Construct: sgRNA BRDN0001145651
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATAGCATATCCATTGAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K2 (51447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77819
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48693114 |
- |
IP6K2 |
NNNAGCATATCCATTGAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
11024513 |
+ |
TARDBP |
NNNAGCATATCAATTGAAAA |
NGG |
2 |
0.6696 |
Tier II |
| 3 |
NC_000012.12 |
12 |
40548801 |
+ |
MUC19 |
NNNAGCATAACCAATGAAAG |
NGG |
2 |
0.5306 |
Tier II |
| 4 |
NC_000007.14 |
7 |
80152474 |
+ |
GNAI1 |
NNNTGCATATCCATTGAAAT |
NGG |
2 |
0.4455 |
Tier II |
| 5 |
NC_000013.11 |
13 |
96464489 |
- |
HS6ST3 |
NNNAGGATATACATTGAAAG |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000008.11 |
8 |
13087951 |
+ |
DLC1 |
NNNAGCATATTCATTGGAAG |
NGG |
2 |
0.0543 |
Tier II |
| 7 |
NC_000006.12 |
6 |
47719988 |
+ |
ADGRF4 |
NNNAGCATATCCCTTGCAAG |
NGG |
2 |
0.0372 |
Tier II |
| 8 |
NC_000007.14 |
7 |
130121750 |
- |
KLHDC10 |
NNNAGCATTTCCATTGAAAG |
NTG |
2 |
0.0234 |
Tier II |
| 9 |
NC_000015.10 |
15 |
76612024 |
+ |
SCAPER |
NNNAGCATATCCCTTGAAAG |
NCG |
2 |
0.0226 |
Tier II |
| 10 |
NC_000006.12 |
6 |
4891100 |
+ |
CDYL |
NNNAGCATCTCCATTGAAAG |
NTG |
2 |
0.0223 |
Tier II |
| 11 |
NC_000003.12 |
3 |
133673586 |
+ |
TF |
NNNAGCATCTCCAGTGAAAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000022.11 |
22 |
32302174 |
- |
SLC5A4 |
NNNAGGATATCCATTTAAAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000002.12 |
2 |
14371390 |
+ |
LINC00276 |
NNNAGCATTTCCATTGAAAA |
NGG |
2 |
0.5625 |
Tier III |
| 14 |
NC_000002.12 |
2 |
51673606 |
+ |
NRXN1-DT |
NNNAGCATCTCCATTGAAAT |
NGG |
2 |
0.4 |
Tier III |
| 15 |
NC_000003.12 |
3 |
118738713 |
- |
LOC105374060 |
NNNAGCATATTCATTGAAAG |
NAG |
2 |
0.0798 |
Tier III |
| 16 |
NC_000016.10 |
16 |
55437294 |
+ |
MMP2-AS1 |
NNNAGCAGATCCATTTAAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108798337 |
+ |
Ip6k2 |
NNNAGCATATCCATTAAAAG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000086.7 |
X |
10067491 |
- |
Srpx |
NNNAGGATATACATTGAAAG |
NGG |
2 |
0.375 |
Tier I |
| 3 |
NC_000081.6 |
15 |
55603462 |
+ |
Mtbp |
NNNAGCATGTCCATTAAAAG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000073.6 |
7 |
109743061 |
- |
Tmem9b |
NNNAGCATTTCCATTGAAAG |
NGG |
1 |
0.6 |
Tier II |
| 5 |
NC_000085.6 |
19 |
39630103 |
+ |
Cyp2c67 |
NNNAGCATATCTATTAAAAG |
NGG |
2 |
0.5385 |
Tier II |
| 6 |
NC_000085.6 |
19 |
39715908 |
+ |
Cyp2c68 |
NNNAGCATATCTATTAAAAG |
NGG |
2 |
0.5385 |
Tier II |
| 7 |
NC_000085.6 |
19 |
39790271 |
+ |
Cyp2c40 |
NNNAGCATATCTATTAAAAG |
NGG |
2 |
0.5385 |
Tier II |
| 8 |
NC_000085.6 |
19 |
39863724 |
+ |
Cyp2c69 |
NNNAGCATATCTATTAAAAG |
NGG |
2 |
0.5385 |
Tier II |
| 9 |
NC_000067.6 |
1 |
37043096 |
+ |
Vwa3b |
NNNAACATATCTATTGAAAG |
NGG |
2 |
0.4667 |
Tier II |
| 10 |
NC_000083.6 |
17 |
42382735 |
+ |
Ptchd4 |
NNNAGGATAACCATTGAAAG |
NGG |
2 |
0.4286 |
Tier II |
| 11 |
NC_000077.6 |
11 |
104521220 |
+ |
Cdc27 |
NNNAGCATATCCATAGAAAT |
NGG |
2 |
0.4053 |
Tier II |
| 12 |
NC_000075.6 |
9 |
104660341 |
- |
Cpne4 |
NNNAGCAAATCCATTGAAAG |
NAG |
2 |
0.2074 |
Tier II |
| 13 |
NC_000067.6 |
1 |
173976267 |
+ |
Ifi202b |
NNNAGCATATTCAATGAAAG |
NGG |
2 |
0.1905 |
Tier II |
| 14 |
NC_000082.6 |
16 |
35352628 |
- |
Sec22a |
NNNAGCATATCCATTGACAG |
NGC |
2 |
0.0042 |
Tier II |
| 15 |
NC_000073.6 |
7 |
16468002 |
- |
Npas1 |
NNNAGCAAATCCATTTAAAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000071.6 |
5 |
19542166 |
- |
Magi2 |
NNNAGCAGATCCATTCAAAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000073.6 |
7 |
82527573 |
+ |
Adamtsl3 |
NNNAGCATAACCATTTAAAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000075.6 |
9 |
58849148 |
- |
Hcn4 |
NNNAGCATATACAGTGAAAG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000067.6 |
1 |
37043096 |
+ |
Gm39626 |
NNNAACATATCTATTGAAAG |
NGG |
2 |
0.4667 |
Tier III |
| 20 |
NC_000075.6 |
9 |
120220476 |
- |
Gm38642 |
NNNAGCATATCCACTGCAAG |
NGG |
2 |
0.0504 |
Tier III |
| 21 |
NC_000070.6 |
4 |
37290912 |
- |
Gm12374 |
NNNAGCATATCCACTGAAAG |
NTG |
2 |
0.0111 |
Tier III |
Other clones with same target sequence:
(none)
