Construct: sgRNA BRDN0001145661

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTTATCCCACAGAAAGACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK1 (3654)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76420

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 154014275 - IRAK1 NNNTATCCCACAGAAAGACC NGG 0 1.0 Tier I
2 NC_000007.14 7 117208336 - ST7 NNNTACTCCACAGAAAGACC NGG 2 0.7386 Tier II
3 NC_000013.11 13 33034144 - KL NNNTATCACACAGAAAAACC NGG 2 0.6067 Tier II
4 NC_000003.12 3 49002096 - P4HTM NNNGATCCCTCAGAAAGACC NGG 2 0.5515 Tier II
5 NC_000002.12 2 174186805 + OLA1 NNNTATAGCACAGAAAGACC NGG 2 0.4821 Tier II
6 NC_000003.12 3 65711715 + MAGI1 NNNTATCCAGCAGAAAGACC NGG 2 0.4762 Tier II
7 NC_000021.9 21 37780159 + KCNJ6 NNNTAAGCCACAGAAAGACC NGG 2 0.4078 Tier II
8 NC_000023.11 X 65007190 - ZC4H2 NNNTATCCCTCAGAAAGATC NGG 2 0.4072 Tier II
9 NC_000015.10 15 29791327 - TJP1 NNNTATCCCAAAGAAAGGCC NGG 2 0.3 Tier II
10 NC_000012.12 12 44584307 - NELL2 NNNTGTCCCACAGAAAGGCC NGG 2 0.288 Tier II
11 NC_000009.12 9 4357765 + GLIS3 NNNTATCCTACAGAAATACC NGG 2 0.2188 Tier II
12 NC_000002.12 2 177757536 + PDE11A NNNTATCCTACAGAAAGCCC NGG 2 0.1667 Tier II
13 NC_000023.11 X 28920047 - IL1RAPL1 NNNTATCCCCCAGAAAGATC NGG 2 0.1538 Tier II
14 NC_000002.12 2 2221948 + MYT1L NNNTTTCCCACAGAAAGGCC NGG 2 0.1455 Tier II
15 NC_000020.11 20 14570754 - MACROD2 NNNTCTCCCACAGAAGGACC NGG 2 0.0962 Tier II
16 NC_000022.11 22 48642701 - TAFA5 NNNTATCACACAGAAAGACC NCG 2 0.0696 Tier II
17 NC_000020.11 20 7908982 + HAO1 NNNTATCCCATAGCAAGACC NGG 2 0.0659 Tier II
18 NC_000005.10 5 59855541 + PDE4D NNNTATCCCACAGAAAAACC NGA 2 0.0648 Tier II
19 NC_000011.10 11 66282199 + CNIH2 NNNTATCCCACAGAAGGACT NGG 2 0.0577 Tier II
20 NC_000003.12 3 121656523 + HCLS1 NNNTATCCCACAGAAACCCC NGG 2 0.0448 Tier II
21 NC_000018.10 18 54882668 + RAB27B NNNTAACCCACAGAAAGACC NGC 2 0.0193 Tier II
22 NC_000004.12 4 177438260 + AGA NNNTATCCCACAGAAGGACC NTG 2 0.0075 Tier II
23 NC_000007.14 7 117208336 - ST7-OT3 NNNTACTCCACAGAAAGACC NGG 2 0.7386 Tier III
24 NC_000007.14 7 17460791 + LINC02888 NNNTAGCCCACAGAAAAACC NGG 2 0.5333 Tier III
25 NC_000007.14 7 17460791 + LOC105375172 NNNTAGCCCACAGAAAAACC NGG 2 0.5333 Tier III
26 NC_000002.12 2 174186805 + LOC124907906 NNNTATAGCACAGAAAGACC NGG 2 0.4821 Tier III
27 NC_000003.12 3 110460933 + LOC105374036 NNNTATCCTACAGAAAGAAC NGG 2 0.375 Tier III
28 NC_000019.10 19 44552357 + CEACAM22P NNNTATCCCACAGAAAAGCC NGG 2 0.3733 Tier III
29 NC_000012.12 12 115309160 - LOC124903082 NNNTCTCCCACAGAAAGACA NGG 2 0.25 Tier III
30 NC_000008.11 8 114952821 + LOC107986901 NNNTATCCCACTGAGAGACC NGG 2 0.2167 Tier III
31 NC_000020.11 20 14570754 - MACROD2-IT1 NNNTCTCCCACAGAAGGACC NGG 2 0.0962 Tier III
32 NC_000020.11 20 48057446 - LOC105372639 NNNTTTCCCACAGAAACACC NGG 2 0.0856 Tier III
33 NC_000020.11 20 48057446 - LOC124904923 NNNTTTCCCACAGAAACACC NGG 2 0.0856 Tier III
34 NC_000005.10 5 59855541 + LOC107986350 NNNTATCCCACAGAAAAACC NGA 2 0.0648 Tier III
35 NC_000008.11 8 2162976 + LOC105377781 NNNTATCCCACAGAAACACC NGT 2 0.0038 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 36900750 - Ppp2r2c NNNTATCCCAAAGAAAGACC NGG 1 0.75 Tier II
2 NC_000069.6 3 26836006 - Spata16 NNNTGTTCCACAGAAAGACC NGG 2 0.585 Tier II
3 NC_000074.6 8 61041375 - Nek1 NNNTATTACACAGAAAGACC NGG 2 0.5281 Tier II
4 NC_000086.7 X 161170339 + Scml2 NNNTATCCTACAGAAAGTCC NGG 2 0.4375 Tier II
5 NC_000070.6 4 134350927 + Slc30a2 NNNTTTTCCACAGAAAGACC NGG 2 0.2955 Tier II
6 NC_000081.6 15 38944901 - Baalc NNNTGTCCCACAGAAAGGCC NGG 2 0.288 Tier II
7 NC_000068.7 2 41672561 + Lrp1b NNNTGTCCCACTGAAAGACC NGG 2 0.24 Tier II
8 NC_000082.6 16 13890570 - Pdxdc1 NNNTATCTCACAGAAATACC NGG 2 0.2188 Tier II
9 NC_000081.6 15 73006809 + Trappc9 NNNAATCCCACAGAAAGAAC NGG 2 0.2041 Tier II
10 NC_000077.6 11 88431964 + Msi2 NNNTCTCCCACAGAAAGGCC NGG 2 0.2 Tier II
11 NC_000067.6 1 91819741 + Twist2 NNNTATCCCACAGAAAGCCA NGG 2 0.0952 Tier II
12 NC_000068.7 2 80161386 - Pde1a NNNTATCCCACAGAAAGCCA NGG 2 0.0952 Tier II
13 NC_000077.6 11 93284352 - Car10 NNNTATCCCACAGGAAGCCC NGG 2 0.0889 Tier II
14 NC_000071.6 5 76645401 - Cep135 NNNTATCCCACAGACAGACC NAG 2 0.0707 Tier II
15 NC_000076.6 10 99126880 - Poc1b NNNTATCCCACAGGAAGAGC NGG 2 0.0583 Tier II
16 NC_000080.6 14 25573467 + Zmiz1 NNNCATCCCACAGAAAGACC NGT 2 0.0104 Tier II
17 NC_000069.6 3 97596917 - Chd1l NNNTAGCCCACAGAAAGACC NGT 2 0.0092 Tier II
18 NC_000085.6 19 19042154 - Rorb NNNTATCCCACAGAATGCCC NGG 2 0.0 Tier II
19 NC_000067.6 1 102311018 + Gm3116 NNNGACCCCACAGAAAGACC NGG 2 0.5682 Tier III
20 NC_000078.6 12 112872127 - 1700127F24Rik NNNCATCCCCCAGAAAGACC NGG 2 0.2157 Tier III
21 NC_000070.6 4 89115638 - Gm12602 NNNTATCCCACAGAAATGCC NGG 2 0.1 Tier III
22 NC_000080.6 14 29741417 - Gm41118 NNNTACCCCACAGAAAGACC NCG 2 0.0974 Tier III
23 NC_000067.6 1 91819741 + Gm46117 NNNTATCCCACAGAAAGCCA NGG 2 0.0952 Tier III
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Other clones with same target sequence:

(none)