Construct: sgRNA BRDN0001145668
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGAGGATGAGGACCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPEG (10290)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77624
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
219477735 |
+ |
SPEG |
NNNGAGGATGAGGACCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
69960992 |
+ |
CSN2 |
NNNGAGGATGAGGACCTTCA |
NGG |
2 |
0.125 |
Tier I |
| 3 |
NC_000009.12 |
9 |
138065392 |
+ |
CACNA1B |
NNNGAGGGTGAGTACCATCG |
NGG |
2 |
0.22 |
Tier II |
| 4 |
NC_000009.12 |
9 |
34959153 |
+ |
PHF24 |
NNNGAGGATGAGGACCAGTG |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000016.10 |
16 |
16079494 |
+ |
ABCC1 |
NNNTAGGATGAGGACCAGCG |
NGG |
2 |
0.1212 |
Tier II |
| 6 |
NC_000001.11 |
1 |
26827238 |
- |
ZDHHC18 |
NNNGAGGATGAGGACCAGGG |
NGG |
2 |
0.0417 |
Tier II |
| 7 |
NC_000012.12 |
12 |
124467558 |
- |
NCOR2 |
NNNGAGGATGAGGACGATGG |
NGG |
2 |
0.0192 |
Tier II |
| 8 |
NC_000012.12 |
12 |
124467050 |
- |
NCOR2 |
NNNGAGGATGAGGATCATGG |
NGG |
2 |
0.0083 |
Tier II |
| 9 |
NC_000019.10 |
19 |
47492603 |
+ |
NAPA |
NNNGTGGATGAGGACCATCG |
NGC |
2 |
0.0081 |
Tier II |
| 10 |
NC_000002.12 |
2 |
119747984 |
- |
LOC101927764 |
NNNAAGGATTAGGACCATCG |
NGG |
2 |
0.7313 |
Tier III |
| 11 |
NC_000020.11 |
20 |
6428324 |
- |
CASC20 |
NNNGAGGATGAGGAGCATAG |
NGG |
2 |
0.0214 |
Tier III |
| 12 |
NC_000019.10 |
19 |
47492603 |
+ |
NAPA-AS1 |
NNNGTGGATGAGGACCATCG |
NGC |
2 |
0.0081 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
75415366 |
+ |
Speg |
NNNGAGGATGAGGAACACCG |
NGG |
2 |
0.0952 |
Tier I |
| 2 |
NC_000075.6 |
9 |
56732541 |
- |
Lingo1 |
NNNGAGGATGAGGACAATCA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000072.6 |
6 |
83565800 |
+ |
Stambp |
NNNGAGGATGAGAACCATCT |
NGG |
2 |
0.6462 |
Tier II |
| 4 |
NC_000070.6 |
4 |
42900462 |
+ |
Phf24 |
NNNCAGGATGAGGACCATCA |
NGG |
2 |
0.4963 |
Tier II |
| 5 |
NC_000073.6 |
7 |
115798237 |
+ |
Sox6 |
NNNGAAGATGAGGACCATTG |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000070.6 |
4 |
154105519 |
- |
Trp73 |
NNNGAGGGTGAGGACCATAG |
NGG |
2 |
0.3143 |
Tier II |
| 7 |
NC_000076.6 |
10 |
14477087 |
- |
Adgrg6 |
NNNGAGGATGTGGACCATTG |
NGG |
2 |
0.142 |
Tier II |
| 8 |
NC_000081.6 |
15 |
71646342 |
+ |
Fam135b |
NNNGAGGATAAGGACCATGG |
NGG |
2 |
0.1167 |
Tier II |
| 9 |
NC_000068.7 |
2 |
126001674 |
- |
Fam227b |
NNNGAGGATGAGGACCTTTG |
NGG |
2 |
0.0615 |
Tier II |
| 10 |
NC_000073.6 |
7 |
91091554 |
+ |
Dlg2 |
NNNGAGGATGAGGAGCATCG |
NGG |
1 |
0.05 |
Tier II |
| 11 |
NC_000070.6 |
4 |
42900462 |
+ |
Gm52697 |
NNNCAGGATGAGGACCATCA |
NGG |
2 |
0.4963 |
Tier III |
| 12 |
NC_000076.6 |
10 |
95418988 |
- |
5730420D15Rik |
NNNGAGGATGAGGACCACTG |
NGG |
2 |
0.1978 |
Tier III |
| 13 |
NC_000073.6 |
7 |
136066469 |
+ |
Gm51502 |
NNNGAGGATGAGGCCCATCT |
NGG |
2 |
0.15 |
Tier III |
| 14 |
NC_000082.6 |
16 |
12645299 |
- |
Gm45919 |
NNNGAGGATGAGGACCTTCT |
NGG |
2 |
0.0933 |
Tier III |
| 15 |
NC_000084.6 |
18 |
4883003 |
+ |
Gm41659 |
NNNGAGGATGAGGAGCAGCG |
NGG |
2 |
0.0167 |
Tier III |
Other clones with same target sequence:
(none)
