Construct: sgRNA BRDN0001145650

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTGAGGAAGACTCAATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RP2 (6102)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76283

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 46853782 - RP2 NNNGAGGAAGACTCAATGAT NGG 0 1.0 Tier I
2 NC_000006.12 6 21010512 + CDKAL1 NNNGAGAAAGAGTCAATGAT NGG 2 0.4444 Tier II
3 NC_000002.12 2 100284166 + LONRF2 NNNGAGGAGGACTCAATGTT NGG 2 0.359 Tier II
4 NC_000011.10 11 16961378 + PLEKHA7 NNNGAGTAAGACTCAAAGAT NGG 2 0.3048 Tier II
5 NC_000003.12 3 85978679 - CADM2 NNNTAGGAAGACTTAATGAT NGG 2 0.2667 Tier II
6 NC_000001.11 1 121342501 + SRGAP2C NNNGAAGAAGACTCAATGAT NAG 2 0.2593 Tier II
7 NC_000001.11 1 144937574 - SRGAP2B NNNGAAGAAGACTCAATGAT NAG 2 0.2593 Tier II
8 NC_000001.11 1 206361150 + SRGAP2 NNNGAAGAAGACTCAATGAT NAG 2 0.2593 Tier II
9 NC_000006.12 6 71308216 + OGFRL1 NNNGAGAAAGACTCAGTGAT NGG 2 0.1923 Tier II
10 NC_000016.10 16 55681275 + SLC6A2 NNNGAGAAAGACTCAGTGAT NGG 2 0.1923 Tier II
11 NC_000018.10 18 23622588 - ANKRD29 NNNGAGCAAGACTCCATGAT NGG 2 0.1875 Tier II
12 NC_000003.12 3 50688839 - DOCK3 NNNGGGGAAGACTCAATGAT NAG 2 0.1867 Tier II
13 NC_000010.11 10 125911766 - FANK1 NNNGAGGAAGACTCAATAAT NAG 2 0.1795 Tier II
14 NC_000002.12 2 207556851 + CREB1 NNNGAGGAAGACACAATGCT NGG 2 0.1432 Tier II
15 NC_000010.11 10 59268417 + FAM13C NNNGATGAAGACTCTATGAT NGG 2 0.1333 Tier II
16 NC_000016.10 16 23634008 - PALB2 NNNGAGGAAGACTCTATGTT NGG 2 0.1077 Tier II
17 NC_000011.10 11 12206876 - MICAL2 NNNCAGGAAGACTCAGTGAT NGG 2 0.1018 Tier II
18 NC_000012.12 12 24177835 - SOX5 NNNGAGAAAGACTCAATGAC NGG 2 0.0909 Tier II
19 NC_000013.11 13 41696830 - VWA8 NNNGAGGAAGAATCAATGAT NTG 2 0.0278 Tier II
20 NC_000016.10 16 17359633 - XYLT1 NNNGAGGAAGACTCAATGCC NGG 2 0.0188 Tier II
21 NC_000007.14 7 40490146 + SUGCT NNNGAGGAAGACTCAATGAG NGC 2 0.0039 Tier II
22 NC_000005.10 5 50739229 - PARP8 NNNGAGGAAGACTGAATGAC NGG 2 0.0 Tier II
23 NC_000005.10 5 89584322 + LINC02161 NNNGAGGAAGAAACAATGAT NGG 2 0.4945 Tier III
24 NC_000018.10 18 39320394 + MIR924HG NNNGAGGAAGACACAATGGT NGG 2 0.2596 Tier III
25 NC_000018.10 18 39320394 + LOC101927879 NNNGAGGAAGACACAATGGT NGG 2 0.2596 Tier III
26 NC_000006.12 6 71308216 + LOC124901339 NNNGAGAAAGACTCAGTGAT NGG 2 0.1923 Tier III
27 NC_000007.14 7 6667364 - LOC124901583 NNNGAGGAAGACACAATGAG NGG 2 0.1222 Tier III
28 NC_000023.11 X 17979478 + LINC01456 NNNTAGGAAGACTCAATGAT NAG 2 0.0943 Tier III
29 NC_000008.11 8 33931903 + LOC105379364 NNNGAGTAAGACTCAATGAT NGT 2 0.0092 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 105180038 + Tcl1b5 NNNGAGGATGACTGAATGAT NGG 2 0.0 Tier I
2 NC_000067.6 1 156192098 - Fam163a NNNGAGAAAGAATCAATGAT NGG 2 0.7143 Tier II
3 NC_000072.6 6 24098295 + Slc13a1 NNNAAGGGAGACTCAATGAT NGG 2 0.66 Tier II
4 NC_000072.6 6 80056334 - Lrrtm4 NNNGAAGAAGACTCAAAGAT NGG 2 0.5333 Tier II
5 NC_000076.6 10 19888976 + Pex7 NNNGAGGAAAACTCAATGAT NAG 2 0.242 Tier II
6 NC_000083.6 17 50083902 - Rftn1 NNNGAGGATCACTCAATGAT NGG 2 0.24 Tier II
7 NC_000073.6 7 112292544 + Mical2 NNNGTGGAAGACTCAATGAA NGG 2 0.2045 Tier II
8 NC_000078.6 12 28479052 - Dcdc2c NNNGAGAAAGACTCTATGAT NGG 2 0.2 Tier II
9 NC_000086.7 X 98572185 - Ophn1 NNNAAGGAAGACTCTATGAT NGG 2 0.18 Tier II
10 NC_000074.6 8 105780139 - Ranbp10 NNNGAGGTAGACTAAATGAT NGG 2 0.15 Tier II
11 NC_000071.6 5 65453095 - Smim14 NNNGAGGAAGACTCAATTCT NGG 2 0.1379 Tier II
12 NC_000082.6 16 91559334 + Ifngr2 NNNGAGGAAAACTCAATGAT NCG 2 0.1 Tier II
13 NC_000070.6 4 35878191 + Lingo2 NNNGAGGAAGAATCAAGGAT NGG 2 0.084 Tier II
14 NC_000067.6 1 178419509 - Efcab2 NNNGAGTAAGACTCAATGAT NTG 2 0.0223 Tier II
15 NC_000067.6 1 84589065 - Dner NNNGAGGAAGAGTCACTGAT NGG 2 0.0 Tier II
16 NC_000069.6 3 135306254 + Gm46826 NNNGAGTAAGACTCAAAGAT NGG 2 0.3048 Tier III
17 NC_000076.6 10 43624744 + F930017D23Rik NNNGAGGAAGACTCCATGAT NGG 1 0.2727 Tier III
18 NC_000074.6 8 50149881 - 1700019L22Rik NNNGAGGAAGACTGAAGGAT NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)