Construct: sgRNA BRDN0001145608
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGTTCGCCGGTCACAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK4 (10298)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77575
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
39173138 |
+ |
PAK4 |
NNNGTTCGCCGGTCACAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
24081296 |
+ |
NRL |
NNNGTTAGCCGGTCACAGCG |
NGC |
2 |
0.0167 |
Tier I |
| 3 |
NC_000016.10 |
16 |
1493923 |
- |
TELO2 |
NNNGTTCCCCGGGCACAGCG |
NGG |
2 |
0.1605 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
6100553 |
+ |
Proser2 |
NNNGCTTGCCGGTCACAGCG |
NGG |
2 |
0.8125 |
Tier I |
| 2 |
NC_000085.6 |
19 |
28467104 |
- |
Glis3 |
NNNGTTCTCAGGTCACAGCG |
NGG |
2 |
0.5417 |
Tier II |
| 3 |
NC_000072.6 |
6 |
118893538 |
- |
Cacna1c |
NNNGTTCGCCAGGCACAGCG |
NGG |
2 |
0.2609 |
Tier II |
Other clones with same target sequence:
(none)
