Construct: sgRNA BRDN0001145655
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACAGTAATACAGTCAACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PGM2L1 (283209)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77146
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
74374420 |
- |
PGM2L1 |
NNNAGTAATACAGTCAACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
43176487 |
- |
NBR1 |
NNNAGAAACACAGTCAACAC |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000007.14 |
7 |
101553302 |
- |
COL26A1 |
NNNAGTCACACAGTCAACAC |
NGG |
2 |
0.4038 |
Tier II |
| 4 |
NC_000007.14 |
7 |
66310671 |
- |
TPST1 |
NNNAGTAATACAGTCAAAAA |
NGG |
2 |
0.2692 |
Tier II |
| 5 |
NC_000001.11 |
1 |
236688533 |
- |
ACTN2 |
NNNAATAATACAGTCAACAT |
NGG |
2 |
0.26 |
Tier II |
| 6 |
NC_000016.10 |
16 |
83167555 |
- |
CDH13 |
NNNAGTAATAAAGCCAACAC |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000011.10 |
11 |
103308458 |
+ |
DYNC2H1 |
NNNAATAATACAGTGAACAC |
NGG |
2 |
0.0433 |
Tier II |
| 8 |
NC_000001.11 |
1 |
190124168 |
+ |
BRINP3 |
NNNATTAATACAGTCATCAC |
NGG |
2 |
0.04 |
Tier II |
| 9 |
NC_000001.11 |
1 |
155415515 |
+ |
ASH1L |
NNNAGTAGTACAGTGAACAC |
NGG |
2 |
0.0367 |
Tier II |
| 10 |
NC_000002.12 |
2 |
27156716 |
+ |
TCF23 |
NNNAGTAATACAGTGGACAC |
NGG |
2 |
0.0096 |
Tier II |
| 11 |
NC_000010.11 |
10 |
46019617 |
- |
NCOA4 |
NNNAGTAATACAGTCAGCAC |
NGT |
2 |
0.0028 |
Tier II |
| 12 |
NC_000021.9 |
21 |
42502183 |
+ |
SLC37A1 |
NNNAGTAATACAGTGAACAC |
NTG |
2 |
0.0019 |
Tier II |
| 13 |
NC_000020.11 |
20 |
7980187 |
- |
TMX4 |
NNNAGAAATACAGTCTACAC |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000018.10 |
18 |
58949146 |
- |
ZNF532 |
NNNAGTAAAACAGTCCACAC |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000006.12 |
6 |
14640241 |
- |
LOC101928354 |
NNNTGTAAAACAGTCAACAC |
NGG |
2 |
0.5909 |
Tier III |
| 16 |
NC_000008.11 |
8 |
89891251 |
+ |
LOC124901974 |
NNNTGCAATACAGTCAACAC |
NGG |
2 |
0.5785 |
Tier III |
| 17 |
NC_000001.11 |
1 |
48100571 |
+ |
LINC02794 |
NNNAGTAATTCAGTGAACAC |
NGG |
2 |
0.0441 |
Tier III |
| 18 |
NC_000003.12 |
3 |
129988847 |
+ |
RPS17P9 |
NNNAGTAATACAGTGAAGAC |
NGG |
2 |
0.0067 |
Tier III |
| 19 |
NC_000003.12 |
3 |
129988847 |
+ |
LOC124906284 |
NNNAGTAATACAGTGAAGAC |
NGG |
2 |
0.0067 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
100250455 |
+ |
Pgm2l1 |
NNNAGTAATACAGTCAACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
23671605 |
- |
Kcnma1 |
NNNAGTAACACAGTCAAAAC |
NGG |
2 |
0.497 |
Tier II |
| 3 |
NC_000085.6 |
19 |
57745016 |
- |
Atrnl1 |
NNNAGTAAAACAGTCAACAA |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000081.6 |
15 |
8971451 |
+ |
Ranbp3l |
NNNGGTAATACAGACAACAC |
NGG |
2 |
0.3869 |
Tier II |
| 5 |
NC_000067.6 |
1 |
188285710 |
- |
Ush2a |
NNNAGCAATACAGTCAACAC |
NAG |
2 |
0.2357 |
Tier II |
| 6 |
NC_000069.6 |
3 |
63368994 |
- |
Mme |
NNNAGTAACAGAGTCAACAC |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000084.6 |
18 |
43226563 |
- |
Stk32a |
NNNAGTAAAACAGTAAACAC |
NGG |
2 |
0.2063 |
Tier II |
| 8 |
NC_000076.6 |
10 |
64718170 |
- |
Ctnna3 |
NNNAGTAACACAGTCGACAC |
NGG |
2 |
0.1775 |
Tier II |
| 9 |
NC_000076.6 |
10 |
3917649 |
+ |
Plekhg1 |
NNNAGTAAGACAGCCAACAC |
NGG |
2 |
0.1769 |
Tier II |
| 10 |
NC_000078.6 |
12 |
84518200 |
- |
Lin52 |
NNNAGTTATATAGTCAACAC |
NGG |
2 |
0.1346 |
Tier II |
| 11 |
NC_000072.6 |
6 |
87184573 |
- |
Antxr1 |
NNNAGGAATACAGTCACCAC |
NGG |
2 |
0.1008 |
Tier II |
| 12 |
NC_000067.6 |
1 |
60150404 |
- |
Carf |
NNNAGTAATACAGCCAACAC |
NAG |
2 |
0.0741 |
Tier II |
| 13 |
NC_000086.7 |
X |
102399816 |
- |
Hdac8 |
NNNAGAAATACAGTCAACAC |
NGT |
2 |
0.014 |
Tier II |
| 14 |
NC_000076.6 |
10 |
13382196 |
+ |
Phactr2 |
NNNACTAATACAGTCAACAC |
NGT |
2 |
0.0127 |
Tier II |
| 15 |
NC_000076.6 |
10 |
89172176 |
+ |
Ano4 |
NNNAGTAATACAGTGAACCC |
NGG |
2 |
0.0103 |
Tier II |
| 16 |
NC_000070.6 |
4 |
77048051 |
+ |
Ptprd |
NNNAGTAAAACAGTCCACAC |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000077.6 |
11 |
37133189 |
+ |
Tenm2 |
NNNAGTAATACAGGTAACAC |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000083.6 |
17 |
35673185 |
- |
Gtf2h4 |
NNNAGTAATTCAGTCCACAC |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000080.6 |
14 |
48737892 |
- |
Otx2os1 |
NNNAGGAATACAGTCAACAC |
NGG |
1 |
0.5714 |
Tier III |
| 20 |
NC_000068.7 |
2 |
30777815 |
+ |
Gm39783 |
NNNAGTGATACAGCCAACAC |
NGG |
2 |
0.2017 |
Tier III |
| 21 |
NC_000080.6 |
14 |
104309685 |
- |
D130079A08Rik |
NNNAGTAATACATTCAAAAC |
NGG |
2 |
0.1615 |
Tier III |
| 22 |
NC_000080.6 |
14 |
19404270 |
+ |
Gm9637 |
NNNAGTAATATAGTCAACAC |
NCG |
2 |
0.033 |
Tier III |
| 23 |
NC_000086.7 |
X |
102399816 |
- |
Gm32262 |
NNNAGAAATACAGTCAACAC |
NGT |
2 |
0.014 |
Tier III |
| 24 |
NC_000067.6 |
1 |
78839577 |
- |
Gm29536 |
NNNATTAATACAGTCAACAC |
NGC |
2 |
0.0067 |
Tier III |
Other clones with same target sequence:
(none)
