Construct: sgRNA BRDN0001145648

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGGAACTTTGGGCCACCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FXN (2395)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77814

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 69046478 + FXN NNNGAACTTTGGGCCACCCA NGG 0 1.0 Tier I
2 NC_000003.12 3 11256171 + HRH1 NNNGGACTTTGGGTCACCCA NGG 2 0.528 Tier II
3 NC_000001.11 1 2158726 - PRKCZ NNNGAGCTTTGGGCCACTCA NGG 2 0.4592 Tier II
4 NC_000008.11 8 123020645 - DERL1 NNNGAACTTTTGGCCAACCA NGG 2 0.1795 Tier II
5 NC_000002.12 2 46599063 + PIGF NNNGTACTTTGGGCCAACCA NGG 2 0.1697 Tier II
6 NC_000010.11 10 48428597 + MAPK8 NNNGAACTTTGGGAAACCCA NGG 2 0.0778 Tier II
7 NC_000003.12 3 140354264 + CLSTN2 NNNGAACTTTGGGCTACTCA NGG 2 0.0429 Tier II
8 NC_000009.12 9 124968669 - SCAI NNNGAACTTTGGGCCACCCA NGC 1 0.0222 Tier II
9 NC_000022.11 22 44735918 + PRR5 NNNGAACTTTGAGCCACCCA NGC 2 0.0207 Tier II
10 NC_000022.11 22 44735918 + PRR5-ARHGAP8 NNNGAACTTTGAGCCACCCA NGC 2 0.0207 Tier II
11 NC_000002.12 2 200337392 + SPATS2L NNNGAACTTTGGGCCAGCCA NAG 2 0.0153 Tier II
12 NC_000004.12 4 186211606 - KLKB1 NNNGAACTTTGGGCCAGGCA NGG 2 0.0078 Tier II
13 NC_000004.12 4 186211606 - CYP4V2 NNNGAACTTTGGGCCAGGCA NGG 2 0.0078 Tier II
14 NC_000014.9 14 33744512 + NPAS3 NNNGAACTTTGGGCCAGGCA NGG 2 0.0078 Tier II
15 NC_000002.12 2 208012474 + PLEKHM3 NNNGTACTTTGGGCCACCCA NGT 2 0.0059 Tier II
16 NC_000022.11 22 30608161 + TCN2 NNNGAACTTTGGGCCGCCCA NGC 2 0.0043 Tier II
17 NC_000002.12 2 183124625 - NUP35 NNNGAACTTGGGGGCACCCA NGG 2 0.0 Tier II
18 NC_000020.11 20 48634496 - PREX1 NNNGAACTTTGGGGCACACA NGG 2 0.0 Tier II
19 NC_000016.10 16 71594403 + LOC105371334 NNNGAACTAGGGGCCACCCA NGG 2 0.4643 Tier III
20 NC_000011.10 11 112732887 + LOC105369496 NNNGAACTTTGGCCCACCCA NGG 1 0.4211 Tier III
21 NC_000008.11 8 123020645 - LOC124902013 NNNGAACTTTTGGCCAACCA NGG 2 0.1795 Tier III
22 NC_000002.12 2 46599063 + LOC124906003 NNNGTACTTTGGGCCAACCA NGG 2 0.1697 Tier III
23 NC_000012.12 12 48015126 + LOC105369750 NNNGAACTATGGGCCAGCCA NGG 2 0.0546 Tier III
24 NC_000009.12 9 124968669 - FXNP2 NNNGAACTTTGGGCCACCCA NGC 1 0.0222 Tier III
25 NC_000002.12 2 183124625 - LOC124907917 NNNGAACTTGGGGGCACCCA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 107547931 - 1700028K03Rik NNNGAATTTTAGGCCACCCA NGG 2 0.8125 Tier II
2 NC_000079.6 13 11949133 - Ryr2 NNNGAACTTAGTGCCACCCA NGG 2 0.3297 Tier II
3 NC_000080.6 14 31180114 + Nisch NNNGACCTTTGTGCCACCCA NGG 2 0.1748 Tier II
4 NC_000083.6 17 32156448 - Notch3 NNNGAACTTTGGGCCACCCC NAG 2 0.0589 Tier II
5 NC_000071.6 5 115497741 - Pxn NNNGAACTCTGGGCCAGCCA NGG 2 0.0543 Tier II
6 NC_000073.6 7 125711770 - D430042O09Rik NNNGAACTTAGGGCCAGCCA NGG 2 0.0504 Tier II
7 NC_000083.6 17 35225415 - Nfkbil1 NNNGAAATTTGGGCTACCCA NGG 2 0.05 Tier II
8 NC_000078.6 12 86951087 - Cipc NNNGAGCTTTGGGCCAGCCA NGG 2 0.042 Tier II
9 NC_000081.6 15 20842792 + Cdh12 NNNGAACTTTGAGCCACCCA NTG 2 0.0364 Tier II
10 NC_000078.6 12 83224409 - Dpf3 NNNGAACTTTGGGCCAGCCT NGG 2 0.0353 Tier II
11 NC_000081.6 15 78289662 - Csf2rb2 NNNGAGCTTTGGGCCACCCA NGC 2 0.0159 Tier II
12 NC_000073.6 7 140198842 + Cd163l1 NNNGAACTTTGGGCCAGCCA NGT 2 0.0009 Tier II
13 NC_000073.6 7 36176861 + Gm46016 NNNGAACTGTGGGCCACTCA NGG 2 0.398 Tier III
14 NC_000084.6 18 13974230 - 6330525I24Rik NNNGAACTTTGGACCACCCA NGC 2 0.0205 Tier III
Download CSV

Other clones with same target sequence:

(none)