Construct: sgRNA BRDN0001145664
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGATCGCTGGAACCTCCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKI (9162)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76614
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
137585265 |
- |
DGKI |
NNNTCGCTGGAACCTCCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
5683090 |
- |
EVC2 |
NNNTCGCTGAAGCCTCCATG |
NGG |
2 |
0.6741 |
Tier II |
| 3 |
NC_000010.11 |
10 |
7264518 |
+ |
SFMBT2 |
NNNTCCCTGTAACCTCCATG |
NGG |
2 |
0.554 |
Tier II |
| 4 |
NC_000007.14 |
7 |
105687235 |
- |
ATXN7L1 |
NNNTTGCAGGAACCTCCATG |
NGG |
2 |
0.5091 |
Tier II |
| 5 |
NC_000002.12 |
2 |
120782930 |
+ |
GLI2 |
NNNTCCCTGGGACCTCCATG |
NGG |
2 |
0.4432 |
Tier II |
| 6 |
NC_000008.11 |
8 |
58896206 |
- |
TOX |
NNNCCGCTTGAACCTCCATG |
NGG |
2 |
0.3451 |
Tier II |
| 7 |
NC_000018.10 |
18 |
58080518 |
+ |
NEDD4L |
NNNTAGCTGGAACCACCATG |
NGG |
2 |
0.3308 |
Tier II |
| 8 |
NC_000003.12 |
3 |
146422141 |
+ |
PLSCR2 |
NNNTGGCTGGCACCTCCATG |
NGG |
2 |
0.24 |
Tier II |
| 9 |
NC_000004.12 |
4 |
7359586 |
- |
SORCS2 |
NNNTCGTTGGAACCTCCACG |
NGG |
2 |
0.2241 |
Tier II |
| 10 |
NC_000019.10 |
19 |
33661517 |
- |
CHST8 |
NNNTCGATGGACCCTCCATG |
NGG |
2 |
0.1974 |
Tier II |
| 11 |
NC_000002.12 |
2 |
1818031 |
- |
MYT1L |
NNNTCGCTGGAACCTTCATC |
NGG |
2 |
0.1319 |
Tier II |
| 12 |
NC_000014.9 |
14 |
104724794 |
+ |
ADSS1 |
NNNACGCTGGACCCTCCATG |
NGG |
2 |
0.1253 |
Tier II |
| 13 |
NC_000017.11 |
17 |
33261410 |
- |
ASIC2 |
NNNTCCCTGGAAGCTCCATG |
NGG |
2 |
0.093 |
Tier II |
| 14 |
NC_000005.10 |
5 |
76292461 |
+ |
SV2C |
NNNTCTCTGGAAGCTCCATG |
NGG |
2 |
0.0909 |
Tier II |
| 15 |
NC_000008.11 |
8 |
144064625 |
- |
EXOSC4 |
NNNTCGCTGCAACCTCCATG |
NGA |
2 |
0.0278 |
Tier II |
| 16 |
NC_000003.12 |
3 |
160188572 |
+ |
IL12A-AS1 |
NNNTCTCTGGAACCTCCATG |
NGG |
1 |
0.6667 |
Tier III |
| 17 |
NC_000007.14 |
7 |
32827268 |
+ |
DPY19L1P2 |
NNNTCGCAGGAACTTCCATG |
NGG |
2 |
0.5867 |
Tier III |
| 18 |
NC_000014.9 |
14 |
48487646 |
- |
LOC105378178 |
NNNTCGCTTGAACCTCCAGG |
NGG |
2 |
0.1333 |
Tier III |
| 19 |
NC_000005.10 |
5 |
171499033 |
- |
LOC105377720 |
NNNTGGCTGGAAGCTCCATG |
NGG |
2 |
0.0818 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
37032919 |
- |
Dgki |
NNNTCGCTGGAACCTCCACG |
NAG |
2 |
0.0715 |
Tier I |
| 2 |
NC_000071.6 |
5 |
110396363 |
+ |
Fbrsl1 |
NNNTCCCAGGAACCTCCATG |
NGG |
2 |
0.5455 |
Tier II |
| 3 |
NC_000085.6 |
19 |
38040156 |
- |
Myof |
NNNTCCCTGGAACCCCCATG |
NGG |
2 |
0.186 |
Tier II |
| 4 |
NC_000067.6 |
1 |
158955244 |
- |
Pappa2 |
NNNTCGCTGGAACATCCTTG |
NGG |
2 |
0.175 |
Tier II |
| 5 |
NC_000077.6 |
11 |
29549923 |
+ |
Clhc1 |
NNNTTGCTGGAACCTCCATG |
NAG |
2 |
0.165 |
Tier II |
Other clones with same target sequence:
(none)
