Construct: sgRNA BRDN0001145587
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATCACCCGTGACTGGAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SCYL3 (57147)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76795
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
169862753 |
+ |
SCYL3 |
NNNCACCCGTGACTGGAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
42730589 |
- |
CDAN1 |
NNNCACCGGTGAGTGGAACA |
NGG |
2 |
0.0877 |
Tier I |
| 3 |
NC_000001.11 |
1 |
2487317 |
- |
PLCH2 |
NNNCACCCGTGACTGGGACA |
NGA |
2 |
0.0123 |
Tier I |
| 4 |
NC_000012.12 |
12 |
111052075 |
- |
CUX2 |
NNNCACGTGTGACTGGAACA |
NGG |
2 |
0.4118 |
Tier II |
| 5 |
NC_000016.10 |
16 |
78675479 |
- |
WWOX |
NNNCAACCGTGCCTGGAACA |
NGG |
2 |
0.2444 |
Tier II |
| 6 |
NC_000008.11 |
8 |
125047125 |
+ |
WASHC5 |
NNNCCCCCGTGACTGGAATA |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000007.14 |
7 |
73603514 |
+ |
MLXIPL |
NNNCTCCCGGGACTGGAACA |
NGG |
2 |
0.1818 |
Tier II |
| 8 |
NC_000016.10 |
16 |
85709023 |
+ |
C16orf74 |
NNNCACCCGTGACTAGCACA |
NGG |
2 |
0.1661 |
Tier II |
| 9 |
NC_000009.12 |
9 |
131393269 |
+ |
PRRC2B |
NNNCAACCGTGACTGGAAGA |
NGG |
2 |
0.1161 |
Tier II |
| 10 |
NC_000001.11 |
1 |
241600826 |
- |
OPN3 |
NNNCACCTGTGACTGGAACA |
NTG |
2 |
0.0341 |
Tier II |
| 11 |
NC_000004.12 |
4 |
123107054 |
+ |
AFG2A |
NNNCACCCGTGACTGGACGA |
NGG |
2 |
0.0238 |
Tier II |
| 12 |
NC_000012.12 |
12 |
122137221 |
+ |
MLXIP |
NNNCACCAGTGACTGGAACA |
NGT |
2 |
0.0105 |
Tier II |
| 13 |
NC_000020.11 |
20 |
32139505 |
- |
TM9SF4 |
NNNCACCGGTGACTGGAACA |
NGT |
2 |
0.0104 |
Tier II |
| 14 |
NC_000012.12 |
12 |
2264093 |
- |
CACNA1C |
NNNCACCCGTGAGTGCAACA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000017.11 |
17 |
58334663 |
+ |
TSPOAP1-AS1 |
NNNCACCTGTGACTGGAACA |
NGG |
1 |
0.875 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
13291869 |
+ |
Mrtfb |
NNNCACCAGGGACTGGAACA |
NGG |
2 |
0.325 |
Tier II |
| 2 |
NC_000070.6 |
4 |
95748135 |
+ |
Fggy |
NNNCACCCGTGTCTTGAACA |
NGG |
2 |
0.0476 |
Tier II |
| 3 |
NC_000072.6 |
6 |
94264571 |
- |
Magi1 |
NNNCACCAGTGACTGGAACA |
NGC |
2 |
0.0144 |
Tier II |
| 4 |
NC_000074.6 |
8 |
36570251 |
- |
Dlc1 |
NNNCACCCGTGACTGCAACC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000070.6 |
4 |
149360968 |
+ |
Ube4b |
NNNCACCTGTGACTGCAACA |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000072.6 |
6 |
55157976 |
+ |
Gm34358 |
NNNCACCGATGACTGGAACA |
NGG |
2 |
0.4133 |
Tier III |
| 7 |
NC_000082.6 |
16 |
92176296 |
- |
Gm41496 |
NNNCCCCAGTGACTGGAACA |
NGG |
2 |
0.325 |
Tier III |
| 8 |
NC_000068.7 |
2 |
78051292 |
+ |
4930440I19Rik |
NNNCACCCGTGACTGGGAAA |
NGG |
2 |
0.0756 |
Tier III |
| 9 |
NC_000068.7 |
2 |
78051292 |
+ |
Gm52519 |
NNNCACCCGTGACTGGGAAA |
NGG |
2 |
0.0756 |
Tier III |
Other clones with same target sequence:
(none)
