Construct: sgRNA BRDN0001145653
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTATGATCGGATTATTGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK2 (4751)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76215
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
211674385 |
- |
NEK2 |
NNNATGATCGGATTATTGAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
50446162 |
+ |
SNTG1 |
NNNATGATTTGATTATTGAC |
NGG |
2 |
0.7109 |
Tier II |
| 3 |
NC_000020.11 |
20 |
25081229 |
- |
VSX1 |
NNNATGATCGAATGATTGAC |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000009.12 |
9 |
7078850 |
+ |
KDM4C |
NNNATGATTGGATGATTGAC |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000002.12 |
2 |
104138949 |
+ |
LOC105373523 |
NNNATTATTGGATTATTGAC |
NGG |
2 |
0.5833 |
Tier III |
| 6 |
NC_000002.12 |
2 |
131178722 |
- |
NEK2P4 |
NNNATGATCGTATTATTGAC |
NGG |
1 |
0.3846 |
Tier III |
| 7 |
NC_000014.9 |
14 |
18699754 |
- |
NEK2P3 |
NNNATGATCGTATTATTGAC |
NGG |
1 |
0.3846 |
Tier III |
| 8 |
NC_000014.9 |
14 |
19629231 |
+ |
LOC107984133 |
NNNATGATCGTATTATTGAC |
NGG |
1 |
0.3846 |
Tier III |
| 9 |
NC_000022.11 |
22 |
15612871 |
- |
NEK2P2 |
NNNATGATCGTATTATTGAC |
NGG |
1 |
0.3846 |
Tier III |
| 10 |
NC_000014.9 |
14 |
18890200 |
- |
NEK2P1 |
NNNATGATTGTATTATTGAC |
NGG |
2 |
0.3365 |
Tier III |
| 11 |
NC_000023.11 |
X |
22453237 |
- |
PTCHD1-AS |
NNNCTCATCGGATTATTGAC |
NGG |
2 |
0.2406 |
Tier III |
| 12 |
NC_000005.10 |
5 |
164485644 |
+ |
LOC102546299 |
NNNATGATCGGATTAAGGAC |
NGG |
2 |
0.107 |
Tier III |
| 13 |
NC_000005.10 |
5 |
164485644 |
+ |
LINC03000 |
NNNATGATCGGATTAAGGAC |
NGG |
2 |
0.107 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
191822276 |
+ |
Nek2 |
NNNATGATCGCATTATTGAC |
NGA |
2 |
0.0298 |
Tier I |
| 2 |
NC_000076.6 |
10 |
73842242 |
- |
Pcdh15 |
NNNATAATGGGATTATTGAC |
NGG |
2 |
0.619 |
Tier II |
| 3 |
NC_000077.6 |
11 |
81669821 |
- |
Asic2 |
NNNATGATTGGAATATTGAC |
NGG |
2 |
0.6058 |
Tier II |
| 4 |
NC_000075.6 |
9 |
107768736 |
- |
Rbm5 |
NNNATGATCTTATTATTGAC |
NGG |
2 |
0.3125 |
Tier II |
| 5 |
NC_000079.6 |
13 |
113345804 |
- |
Cspg4b |
NNNTTGATCGGATTATTGAC |
NAG |
2 |
0.165 |
Tier II |
| 6 |
NC_000072.6 |
6 |
36509472 |
- |
Chrm2 |
NNNATGACCGGATTATTGAC |
NTG |
2 |
0.039 |
Tier II |
Other clones with same target sequence:
(none)
