Construct: sgRNA BRDN0001145669

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTTCCACCAAAGCCAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERKL (375298)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77676

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 181548783 + CERKL NNNTCCACCAAAGCCAAACA NGG 0 1.0 Tier I
2 NC_000006.12 6 16697775 + ATXN1 NNNTCTAACAAAGCCAAACA NGG 2 0.6036 Tier II
3 NC_000001.11 1 150932314 - SETDB1 NNNGCTACCAAAGCCAAACA NGG 2 0.5804 Tier II
4 NC_000011.10 11 70818695 - SHANK2 NNNCCCACCAGAGCCAAACA NGG 2 0.4206 Tier II
5 NC_000002.12 2 232319371 + DIS3L2 NNNTCCACAACAGCCAAACA NGG 2 0.3429 Tier II
6 NC_000002.12 2 215027023 - ABCA12 NNNTCAACCAATGCCAAACA NGG 2 0.3095 Tier II
7 NC_000009.12 9 37124494 - ZCCHC7 NNNATCACCAAAGCCAAACA NGG 2 0.303 Tier II
8 NC_000001.11 1 151201949 + PIP5K1A NNNTCCACCAAATCCAAACA NGG 1 0.3 Tier II
9 NC_000003.12 3 141947083 - TFDP2 NNNTCCACTCAAGCCAAACA NGG 2 0.2917 Tier II
10 NC_000004.12 4 95353021 + UNC5C NNNTCCACCAAAGCCAAATT NGG 2 0.2769 Tier II
11 NC_000020.11 20 9300510 - PLCB4 NNNTCCCCCGAAGCCAAACA NGG 2 0.2431 Tier II
12 NC_000015.10 15 48196220 - CTXN2 NNNTGCACCAAATCCAAACA NGG 2 0.18 Tier II
13 NC_000012.12 12 112595567 - RPH3A NNNTCCACCAATGCCAAAAA NGG 2 0.1429 Tier II
14 NC_000003.12 3 57030215 + ARHGEF3 NNNTCCACCAGAGCCACACA NGG 2 0.1147 Tier II
15 NC_000008.11 8 3705415 - CSMD1 NNNTTCACCAAAGCCAGACA NGG 2 0.1123 Tier II
16 NC_000002.12 2 124135347 + CNTNAP5 NNNTCCACCAAAGCCAGACA NAG 2 0.0458 Tier II
17 NC_000002.12 2 232645574 - EFHD1 NNNACCACCAAAGCCAAACA NGA 2 0.0331 Tier II
18 NC_000001.11 1 5991485 + NPHP4 NNNTCCACCAAAGCCACACA NCG 2 0.0189 Tier II
19 NC_000003.12 3 51714233 - GRM2 NNNTCCACCCAAGCCAAACA NGC 2 0.0074 Tier II
20 NC_000004.12 4 108095049 + LEF1 NNNTCCACCAAAGCCAAACA NCT 2 0.0 Tier II
21 NC_000017.11 17 37200561 - ACACA NNNTCCACCAAAGCCTTACA NGG 2 0.0 Tier II
22 NC_000003.12 3 70019868 - SAMMSON NNNTCAAACAAAGCCAAACA NGG 2 0.6036 Tier III
23 NC_000002.12 2 13583919 + NACAP9 NNNTCCAGTAAAGCCAAACA NGG 2 0.5625 Tier III
24 NC_000002.12 2 13583919 + LOC105373438 NNNTCCAGTAAAGCCAAACA NGG 2 0.5625 Tier III
25 NC_000002.12 2 84234945 + LOC107985905 NNNTCCATCAAAGCCAAACT NGG 2 0.525 Tier III
26 NC_000010.11 10 112354136 + GUCY2GP NNNTCCAACAAAGTCAAACA NGG 2 0.4767 Tier III
27 NC_000003.12 3 163371302 - LOC105374188 NNNTCCATCAAAGCCAAAAA NGG 2 0.375 Tier III
28 NC_000001.11 1 34579661 + LOC105378641 NNNTCCACCAAAGCCAAAAA NAG 2 0.1111 Tier III
29 NC_000017.11 17 70821590 + LOC105371884 NNNTCCACCAAAGCCAAATA NTG 2 0.018 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 79342406 + Cerkl NNNTCCACCAAAGCCAAACA NGG 0 1.0 Tier I
2 NC_000074.6 8 10562234 + Myo16 NNNTCCACCCAAGCCAAAGA NGG 2 0.0417 Tier I
3 NC_000076.6 10 31320197 - Hddc2 NNNTCTACAAAAGCCAAACA NGG 2 0.7959 Tier II
4 NC_000081.6 15 101845854 - Krt1 NNNTCCATCAAGGCCAAACA NGG 2 0.6319 Tier II
5 NC_000076.6 10 76846269 + Pcbp3 NNNTCAACCGAAGCCAAACA NGG 2 0.5159 Tier II
6 NC_000076.6 10 119872787 + Grip1 NNNTCCACTGAAGCCAAACA NGG 2 0.4861 Tier II
7 NC_000071.6 5 122297213 - Pptc7 NNNTCCACAGAAGCCAAACA NGG 2 0.4762 Tier II
8 NC_000081.6 15 32433037 + Sema5a NNNTCCTCCAAAGCCAAACA NGG 1 0.4375 Tier II
9 NC_000071.6 5 122393704 - Arpc3 NNNTCTTCCAAAGCCAAACA NGG 2 0.4062 Tier II
10 NC_000081.6 15 36091258 + Rgs22 NNNTACAACAAAGCCAAACA NGG 2 0.3714 Tier II
11 NC_000080.6 14 10671581 - Fhit NNNTCCACCAAAGCCAATCT NGG 2 0.3 Tier II
12 NC_000070.6 4 150378328 - Rere NNNCCCACCAAACCCAAACA NGG 2 0.2724 Tier II
13 NC_000067.6 1 158946085 + Pappa2 NNNGCCACCAAATCCAAACA NGG 2 0.1875 Tier II
14 NC_000071.6 5 116129291 - Ccdc60 NNNTCCACCAAGGCCAAACA NAG 2 0.1872 Tier II
15 NC_000067.6 1 71565698 + Atic NNNTCCACCAAAGTCAAACC NGG 2 0.1667 Tier II
16 NC_000069.6 3 105631148 - Kcnd3 NNNACCACCAATGCCAAACA NGG 2 0.1587 Tier II
17 NC_000071.6 5 37417872 - Evc2 NNNTCCAGCAAAGCCAGACA NGG 2 0.1134 Tier II
18 NC_000079.6 13 29206769 - Cdkal1 NNNTCCACCAATGCCAAACA NAG 2 0.0864 Tier II
19 NC_000073.6 7 79827554 + Anpep NNNTCCTCCAAAGCCACACA NGG 2 0.0772 Tier II
20 NC_000075.6 9 29665925 - Ntm NNNTCCTCCAAAGCCAGACA NGG 2 0.0772 Tier II
21 NC_000081.6 15 77802704 + Myh9 NNNTCCTCCAAAGCCAAAGA NGG 2 0.0547 Tier II
22 NC_000072.6 6 99418606 + Foxp1 NNNTCCACCAAATCCAGACA NGG 2 0.0529 Tier II
23 NC_000069.6 3 154063129 + Slc44a5 NNNCCCACCAAAGCTAAACA NGG 2 0.0431 Tier II
24 NC_000085.6 19 53380338 + Smndc1 NNNTCTACCAAAGCCAAACA NTG 2 0.0362 Tier II
25 NC_000083.6 17 56637539 - Catsperd NNNTACACCAAAGCCAAACA NTG 2 0.0223 Tier II
26 NC_000068.7 2 36219460 - Ptgs1 NNNTCCTCCAAAGCCAAACA NTG 2 0.017 Tier II
27 NC_000074.6 8 125455288 + Sipa1l2 NNNTCCACCAAAGCCAAACT NGC 2 0.0133 Tier II
28 NC_000076.6 10 6840094 - Oprm1 NNNTCCACCAAAGCCAGACA NGC 2 0.0039 Tier II
29 NC_000079.6 13 107655957 - Gm32186 NNNTCAACCAGAGCCAAACA NGG 2 0.6036 Tier III
30 NC_000070.6 4 106267058 - Gm12724 NNNTCCAACAAAACCAAACA NGG 2 0.6 Tier III
31 NC_000070.6 4 106267058 - Gm52671 NNNTCCAACAAAACCAAACA NGG 2 0.6 Tier III
32 NC_000080.6 14 24879159 - Gm41110 NNNCCCACCAAAACCAAACA NGG 2 0.5973 Tier III
33 NC_000076.6 10 99907586 - Gm35437 NNNTCCATCAGAGCCAAACA NGG 2 0.5688 Tier III
34 NC_000073.6 7 67213500 + 1700112J16Rik NNNCCCACCAAGGCCAAACA NGG 2 0.4673 Tier III
35 NC_000073.6 7 94642282 + Gm32647 NNNTCTACCAAAGCCAAACA NAG 2 0.2407 Tier III
36 NC_000078.6 12 111487624 - Gm40578 NNNTCCACCAAAGTCAAACC NGG 2 0.1667 Tier III
37 NC_000072.6 6 69946661 + Igk NNNTCCATCAAAGCCAGACA NGG 2 0.1544 Tier III
38 NC_000077.6 11 4296907 + Gm11958 NNNGCCACCAAAGCCAGACA NGG 2 0.1103 Tier III
39 NC_000076.6 10 42046932 - Gm40630 NNNTCCACCAAAGCCAGCCA NGG 2 0.0336 Tier III
40 NC_000076.6 10 42046932 - Gm51798 NNNTCCACCAAAGCCAGCCA NGG 2 0.0336 Tier III
41 NC_000069.6 3 122628237 + Gm43568 NNNTCCACCAAAGCCCAACT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)