Construct: sgRNA BRDN0001145637
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCACTAGGTGAACGTATTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRIM24 (8805)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77887
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
138576405 |
- |
TRIM24 |
NNNCTAGGTGAACGTATTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
63606881 |
+ |
WDR89 |
NNNCTAAGTGAACATATTGG |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000015.10 |
15 |
78033648 |
+ |
TBC1D2B |
NNNATAGGTGAACTTATTGG |
NGG |
2 |
0.2246 |
Tier II |
| 4 |
NC_000022.11 |
22 |
25021023 |
+ |
KIAA1671 |
NNNCTAGGTGAATGGATTGG |
NGG |
2 |
0.035 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
37956435 |
- |
Trim24 |
NNNCTAGGTGAACGTATTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
106541350 |
+ |
Tex2 |
NNNCTGGGTGAACATATTGG |
NGG |
2 |
0.5357 |
Tier II |
| 3 |
NC_000083.6 |
17 |
6192339 |
+ |
Tulp4 |
NNNCTAGGTGAATGTATTGT |
NGG |
2 |
0.49 |
Tier II |
| 4 |
NC_000069.6 |
3 |
94345404 |
- |
Them5 |
NNNCTAGGAGAAAGTATTGG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000075.6 |
9 |
86839288 |
- |
Snap91 |
NNNCTAGGTGAATGTATTCG |
NGG |
2 |
0.3138 |
Tier II |
| 6 |
NC_000079.6 |
13 |
63517651 |
+ |
Ptch1 |
NNNCTAGGTGACCATATTGG |
NGG |
2 |
0.1974 |
Tier II |
| 7 |
NC_000073.6 |
7 |
122348768 |
+ |
Prkcb |
NNNCTAGGTGAACTTAGTGG |
NGG |
2 |
0.0314 |
Tier II |
Other clones with same target sequence:
(none)
