Construct: sgRNA BRDN0001145671
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCCTAAAGGTATTCCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK2 (7444)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77233
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
58139758 |
- |
VRK2 |
NNNCCTAAAGGTATTCCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
8395048 |
+ |
RERE |
NNNCCCAAAGGCATTCCATG |
NGG |
2 |
0.8612 |
Tier II |
| 3 |
NC_000016.10 |
16 |
84075741 |
- |
MBTPS1 |
NNNCCTAAAGATATTCCATT |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000010.11 |
10 |
86865965 |
+ |
BMPR1A |
NNNCCTAAAAGGATTCCATG |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000007.14 |
7 |
123481208 |
- |
IQUB |
NNNCCTAAAGGAATACCATG |
NGG |
2 |
0.4632 |
Tier II |
| 6 |
NC_000011.10 |
11 |
12730457 |
+ |
TEAD1 |
NNNCCTAACGGTATTCCATT |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000019.10 |
19 |
19409958 |
- |
GATAD2A |
NNNCCTAAAGGTATTCTATT |
NGG |
2 |
0.3267 |
Tier II |
| 8 |
NC_000023.11 |
X |
71141999 |
- |
MED12 |
NNNCCTAAATTTATTCCATG |
NGG |
2 |
0.3125 |
Tier II |
| 9 |
NC_000023.11 |
X |
125025926 |
+ |
TENM1 |
NNNCCTAAAGGTATACAATG |
NGG |
2 |
0.2702 |
Tier II |
| 10 |
NC_000001.11 |
1 |
206545624 |
- |
RASSF5 |
NNNCCTAAAAGTATCCCATG |
NGG |
2 |
0.2545 |
Tier II |
| 11 |
NC_000018.10 |
18 |
229128 |
+ |
THOC1 |
NNNGCTAAAGGTATTCCATC |
NGG |
2 |
0.2143 |
Tier II |
| 12 |
NC_000019.10 |
19 |
8340051 |
- |
KANK3 |
NNNCCTGAAGGTTTTCCATG |
NGG |
2 |
0.2118 |
Tier II |
| 13 |
NC_000006.12 |
6 |
7798232 |
- |
BMP6 |
NNNCCTAAAGGTATACCCTG |
NGG |
2 |
0.1103 |
Tier II |
| 14 |
NC_000006.12 |
6 |
156881487 |
- |
ARID1B |
NNNCCTAAAGGTATTACATG |
NGA |
2 |
0.0694 |
Tier II |
| 15 |
NC_000001.11 |
1 |
92834316 |
- |
RPL5 |
NNNCCTAAAGGTCTTTCATG |
NGG |
2 |
0.0648 |
Tier II |
| 16 |
NC_000001.11 |
1 |
92834316 |
- |
DIPK1A |
NNNCCTAAAGGTCTTTCATG |
NGG |
2 |
0.0648 |
Tier II |
| 17 |
NC_000003.12 |
3 |
73068004 |
- |
PPP4R2 |
NNNCCTAAAGATATTCCATG |
NGC |
2 |
0.0222 |
Tier II |
| 18 |
NC_000006.12 |
6 |
55672893 |
- |
HMGCLL1 |
NNNCCTAAAGGTTTTCCATG |
NGA |
2 |
0.0208 |
Tier II |
| 19 |
NC_000003.12 |
3 |
1350266 |
- |
CNTN6 |
NNNCCTAAAGATATTCCATG |
NGT |
2 |
0.0161 |
Tier II |
| 20 |
NC_000003.12 |
3 |
187230800 |
+ |
MASP1 |
NNNCCAAAAGGTATTCCATG |
NGT |
2 |
0.014 |
Tier II |
| 21 |
NC_000002.12 |
2 |
29782635 |
- |
ALK |
NNNCCTAAAGGTATTCCCTG |
NGA |
2 |
0.0132 |
Tier II |
| 22 |
NC_000023.11 |
X |
139180416 |
+ |
FGF13 |
NNNCCTAAAGGTATTCCATG |
NAC |
2 |
0.0 |
Tier II |
| 23 |
NC_000023.11 |
X |
139180416 |
+ |
LOC124905222 |
NNNCCTAAAGGTATTCCATG |
NAC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
26483233 |
+ |
Vrk2 |
NNNCCTAAAGGTACTCCATC |
NGG |
2 |
0.1224 |
Tier I |
| 2 |
NC_000074.6 |
8 |
19446850 |
+ |
Defb8 |
NNNCTTAAGGGTATTCCATG |
NGG |
2 |
0.4242 |
Tier II |
| 3 |
NC_000078.6 |
12 |
35944535 |
- |
Agr3 |
NNNACTAAAGGTATTCAATG |
NGG |
2 |
0.393 |
Tier II |
| 4 |
NC_000085.6 |
19 |
37030146 |
- |
Cpeb3 |
NNNCCTAAAGATCTTCCATG |
NGG |
2 |
0.2105 |
Tier II |
| 5 |
NC_000068.7 |
2 |
65869895 |
- |
Csrnp3 |
NNNCCTAAAGGTTTTCCCTG |
NGG |
2 |
0.0571 |
Tier II |
| 6 |
NC_000075.6 |
9 |
34956094 |
+ |
Kirrel3 |
NNNCCTAAAGGAATTCCATG |
NGA |
2 |
0.0556 |
Tier II |
| 7 |
NC_000078.6 |
12 |
34747278 |
- |
Hdac9 |
NNNCCTAAAGGTAGTCCATC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000070.6 |
4 |
117050695 |
- |
Eif2b3 |
NNNTCTAAAGGTAGTCCATG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000079.6 |
13 |
38343773 |
- |
Gm47990 |
NNNCCTAAAGGTACTCCATT |
NGG |
2 |
0.2 |
Tier III |
| 10 |
NC_000075.6 |
9 |
34956094 |
+ |
Kirrel3os |
NNNCCTAAAGGAATTCCATG |
NGA |
2 |
0.0556 |
Tier III |
Other clones with same target sequence:
(none)
