Construct: sgRNA BRDN0001145631
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATATGTCCACTTACACACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGGY (55277)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77109
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
59456978 |
- |
FGGY |
NNNTGTCCACTTACACACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
161974576 |
+ |
PRKN |
NNNTGTTCACTCACACACCA |
NGG |
2 |
0.7697 |
Tier II |
| 3 |
NC_000021.9 |
21 |
21297619 |
+ |
NCAM2 |
NNNTGCCCACTTACACATCA |
NGG |
2 |
0.5844 |
Tier II |
| 4 |
NC_000014.9 |
14 |
68501540 |
+ |
RAD51B |
NNNTGTCCTTTTACACACCA |
NGG |
2 |
0.5647 |
Tier II |
| 5 |
NC_000002.12 |
2 |
106539085 |
+ |
CD8B2 |
NNNTGTTCACTTACACACCT |
NGG |
2 |
0.4875 |
Tier II |
| 6 |
NC_000004.12 |
4 |
109572236 |
+ |
MCUB |
NNNTGTACACTTACACACTA |
NGG |
2 |
0.3462 |
Tier II |
| 7 |
NC_000002.12 |
2 |
159895911 |
+ |
LY75 |
NNNTGGCCTCTTACACACCA |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000002.12 |
2 |
159895911 |
+ |
LY75-CD302 |
NNNTGGCCTCTTACACACCA |
NGG |
2 |
0.3429 |
Tier II |
| 9 |
NC_000007.14 |
7 |
151965171 |
+ |
GALNTL5 |
NNNTGTCCACTGACTCACCA |
NGG |
2 |
0.1 |
Tier II |
| 10 |
NC_000023.11 |
X |
12297119 |
+ |
FRMPD4 |
NNNTGTCCATTTACACACCA |
NGA |
2 |
0.0654 |
Tier II |
| 11 |
NC_000012.12 |
12 |
116810359 |
- |
RNFT2 |
NNNTTTCCACTTCCACACCA |
NGG |
2 |
0.0632 |
Tier II |
| 12 |
NC_000017.11 |
17 |
68520209 |
+ |
PRKAR1A |
NNNTGTCCACTTACATTCCA |
NGG |
2 |
0.041 |
Tier II |
| 13 |
NC_000017.11 |
17 |
33327610 |
- |
ASIC2 |
NNNTGTCCATTTACACACCA |
NGT |
2 |
0.0152 |
Tier II |
| 14 |
NC_000011.10 |
11 |
28288988 |
+ |
METTL15 |
NNNTGTCCACTTACACAGCA |
NTG |
2 |
0.0052 |
Tier II |
| 15 |
NC_000003.12 |
3 |
38003725 |
- |
VILL |
NNNTGTCCACTTACACACCA |
NAC |
2 |
0.0 |
Tier II |
| 16 |
NC_000005.10 |
5 |
56109287 |
- |
ANKRD55 |
NNNTGTCCACTTAGACACCA |
NTG |
2 |
0.0 |
Tier II |
| 17 |
NC_000003.12 |
3 |
15957042 |
- |
LOC107986064 |
NNNTGCACACTTACACACCA |
NGG |
2 |
0.6818 |
Tier III |
| 18 |
NC_000002.12 |
2 |
40772641 |
+ |
LOC105374497 |
NNNTATCCACCTACACACCA |
NGG |
2 |
0.5778 |
Tier III |
| 19 |
NC_000006.12 |
6 |
70378071 |
- |
LOC105377848 |
NNNTGTCCACATATACACCA |
NGG |
2 |
0.55 |
Tier III |
| 20 |
NC_000004.12 |
4 |
18417862 |
- |
LOC105374510 |
NNNGGTCCACTTCCACACCA |
NGG |
2 |
0.1316 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
95696977 |
- |
Fggy |
NNNTGTCCATTTGCACACCA |
NGG |
2 |
0.6138 |
Tier I |
| 2 |
NC_000067.6 |
1 |
59323438 |
+ |
Cdk15 |
NNNTCTCCACTTACACACCA |
NGG |
1 |
0.7857 |
Tier II |
| 3 |
NC_000069.6 |
3 |
141476376 |
- |
Unc5c |
NNNTCCCCACTTACACACCA |
NGG |
2 |
0.7143 |
Tier II |
| 4 |
NC_000078.6 |
12 |
32059821 |
+ |
Prkar2b |
NNNTGTCCATTTGCACACCA |
NGG |
2 |
0.6138 |
Tier II |
| 5 |
NC_000068.7 |
2 |
5424890 |
- |
Camk1d |
NNNTGTCCACAAACACACCA |
NGG |
2 |
0.6 |
Tier II |
| 6 |
NC_000085.6 |
19 |
26663503 |
+ |
Smarca2 |
NNNTCTCCACATACACACCA |
NGG |
2 |
0.5893 |
Tier II |
| 7 |
NC_000083.6 |
17 |
85987062 |
+ |
Srbd1 |
NNNTGTACACTTACACATCA |
NGG |
2 |
0.4821 |
Tier II |
| 8 |
NC_000067.6 |
1 |
87670347 |
- |
Inpp5d |
NNNTGTCCCCATACACACCA |
NGG |
2 |
0.4286 |
Tier II |
| 9 |
NC_000073.6 |
7 |
42264251 |
+ |
Vmn2r61 |
NNNTGTCCCCTGACACACCA |
NGG |
2 |
0.2857 |
Tier II |
| 10 |
NC_000077.6 |
11 |
47954498 |
+ |
Sgcd |
NNNTGTGCACTTACACACCT |
NGG |
2 |
0.2824 |
Tier II |
| 11 |
NC_000068.7 |
2 |
106202172 |
+ |
Dcdc5 |
NNNTGTGCTCTTACACACCA |
NGG |
2 |
0.2824 |
Tier II |
| 12 |
NC_000073.6 |
7 |
31713101 |
+ |
Scgb1b7 |
NNNTGTGCTCTTACACACCA |
NGG |
2 |
0.2824 |
Tier II |
| 13 |
NC_000073.6 |
7 |
32441960 |
+ |
Scgb1b29 |
NNNTGTGCTCTTACACACCA |
NGG |
2 |
0.2824 |
Tier II |
| 14 |
NC_000076.6 |
10 |
116458249 |
+ |
Kcnmb4 |
NNNTGTCCACTCACCCACCA |
NGG |
2 |
0.2584 |
Tier II |
| 15 |
NC_000070.6 |
4 |
85827589 |
- |
Adamtsl1 |
NNNTGTCCAGTTACACATCA |
NGG |
2 |
0.25 |
Tier II |
| 16 |
NC_000084.6 |
18 |
65626582 |
- |
Zfp532 |
NNNTGTCCACTGACACACAA |
NGG |
2 |
0.2143 |
Tier II |
| 17 |
NC_000084.6 |
18 |
65677777 |
- |
Zfp532 |
NNNTGTCCACTCCCACACCA |
NGG |
2 |
0.1994 |
Tier II |
| 18 |
NC_000080.6 |
14 |
60523461 |
+ |
Spata13 |
NNNTGTCCACTCACTCACCA |
NGG |
2 |
0.1895 |
Tier II |
| 19 |
NC_000082.6 |
16 |
72440184 |
+ |
Robo1 |
NNNTGTCCACTTGCACACCA |
NAG |
2 |
0.1691 |
Tier II |
| 20 |
NC_000072.6 |
6 |
89351289 |
- |
Plxna1 |
NNNTGTCCACTTACACTCCA |
NGG |
1 |
0.1333 |
Tier II |
| 21 |
NC_000067.6 |
1 |
181361979 |
+ |
Cnih3 |
NNNTGTCCCCTTACACCCCA |
NGG |
2 |
0.1008 |
Tier II |
| 22 |
NC_000069.6 |
3 |
90333969 |
+ |
Gatad2b |
NNNTGTCCACATACACACCA |
NGA |
2 |
0.0521 |
Tier II |
| 23 |
NC_000080.6 |
14 |
77311844 |
+ |
Enox1 |
NNNTTTCCACTTACACACCA |
NTG |
2 |
0.0117 |
Tier II |
| 24 |
NC_000079.6 |
13 |
110010474 |
- |
Gm32881 |
NNNTGTCCACTGACACAACA |
NGG |
2 |
0.2692 |
Tier III |
| 25 |
NC_000068.7 |
2 |
28127088 |
- |
F730016J06Rik |
NNNTTTCCACTTTCACACCA |
NGG |
2 |
0.09 |
Tier III |
Other clones with same target sequence:
(none)
