Construct: sgRNA BRDN0001145601
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAAGACTGTAAACCTGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKB (1607)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76916
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
14607472 |
+ |
DGKB |
NNNAGACTGTAAACCTGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
40565706 |
+ |
MUC19 |
NNNAGACTGTAAACCTGAGG |
NGT |
2 |
0.002 |
Tier I |
| 3 |
NC_000007.14 |
7 |
149157056 |
- |
ZNF398 |
NNNAGACAGTAAACCTAACG |
NGG |
2 |
0.7467 |
Tier II |
| 4 |
NC_000018.10 |
18 |
45831525 |
+ |
EPG5 |
NNNAGACTGTCAACCTAACG |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000018.10 |
18 |
45831525 |
+ |
SIGLEC15 |
NNNAGACTGTCAACCTAACG |
NGG |
2 |
0.3733 |
Tier II |
| 6 |
NC_000004.12 |
4 |
8486807 |
+ |
TRMT44 |
NNNAAACTGTAAACCGGACG |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000006.12 |
6 |
36687229 |
+ |
CDKN1A |
NNNAGACTGTAAACCTCTCG |
NGG |
2 |
0.1176 |
Tier II |
| 8 |
NC_000016.10 |
16 |
69841171 |
- |
WWP2 |
NNNAGACTGTAAACCTGAAG |
NAG |
2 |
0.1111 |
Tier II |
| 9 |
NC_000007.14 |
7 |
32316104 |
+ |
PDE1C |
NNNAGACTGTAAACCTCATG |
NGG |
2 |
0.1086 |
Tier II |
| 10 |
NC_000010.11 |
10 |
62907459 |
- |
LOC107984012 |
NNNAGACTATAAACCTGACA |
NGG |
2 |
0.6027 |
Tier III |
| 11 |
NC_000001.11 |
1 |
211208192 |
+ |
PRELID1P5 |
NNNAGACTGTAAGCCCGACG |
NGG |
2 |
0.4348 |
Tier III |
| 12 |
NC_000013.11 |
13 |
53557370 |
- |
LOC105370210 |
NNNAGACTGTCAACCTGACA |
NGG |
2 |
0.375 |
Tier III |
| 13 |
NC_000001.11 |
1 |
223145475 |
+ |
LOC124904522 |
NNNAGACTGTAAGCCTCACG |
NGG |
2 |
0.1535 |
Tier III |
| 14 |
NC_000003.12 |
3 |
67844049 |
+ |
SUCLG2-DT |
NNNAGACTGTAAGCCTGCCG |
NGG |
2 |
0.1242 |
Tier III |
| 15 |
NC_000002.12 |
2 |
170970915 |
+ |
LOC124906091 |
NNNAGACTGTAAACCTGATG |
NAG |
2 |
0.1197 |
Tier III |
| 16 |
NC_000001.11 |
1 |
101066720 |
- |
DPH5-DT |
NNNAGACTGTAAACCTGACA |
NGA |
2 |
0.0651 |
Tier III |
| 17 |
NC_000003.12 |
3 |
72297086 |
- |
LOC105377158 |
NNNAGACTGTAAACTTGACA |
NGG |
2 |
0.0625 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
38190097 |
- |
Dgkb |
NNNAGACTGTAAACCTGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
118396064 |
- |
Cdh13 |
NNNTGACTGTAAACCTGACA |
NGG |
2 |
0.5966 |
Tier II |
| 3 |
NC_000076.6 |
10 |
105423759 |
+ |
Tmtc2 |
NNNAGACTGTAAACCTGTCA |
NGG |
2 |
0.4688 |
Tier II |
| 4 |
NC_000083.6 |
17 |
75217862 |
- |
Ltbp1 |
NNNAGACTCTGAACCTGACG |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000071.6 |
5 |
107119548 |
- |
Tgfbr3 |
NNNTGACTGTAAAACTGACG |
NGG |
2 |
0.2227 |
Tier II |
| 6 |
NC_000074.6 |
8 |
126221590 |
+ |
Slc35f3 |
NNNAGTCTGTAACCCTGACG |
NGG |
2 |
0.1504 |
Tier II |
| 7 |
NC_000074.6 |
8 |
67774290 |
- |
Psd3 |
NNNAGACTGTAAATCTGAGG |
NGG |
2 |
0.0917 |
Tier II |
| 8 |
NC_000082.6 |
16 |
56061955 |
- |
Senp7 |
NNNAGACTGTTAACCTTACG |
NGG |
2 |
0.0769 |
Tier II |
| 9 |
NC_000081.6 |
15 |
35791468 |
+ |
Vps13b |
NNNAGACTGTAAACCTCAGG |
NGG |
2 |
0.0294 |
Tier II |
| 10 |
NC_000077.6 |
11 |
3136314 |
+ |
Sfi1 |
NNNAGACTGTAAACCTGACT |
NTG |
2 |
0.0273 |
Tier II |
| 11 |
NC_000079.6 |
13 |
6576930 |
- |
Pitrm1 |
NNNAGACTGTCAACCTGACG |
NGC |
2 |
0.0089 |
Tier II |
| 12 |
NC_000072.6 |
6 |
123065309 |
- |
Clec4b1 |
NNNAGACTGAAAAGCTGACG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
