Construct: sgRNA BRDN0001145600

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCCTCAGGAACTCGCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITK (3702)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77448

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 157222941 + ITK NNNCTCAGGAACTCGCACTG NGG 0 1.0 Tier I
2 NC_000017.11 17 74943232 + OTOP3 NNNCCCAGGAACTCACACTG NGG 2 0.9412 Tier II
3 NC_000001.11 1 115762925 + CASQ2 NNNCTCAGGAACTCACAATG NGG 2 0.5068 Tier II
4 NC_000013.11 13 113764384 - TMEM255B NNNCTCAGGAACACGCCCTG NGG 2 0.1222 Tier II
5 NC_000003.12 3 50560115 - C3orf18 NNNTTCAGGAACTCGCACTG NGA 2 0.0556 Tier II
6 NC_000005.10 5 1140637 + SLC12A7 NNNCTCAGGAACTCGCACCG NTG 2 0.0107 Tier II
7 NC_000002.12 2 197163397 - ANKRD44 NNNCCCAGGAACTGGCACTG NGG 2 0.0 Tier II
8 NC_000020.11 20 63318967 + COL20A1 NNNCTCAGGGACTGGCACTG NGG 2 0.0 Tier II
9 NC_000013.11 13 105721692 + LINC00343 NNNCTCAGGAACTTGGACTG NGG 2 0.1128 Tier III
10 NC_000006.12 6 170439604 - LOC107986676 NNNCTCAGGAACTCCCTCTG NGG 2 0.0364 Tier III
11 NC_000010.11 10 101230464 + LBX1-AS1 NNNCTCAGGAACTCGCAGGG NGG 2 0.0333 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 46355799 - Itk NNNCTCAGGAGCTCGCACTG NGG 1 0.65 Tier I
2 NC_000075.6 9 14860577 - Gpr83 NNNCTCAGTAGCTCGCACTG NGG 2 0.3467 Tier I
3 NC_000070.6 4 148439173 + Ubiad1 NNNCTCAAGAACTCACACTG NGG 2 0.9412 Tier II
4 NC_000072.6 6 129874141 + Klra17 NNNTTCAGGAACTCACACTG NGG 2 0.7529 Tier II
5 NC_000078.6 12 57031143 + Slc25a21 NNNCCCAGGAACTTGCACTG NGG 2 0.7333 Tier II
6 NC_000077.6 11 117265321 + Septin9 NNNCTCAGGGACTCACACTG NGG 2 0.5229 Tier II
7 NC_000069.6 3 83987790 + Tmem131l NNNCTTAGGAATTCGCACTG NGG 2 0.5 Tier II
8 NC_000070.6 4 107806276 + Lrp8 NNNCTCTGGAACTCACACTG NGG 2 0.4118 Tier II
9 NC_000071.6 5 137416124 + Zan NNNCTCAGGTCCTCGCACTG NGG 2 0.3529 Tier II
10 NC_000070.6 4 137892959 + Ece1 NNNCTCAGGAACTCGTACTG NGG 1 0.3077 Tier II
11 NC_000068.7 2 5045592 - Optn NNNCTCAGGAACTCACACAG NGG 2 0.2689 Tier II
12 NC_000069.6 3 102483615 - Ngf NNNCCCAGGAACTCGCACTG NAG 2 0.2593 Tier II
13 NC_000077.6 11 74794698 - Mettl16 NNNCTCAGGAACTCACACTG NAG 2 0.244 Tier II
14 NC_000086.7 X 9272968 - Xk NNNCTCAGGGACTCGCACCG NGG 2 0.1533 Tier II
15 NC_000070.6 4 145084591 + Vps13d NNNCTCAGGAACTAGCACTC NGG 2 0.15 Tier II
16 NC_000078.6 12 87368459 - Sptlc2 NNNCTCAGGAACTCACTCTG NGG 2 0.1255 Tier II
17 NC_000082.6 16 94504656 - Vps26c NNNCTCAGGAACTCACTCTG NGG 2 0.1255 Tier II
18 NC_000072.6 6 130230847 + Klra7 NNNTTCAGGAACTCTCACTG NGG 2 0.1143 Tier II
19 NC_000071.6 5 76262226 + Clock NNNCTCAGGAGCTCTCACTG NGG 2 0.0929 Tier II
20 NC_000073.6 7 44459026 - Lrrc4b NNNCTCAGAAACTCTCACTG NGG 2 0.0918 Tier II
21 NC_000072.6 6 82434175 - Tacr1 NNNCTCAGGAACTCCCACAG NGG 2 0.0779 Tier II
22 NC_000069.6 3 122169706 + Abca4 NNNCTCAGGAAGTCGCACTG NTG 2 0.0173 Tier II
23 NC_000073.6 7 101325401 - Stard10 NNNCTCAGGAACTCTCACTG NTG 2 0.0056 Tier II
24 NC_000069.6 3 108426095 - Sars NNNCTCAGGAACTCCCACTG NGT 2 0.0044 Tier II
25 NC_000076.6 10 121518013 + Gm35696 NNNCTCAGGAACCTGCACTG NGG 2 0.5789 Tier III
26 NC_000077.6 11 117265321 + Gm39478 NNNCTCAGGGACTCACACTG NGG 2 0.5229 Tier III
27 NC_000070.6 4 107806276 + Lrp8os2 NNNCTCTGGAACTCACACTG NGG 2 0.4118 Tier III
28 NC_000082.6 16 94504656 - Gm31323 NNNCTCAGGAACTCACTCTG NGG 2 0.1255 Tier III
29 NC_000073.6 7 101325401 - Gm20476 NNNCTCAGGAACTCTCACTG NTG 2 0.0056 Tier III
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Other clones with same target sequence:

(none)