Construct: sgRNA BRDN0001145638
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGTTTGACCACATTCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKK1 (84254)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76514
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
3883064 |
+ |
CAMKK1 |
NNNGTTTGACCACATTCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
233848449 |
+ |
HJURP |
NNNGCTTGACCACATTCACA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000011.10 |
11 |
92463949 |
- |
FAT3 |
NNNATTTTACCACATTCACG |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000005.10 |
5 |
73772585 |
+ |
ARHGEF28 |
NNNGTTTTACCACATTCACC |
NGG |
2 |
0.2679 |
Tier II |
| 5 |
NC_000007.14 |
7 |
129364497 |
+ |
AHCYL2 |
NNNGTTTCACCACATTCACC |
NGG |
2 |
0.2637 |
Tier II |
| 6 |
NC_000006.12 |
6 |
39801803 |
+ |
DAAM2 |
NNNGTCTGACCACATTCCCG |
NGG |
2 |
0.1732 |
Tier II |
| 7 |
NC_000017.11 |
17 |
76064451 |
- |
SRP68 |
NNNGTTTGACAACATTCAGG |
NGG |
2 |
0.0938 |
Tier II |
| 8 |
NC_000007.14 |
7 |
87431166 |
+ |
ABCB4 |
NNNGTTTGACCACATTCCCC |
NGG |
2 |
0.0816 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
73027804 |
- |
Camkk1 |
NNNATTTGACTACATTCACG |
NGG |
2 |
0.2769 |
Tier I |
| 2 |
NC_000073.6 |
7 |
119993676 |
+ |
Dnah3 |
NNNGTTTGAGCACATTCACA |
NGG |
2 |
0.3646 |
Tier II |
| 3 |
NC_000082.6 |
16 |
56080369 |
- |
Senp7 |
NNNGTTGGACCACATTCACC |
NGG |
2 |
0.2521 |
Tier II |
| 4 |
NC_000072.6 |
6 |
142850077 |
- |
St8sia1 |
NNNGTTTGACCACAGTCATG |
NGG |
2 |
0.0231 |
Tier II |
| 5 |
NC_000078.6 |
12 |
80944042 |
- |
Gm20337 |
NNNGTCTGACCTCATTCACG |
NGG |
2 |
0.303 |
Tier III |
Other clones with same target sequence:
(none)
