Construct: sgRNA BRDN0001145644
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGATGTATATCAGCATAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK3 (6788)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75976
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
98706491 |
+ |
STK3 |
NNNTGTATATCAGCATAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
44995234 |
- |
STK4 |
NNNTGGATATCAGCATAAGG |
NGG |
1 |
0.5714 |
Tier I |
| 3 |
NC_000001.11 |
1 |
169281340 |
+ |
NME7 |
NNNTGCTTATCAGCATAAGG |
NGG |
2 |
0.3977 |
Tier II |
| 4 |
NC_000004.12 |
4 |
154330041 |
- |
DCHS2 |
NNNTGTATAGCAGCATAAAG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000014.9 |
14 |
88755555 |
+ |
EML5 |
NNNTGTACATCAGCAGAAGG |
NGG |
2 |
0.3462 |
Tier II |
| 6 |
NC_000004.12 |
4 |
26127604 |
+ |
RBPJ |
NNNAGTATATCAGCATAAAG |
NGG |
2 |
0.3401 |
Tier II |
| 7 |
NC_000021.9 |
21 |
36575674 |
+ |
CLDN14 |
NNNTGTTTATCAGCATAAGT |
NGG |
2 |
0.3062 |
Tier II |
| 8 |
NC_000002.12 |
2 |
133169063 |
+ |
NCKAP5 |
NNNAGTATTTCAGCATAAGG |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000013.11 |
13 |
69936303 |
+ |
KLHL1 |
NNNTGTATATCAGAATATGG |
NGG |
2 |
0.175 |
Tier II |
| 10 |
NC_000003.12 |
3 |
174125398 |
+ |
NLGN1 |
NNNTGTATATGAGCACAAGG |
NGG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000011.10 |
11 |
21201514 |
+ |
NELL1 |
NNNTGTTTATCAGCTTAAGG |
NGG |
2 |
0.0875 |
Tier II |
| 12 |
NC_000013.11 |
13 |
77203171 |
- |
MYCBP2 |
NNNTGTTTATCAGCTTAAGG |
NGG |
2 |
0.0875 |
Tier II |
| 13 |
NC_000017.11 |
17 |
61825500 |
- |
BRIP1 |
NNNTGTTTATCAGCTTAAGG |
NGG |
2 |
0.0875 |
Tier II |
| 14 |
NC_000004.12 |
4 |
17663492 |
- |
FAM184B |
NNNTGTTTATCAGCATAAGG |
NGA |
2 |
0.0304 |
Tier II |
| 15 |
NC_000003.12 |
3 |
66294405 |
+ |
SLC25A26 |
NNNTGTTTATCAGCATAAGG |
NGC |
2 |
0.0097 |
Tier II |
| 16 |
NC_000023.11 |
X |
36110992 |
+ |
CFAP47 |
NNNTGTTTATCAGCATAAGG |
NGT |
2 |
0.0071 |
Tier II |
| 17 |
NC_000002.12 |
2 |
231120345 |
+ |
HTR2B |
NNNTTTATATCAGCATAAGG |
NGT |
2 |
0.0048 |
Tier II |
| 18 |
NC_000002.12 |
2 |
231120345 |
+ |
PSMD1 |
NNNTTTATATCAGCATAAGG |
NGT |
2 |
0.0048 |
Tier II |
| 19 |
NC_000001.11 |
1 |
86101273 |
+ |
COL24A1 |
NNNTGTATATCAGCTTAAGG |
NGC |
2 |
0.0044 |
Tier II |
| 20 |
NC_000016.10 |
16 |
74880071 |
- |
WDR59 |
NNNTGTATATCATGATAAGG |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000003.12 |
3 |
182001300 |
+ |
LINC01206 |
NNNTGAATATCAGCATAAGG |
NGG |
1 |
0.8667 |
Tier III |
| 22 |
NC_000018.10 |
18 |
42383561 |
+ |
LINC00907 |
NNNTGTATATCAGCATAATG |
NGG |
1 |
0.6667 |
Tier III |
| 23 |
NC_000013.11 |
13 |
78559612 |
+ |
OBI1-AS1 |
NNNTGTATATCAACATAGGG |
NGG |
2 |
0.3692 |
Tier III |
| 24 |
NC_000002.12 |
2 |
56597128 |
- |
LOC101927213 |
NNNTGTATACCACCATAAGG |
NGG |
2 |
0.2246 |
Tier III |
| 25 |
NC_000006.12 |
6 |
2290811 |
- |
GMDS-DT |
NNNTGTTTATCAGCTTAAGG |
NGG |
2 |
0.0875 |
Tier III |
| 26 |
NC_000010.11 |
10 |
95166091 |
- |
LOC107984257 |
NNNTGTTTATCAGCTTAAGG |
NGG |
2 |
0.0875 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
35072454 |
+ |
Stk3 |
NNNTGTATATCAGCATAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
164096883 |
- |
Stk4 |
NNNTGGATATCAGCATATGG |
NGG |
2 |
0.2857 |
Tier I |
| 3 |
NC_000069.6 |
3 |
122154440 |
+ |
Abca4 |
NNNTGTACATCAGCATAAGA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000075.6 |
9 |
35102104 |
+ |
St3gal4 |
NNNTGTATATCAGCAAAAAG |
NGG |
2 |
0.6494 |
Tier II |
| 5 |
NC_000080.6 |
14 |
75033720 |
+ |
Rubcnl |
NNNTGTAGAGCAGCATAAGG |
NGG |
2 |
0.3667 |
Tier II |
| 6 |
NC_000074.6 |
8 |
108676958 |
+ |
Zfhx3 |
NNNTGTATATCTCCATAAGG |
NGG |
2 |
0.1404 |
Tier II |
| 7 |
NC_000072.6 |
6 |
80306108 |
+ |
Lrrtm4 |
NNNTGTATATCTGCTTAAGG |
NGG |
2 |
0.0667 |
Tier II |
| 8 |
NC_000074.6 |
8 |
70739223 |
- |
Pde4c |
NNNTGTATATGAGCATAAGG |
NAG |
2 |
0.0648 |
Tier II |
| 9 |
NC_000077.6 |
11 |
119404556 |
+ |
Rnf213 |
NNNTGTATATCAGCATAAAG |
NGT |
2 |
0.0115 |
Tier II |
| 10 |
NC_000074.6 |
8 |
127400014 |
+ |
Pard3 |
NNNTGTATATCAGCATAAGT |
NGT |
2 |
0.0113 |
Tier II |
| 11 |
NC_000084.6 |
18 |
56557956 |
- |
Aldh7a1 |
NNNTGTATATCAGCATAAGG |
NTA |
2 |
0.0 |
Tier II |
| 12 |
NC_000072.6 |
6 |
31222698 |
+ |
2210408F21Rik |
NNNTGTATATCTGCATAAGG |
NTG |
2 |
0.013 |
Tier III |
Other clones with same target sequence:
(none)
