Construct: sgRNA BRDN0001145652

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATATGACACTGCAATTTGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NMRK1 (54981)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75830

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 75077527 + NMRK1 NNNTGACACTGCAATTTGGG NGG 0 1.0 Tier I
2 NC_000012.12 12 56120832 - ZC3H10 NNNTGAAACGGCAATTTGGG NGG 2 0.375 Tier I
3 NC_000010.11 10 48777643 + WDFY4 NNNTAATACTGCAATTTGGG NGG 2 0.7042 Tier II
4 NC_000013.11 13 45712092 + CBY2 NNNGGACACAGCAATTTGGG NGG 2 0.5357 Tier II
5 NC_000004.12 4 87217749 - KLHL8 NNNTGTAACTGCAATTTGGG NGG 2 0.5357 Tier II
6 NC_000021.9 21 44682763 - TSPEAR NNNTGACACTGCATTTTGGA NGG 2 0.5 Tier II
7 NC_000020.11 20 16363840 + KIF16B NNNTGCCACTGAAATTTGGG NGG 2 0.3247 Tier II
8 NC_000014.9 14 99545315 + CCDC85C NNNTGACACTGCAATGTGGA NGG 2 0.3245 Tier II
9 NC_000001.11 1 11204166 - MTOR NNNTGAATCTGCAATTTGGG NGG 2 0.3214 Tier II
10 NC_000020.11 20 51800408 - SALL4 NNNTGCCACTGCGATTTGGG NGG 2 0.2964 Tier II
11 NC_000014.9 14 103050810 + CDC42BPB NNNTGACTCTGCAATTTTGG NGG 2 0.2857 Tier II
12 NC_000003.12 3 56430546 + ERC2 NNNTAACACTGCACTTTGGG NGG 2 0.1857 Tier II
13 NC_000002.12 2 158342290 - CCDC148 NNNTGACACAGCACTTTGGG NGG 2 0.1837 Tier II
14 NC_000003.12 3 33545668 + CLASP2 NNNTCACACTGCACTTTGGG NGG 2 0.1684 Tier II
15 NC_000004.12 4 41161786 - APBB2 NNNTTACACTGGAATTTGGG NGG 2 0.1333 Tier II
16 NC_000004.12 4 182125900 + TENM3 NNNTGACAATGCAATTTGGG NGA 2 0.0595 Tier II
17 NC_000012.12 12 5493684 + NTF3 NNNTGACACTGCAATATGGG NGC 2 0.0202 Tier II
18 NC_000007.14 7 114570465 - FOXP2 NNNTGACACTGCAATTTTGG NGT 2 0.0108 Tier II
19 NC_000018.10 18 39897105 + LINC01901 NNNTCACACTGCAATTTGGG NGG 1 0.7857 Tier III
20 NC_000021.9 21 24944585 - LINC01692 NNNTAATACTGCAATTTGGG NGG 2 0.7042 Tier III
21 NC_000020.11 20 45431658 - LOC105372631 NNNTCAGACTGCAATTTGGG NGG 2 0.3697 Tier III
22 NC_000021.9 21 16180087 + MIR99AHG NNNTGACACAGCAATTTGGG NAG 2 0.2222 Tier III
23 NC_000017.11 17 14844794 + LINC02096 NNNCGACACTGCAATTTGGG NAG 2 0.1678 Tier III
24 NC_000005.10 5 160469436 - MIR3142HG NNNGGACACTGCACTTTGGG NGG 2 0.1339 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 160454131 + Adgrg2 NNNTGACACTAGAATTTGGG NGG 2 0.4444 Tier II
2 NC_000070.6 4 145433227 + Gm42346 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
3 NC_000070.6 4 145588447 + Gm13212 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
4 NC_000070.6 4 145673737 + Zfp980 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
5 NC_000070.6 4 146513793 + Zfp981 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
6 NC_000070.6 4 146513793 + Zfp993 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
7 NC_000070.6 4 146614689 + Zfp993 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
8 NC_000070.6 4 146974953 + Rex2 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
9 NC_000070.6 4 146974953 + Zfp993 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
10 NC_000070.6 4 147135751 + Zfp991 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
11 NC_000070.6 4 147135751 + Zfp978 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
12 NC_000070.6 4 147233360 + Zfp978 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
13 NC_000070.6 4 147363817 + Zfp978 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
14 NC_000070.6 4 147452723 + Zfp982 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
15 NC_000070.6 4 147495389 + Zfp982 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
16 NC_000070.6 4 147640000 - Zfp979 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
17 NC_000070.6 4 147806172 - Zfp984 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier II
18 NC_000084.6 18 36048398 + Nrg2 NNNTGACACTGCAATTTGAC NGG 2 0.3061 Tier II
19 NC_000069.6 3 95117272 - Vps72 NNNTGGCACTGCAATTTGGG NAG 2 0.1852 Tier II
20 NC_000078.6 12 71169346 + 2700049A03Rik NNNTGACACTGGAATTTGGG NAG 2 0.1152 Tier II
21 NC_000082.6 16 41755203 - Lsamp NNNTGACACTGCTATTTGGG NAG 2 0.0778 Tier II
22 NC_000072.6 6 92841313 - Adamts9 NNNTGACACTGGAAGTTGGG NGG 2 0.0222 Tier II
23 NC_000081.6 15 41503534 + Oxr1 NNNTGAGACTGCAATTTGGG NTG 2 0.0183 Tier II
24 NC_000081.6 15 83865891 - Gm41372 NNNTGACGCTGCATTTTGGG NGG 2 0.3911 Tier III
25 NC_000070.6 4 146513793 + Vmn2r-ps16 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier III
26 NC_000070.6 4 146614689 + LOC115486238 NNNTGTCACTGTAATTTGGG NGG 2 0.3846 Tier III
27 NC_000071.6 5 146589275 - Gm34333 NNNTGACACAGGAATTTGGG NGG 2 0.381 Tier III
28 NC_000067.6 1 119314807 + Gm33388 NNNTGACACTGCAATTTGAG NGA 2 0.0496 Tier III
29 NC_000072.6 6 92841313 - A730049H05Rik NNNTGACACTGGAAGTTGGG NGG 2 0.0222 Tier III
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Other clones with same target sequence:

(none)