Construct: sgRNA BRDN0001145617
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCCCATAACTACCTAAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK4 (2868)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77725
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
3007756 |
+ |
GRK4 |
NNNCCATAACTACCTAAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
152014762 |
+ |
GALNTL5 |
NNNACATAACTACCTAAGAC |
NGG |
2 |
0.3609 |
Tier I |
| 3 |
NC_000007.14 |
7 |
117540791 |
- |
CFTR |
NNNCCAGAACTACCTAAGAG |
NGG |
1 |
0.5882 |
Tier II |
| 4 |
NC_000006.12 |
6 |
47564995 |
- |
CD2AP |
NNNCCATTACTACATAAGAG |
NGG |
2 |
0.15 |
Tier II |
| 5 |
NC_000009.12 |
9 |
117159803 |
- |
ASTN2 |
NNNCCATAACTAGCTAAGAG |
NAG |
2 |
0.0354 |
Tier II |
| 6 |
NC_000002.12 |
2 |
195325644 |
+ |
LOC105376755 |
NNNCCATAACTACCAAAAAG |
NGG |
2 |
0.4008 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
123720102 |
+ |
Zcchc8 |
NNNCCAAAACTACCTAAGAA |
NGG |
2 |
0.8203 |
Tier II |
| 2 |
NC_000067.6 |
1 |
160204832 |
+ |
Cacybp |
NNNCCAGAACTACCTAAGAA |
NGG |
2 |
0.5515 |
Tier II |
| 3 |
NC_000077.6 |
11 |
79125769 |
+ |
Ksr1 |
NNNCCCTAACAACCTAAGAG |
NGG |
2 |
0.3409 |
Tier II |
| 4 |
NC_000070.6 |
4 |
80572387 |
- |
Gm32658 |
NNNCCATAACTACCAAAGGG |
NGG |
2 |
0.2171 |
Tier III |
| 5 |
NC_000069.6 |
3 |
102271173 |
+ |
A230001M10Rik |
NNNCCATAAATACCTAGGAG |
NGG |
2 |
0.1529 |
Tier III |
Other clones with same target sequence:
(none)
