Construct: sgRNA BRDN0001145598
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGGGCAGAACGACCCTGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80173
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
56283280 |
+ |
GNAO1 |
NNNGGCAGCACGACCCTAAC |
NGG |
2 |
0.3956 |
Tier II |
| 2 |
NC_000017.11 |
17 |
28906810 |
+ |
PHF12 |
NNNGTCAGAACCACCCTGAC |
NGG |
2 |
0.1588 |
Tier II |
| 3 |
NC_000001.11 |
1 |
84872408 |
- |
LPAR3 |
NNNTTCAGAACGACCCTGAC |
NGG |
2 |
0.1091 |
Tier II |
| 4 |
NC_000006.12 |
6 |
160446215 |
+ |
SLC22A3 |
NNNGGCAGAAGGAACCTGAC |
NGG |
2 |
0.0875 |
Tier II |
| 5 |
NC_000010.11 |
10 |
124542910 |
- |
LHPP |
NNNGGGAGAACGACCGTGAC |
NGG |
2 |
0.0769 |
Tier II |
| 6 |
NC_000004.12 |
4 |
65399446 |
+ |
EPHA5 |
NNNGGCAGAACGAGCCTAAC |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
77406182 |
- |
Epha4 |
NNNGGCAGAAAAACCCTGAC |
NGG |
2 |
0.7 |
Tier II |
| 2 |
NC_000072.6 |
6 |
125903947 |
- |
Ano2 |
NNNAGCAGAAAGACCCTGAC |
NGG |
2 |
0.675 |
Tier II |
| 3 |
NC_000084.6 |
18 |
35976187 |
+ |
Psd2 |
NNNGGCAGAACCACCCTGAC |
NGG |
1 |
0.5294 |
Tier II |
| 4 |
NC_000074.6 |
8 |
91666215 |
- |
Fto |
NNNGGCAGAACGGCCCTGAC |
NAG |
2 |
0.1691 |
Tier II |
| 5 |
NC_000069.6 |
3 |
89764200 |
+ |
Chrnb2 |
NNNTGCAGAACGACCCTGGC |
NGG |
2 |
0.1364 |
Tier II |
| 6 |
NC_000079.6 |
13 |
51394937 |
+ |
Gm32834 |
NNNTGCAGAACGACCCTGAC |
NGA |
2 |
0.0253 |
Tier III |
Other clones with same target sequence:
(none)
