Construct: sgRNA BRDN0001145616

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAGGGCCACACATTGGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IKBKB (3551)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76089

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 42317679 + IKBKB NNNGGGCCACACATTGGACA NGG 0 1.0 Tier I
2 NC_000001.11 1 113110353 - LRIG2 NNNGCGCCACACAGTGGACA NGG 2 0.0 Tier I
3 NC_000001.11 1 11772165 + C1orf167 NNNGGGCCACACAGAGGACA NGG 2 0.0 Tier I
4 NC_000005.10 5 146281655 + RBM27 NNNGGGCCACAGGTTGGACA NGG 2 0.2899 Tier II
5 NC_000005.10 5 146281655 + LOC127814297 NNNGGGCCACAGGTTGGACA NGG 2 0.2899 Tier II
6 NC_000008.11 8 130116070 - ASAP1 NNNGGGCCACAGATTGGACT NGG 2 0.2667 Tier II
7 NC_000005.10 5 150524362 - NDST1 NNNGGGGCACACATTGGTCA NGG 2 0.2353 Tier II
8 NC_000006.12 6 56987430 - BEND6 NNNGGGCCACAGATTGGAAA NGG 2 0.1905 Tier II
9 NC_000019.10 19 46298116 - HIF3A NNNGTGCCTCACATTGGACA NGG 2 0.18 Tier II
10 NC_000003.12 3 9217440 + SRGAP3 NNNGGGCCTCACCTTGGACA NGG 2 0.1263 Tier II
11 NC_000011.10 11 134381891 - B3GAT1 NNNGGGCCACACACTGGGCA NGG 2 0.1143 Tier II
12 NC_000016.10 16 22250854 + EEF2K NNNGGGCCACACATTGGGCC NGG 2 0.0909 Tier II
13 NC_000017.11 17 8436238 - NDEL1 NNNGGGCCACACATCGGACC NGG 2 0.062 Tier II
14 NC_000023.11 X 18907308 - PHKA2 NNNGGGCCACACATTGGAGA NAG 2 0.0324 Tier II
15 NC_000001.11 1 14610072 - KAZN NNNGGGCCACTCATTGGACA NGA 2 0.0214 Tier II
16 NC_000016.10 16 68356467 - PRMT7 NNNGGGCCACACATGGGGCA NGG 2 0.02 Tier II
17 NC_000012.12 12 112508746 - PTPN11 NNNGGGCCACACACTGGACA NGA 2 0.0198 Tier II
18 NC_000023.11 X 105019968 - IL1RAPL2 NNNGGGCCACACATTGCACA NGA 2 0.0163 Tier II
19 NC_000017.11 17 80827615 + RPTOR NNNGGGCCACACCTGGGACA NGG 2 0.0105 Tier II
20 NC_000001.11 1 152324589 - FLG NNNGGGCCACAGATTGGACA NGC 2 0.0099 Tier II
21 NC_000001.11 1 205095281 - RBBP5 NNNGGGCCACAGATTGGACA NGC 2 0.0099 Tier II
22 NC_000003.12 3 67564603 - SUCLG2 NNNGGGCCACAGATTGGACA NGC 2 0.0099 Tier II
23 NC_000022.11 22 38142726 + PLA2G6 NNNGGGCCACAGATTGGACA NGC 2 0.0099 Tier II
24 NC_000004.12 4 56964754 + NOA1 NNNGGGCCACACATTGGGCA NGC 2 0.0089 Tier II
25 NC_000003.12 3 160991194 + PPM1L NNNGGGCCACAGATTGGACA NGT 2 0.0072 Tier II
26 NC_000020.11 20 38305688 - BPI NNNGGGCCACACAGCGGACA NGG 2 0.0 Tier II
27 NC_000020.11 20 33008279 - BPIFB2 NNNGGGCCACACAGTGGACT NGG 2 0.0 Tier II
28 NC_000003.12 3 14507380 - GRIP2 NNNGGGCCACACAGTGGGCA NGG 2 0.0 Tier II
29 NC_000003.12 3 54916387 - CACNA2D3 NNNGGGCCACTCAGTGGACA NGG 2 0.0 Tier II
30 NC_000003.12 3 9217440 + SRGAP3-AS3 NNNGGGCCTCACCTTGGACA NGG 2 0.1263 Tier III
31 NC_000002.12 2 47225902 - EPCAM-DT NNNGGGCCACACATTGGCCT NGG 2 0.1143 Tier III
32 NC_000003.12 3 150734633 + LOC101928105 NNNGGGCCACACTCTGGACA NGG 2 0.0857 Tier III
33 NC_000004.12 4 121983718 + LOC102724158 NNNGGGCCACACATTGGACA NGC 1 0.0222 Tier III
34 NC_000001.11 1 152324589 - CCDST NNNGGGCCACAGATTGGACA NGC 2 0.0099 Tier III
35 NC_000006.12 6 22412680 - LOC105374971 NNNGGGCCACAGATTGGACA NGC 2 0.0099 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 22671724 - Ikbkb NNNGGGCCTCACGTTGGACA NGG 2 0.3913 Tier I
2 NC_000069.6 3 53537309 + Frem2 NNNGGGCCGCACATTGGACC NGG 2 0.1515 Tier I
3 NC_000072.6 6 115007865 - Tamm41 NNNGGGCCCCACATTGTACA NGG 2 0.1429 Tier II
4 NC_000081.6 15 88892404 - Ttll8 NNNGGGCCACACATGGGACA NGG 1 0.05 Tier II
5 NC_000074.6 8 124938936 - Egln1 NNNGGGCCACAGATTGGACA NGT 2 0.0072 Tier II
6 NC_000079.6 13 24236843 + Carmil1 NNNGGACCACACAGTGGACA NGG 2 0.0 Tier II
7 NC_000071.6 5 30413657 + Otof NNNGGGCAACACAGTGGACA NGG 2 0.0 Tier II
8 NC_000077.6 11 54102639 - P4ha2 NNNGGGCCACACAGTGGACA NGC 2 0.0 Tier II
9 NC_000073.6 7 37613771 + Zfp536 NNNGGGCCACACAGTGGCCA NGG 2 0.0 Tier II
10 NC_000073.6 7 68350110 + Fam169b NNNGGGCCACACATTTGACC NGG 2 0.0 Tier II
11 NC_000075.6 9 44072077 + Usp2 NNNGGGCCCCACAGTGGACA NGG 2 0.0 Tier II
12 NC_000081.6 15 88892404 - Gm38643 NNNGGGCCACACATGGGACA NGG 1 0.05 Tier III
13 NC_000074.6 8 124938936 - Gm46060 NNNGGGCCACAGATTGGACA NGT 2 0.0072 Tier III
14 NC_000073.6 7 68350110 + Gm16158 NNNGGGCCACACATTTGACC NGG 2 0.0 Tier III
15 NC_000075.6 9 44072077 + Gm39325 NNNGGGCCCCACAGTGGACA NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)