 | 7 | 7755783 | + | Vmn2r-ps39 | NNNACCAAACAGAGACATGG | NGG | 2 | 0.6082 | Tier III |
50 | NC_000073.6 | 7 | 8555369 | + | Vmn2r-ps48 | NNNACCAAACAGAGACATGG | NGG | 2 | 0.6082 | Tier III |
51 | NC_000085.6 | 19 | 3178239 | - | 1700030N03Rik | NNNACCAAACAGAGACATGG | NGG | 2 | 0.6082 | Tier III |
52 | NC_000073.6 | 7 | 67848390 | - | Gm39027 | NNNCCCAAACAAAAACAAGG | NGG | 2 | 0.5 | Tier III |
53 | NC_000083.6 | 17 | 13324088 | - | Gm36589 | NNNACCAAACAAAGACAGGG | NGG | 2 | 0.2807 | Tier III |
54 | NC_000083.6 | 17 | 13324088 | - | Gm36684 | NNNACCAAACAAAGACAGGG | NGG | 2 | 0.2807 | Tier III |
55 | NC_000069.6 | 3 | 143708523 | + | Gm6260 | NNNCCCAAACAAAGACATGA | NAG | 2 | 0.2431 | Tier III |
56 | NC_000076.6 | 10 | 97722668 | + | 4930525C09Rik | NNNCCCAAACAAAACCATGG | NGG | 2 | 0.2045 | Tier III |
57 | NC_000076.6 | 10 | 97722668 | + | Gm10754 | NNNCCCAAACAAAACCATGG | NGG | 2 | 0.2045 | Tier III |
58 | NC_000069.6 | 3 | 52740595 | + | Gm2447 | NNNCCCAAACAAAGACTTGA | NGG | 2 | 0.125 | Tier III |
59 | NC_000074.6 | 8 | 95901063 | - | Gm51586 | NNNCCCAAACTAAGACATGG | NAG | 2 | 0.0798 | Tier III |