Construct: sgRNA BRDN0001145643
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGAGCACTTGGTAATCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3CB (5291)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76193
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
138691035 |
- |
PIK3CB |
NNNGAGCACTTGGTAATCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
47166850 |
- |
MDGA2 |
NNNGAGCATTTGGTAATCAG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000002.12 |
2 |
10126822 |
+ |
RRM2 |
NNNTAGCAGTTGGTAATCGG |
NGG |
2 |
0.2251 |
Tier II |
| 4 |
NC_000022.11 |
22 |
45562477 |
+ |
FBLN1 |
NNNGAGCACTTGGTAAACGG |
NAG |
2 |
0.1383 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
99061766 |
- |
Pik3cb |
NNNGAGCACTTGATAATCGG |
NGG |
1 |
0.9231 |
Tier I |
| 2 |
NC_000070.6 |
4 |
128309223 |
- |
Csmd2 |
NNNGAGCACTTGGTGATCTG |
NGG |
2 |
0.4333 |
Tier II |
| 3 |
NC_000074.6 |
8 |
94047671 |
- |
Ogfod1 |
NNNCAGCACTTGGTAATCTG |
NGG |
2 |
0.3529 |
Tier II |
| 4 |
NC_000069.6 |
3 |
154020865 |
+ |
Slc44a5 |
NNNGAGCACTTGTTAATCAG |
NGG |
2 |
0.2143 |
Tier II |
| 5 |
NC_000067.6 |
1 |
12706447 |
- |
Sulf1 |
NNNGAGCACTTGGTAAGCGG |
NCG |
2 |
0.0126 |
Tier II |
| 6 |
NC_000075.6 |
9 |
62873983 |
+ |
Calml4 |
NNNGAGCACTTGGGAATCAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000067.6 |
1 |
92753642 |
- |
Gm36102 |
NNNGAGCTCTTGGTAATCGG |
NCG |
2 |
0.0459 |
Tier III |
Other clones with same target sequence:
(none)
