Construct: sgRNA BRDN0001145663
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATCAAGAACTTATCTACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBK1 (29110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76361
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
64484372 |
+ |
TBK1 |
NNNCAAGAACTTATCTACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
143600745 |
+ |
ARHGAP15 |
NNNAAAGAACTTGTCTACGA |
NGG |
2 |
0.5492 |
Tier II |
| 3 |
NC_000002.12 |
2 |
202159222 |
- |
KIAA2012 |
NNNCAAGAACTTATCTCCTA |
NGG |
2 |
0.1176 |
Tier II |
| 4 |
NC_000016.10 |
16 |
5710978 |
- |
RBFOX1 |
NNNCAAGAACTGATCTAGGA |
NGG |
2 |
0.0667 |
Tier II |
| 5 |
NC_000007.14 |
7 |
2082079 |
+ |
MAD1L1 |
NNNCAAGAACTTATGTAAGA |
NGG |
2 |
0.0269 |
Tier II |
| 6 |
NC_000002.12 |
2 |
143600745 |
+ |
LOC101928361 |
NNNAAAGAACTTGTCTACGA |
NGG |
2 |
0.5492 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
121561705 |
- |
Tbk1 |
NNNCAAGAACTTATCTACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
150121473 |
+ |
Zfp120 |
NNNCAATAACTTATCTACAA |
NGG |
2 |
0.4082 |
Tier II |
| 3 |
NC_000085.6 |
19 |
44809191 |
- |
Pax2 |
NNNCAAGAACTTGTCTAGGA |
NGG |
2 |
0.087 |
Tier II |
| 4 |
NC_000077.6 |
11 |
75180471 |
- |
Dph1 |
NNNCAAGAACTTATCTAGCA |
NGG |
2 |
0.0598 |
Tier II |
Other clones with same target sequence:
(none)
