Construct: sgRNA BRDN0001145672
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCCACGGCTCATCTAGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PASK (23178)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76203
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
241126989 |
+ |
PASK |
NNNCACGGCTCATCTAGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
125126157 |
+ |
SCAI |
NNNCAAGGCTCAACTAGAGT |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000001.11 |
1 |
206574138 |
- |
EIF2D |
NNNCACAGCTCAGCTAGAGT |
NGG |
2 |
0.2609 |
Tier II |
| 4 |
NC_000001.11 |
1 |
206574138 |
- |
RASSF5 |
NNNCACAGCTCAGCTAGAGT |
NGG |
2 |
0.2609 |
Tier II |
| 5 |
NC_000009.12 |
9 |
124325599 |
- |
NEK6 |
NNNCACGGCTCAGCTAGAGA |
NGG |
2 |
0.1467 |
Tier II |
| 6 |
NC_000017.11 |
17 |
45137296 |
+ |
ACBD4 |
NNNCACGGCTCATCACGAGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000007.14 |
7 |
3155973 |
+ |
LOC100129603 |
NNNCAGGGCTCATCTAAAGT |
NGG |
2 |
0.4667 |
Tier III |
| 8 |
NC_000008.11 |
8 |
100428067 |
- |
LOC124901990 |
NNNCACGGCTCATCTGGACT |
NGG |
2 |
0.0862 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
143949390 |
+ |
Pramel16 |
NNNCAAGGCTAATCTAGAGT |
NGG |
2 |
0.6964 |
Tier II |
| 2 |
NC_000070.6 |
4 |
144363218 |
- |
Pramel31 |
NNNCAAGGCTAATCTAGAGT |
NGG |
2 |
0.6964 |
Tier II |
| 3 |
NC_000082.6 |
16 |
94837315 |
+ |
Kcnj6 |
NNNCATGGCTTATCTAGAGT |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000085.6 |
19 |
38527097 |
+ |
Plce1 |
NNNCACAGCTCAGCTAGAGT |
NGG |
2 |
0.2609 |
Tier II |
| 5 |
NC_000075.6 |
9 |
65963522 |
+ |
Csnk1g1 |
NNNAACGGCTCAGCTAGAGT |
NGG |
2 |
0.2197 |
Tier II |
| 6 |
NC_000080.6 |
14 |
12698060 |
+ |
Cadps |
NNNCTCTGCTCATCTAGAGT |
NGG |
2 |
0.2078 |
Tier II |
| 7 |
NC_000081.6 |
15 |
74621265 |
- |
Mroh4 |
NNNCAGGGCTCATCTAGAGC |
NGG |
2 |
0.0455 |
Tier II |
| 8 |
NC_000078.6 |
12 |
53549287 |
+ |
Npas3 |
NNNCACGGCTCATCTAGAGT |
NTA |
2 |
0.0 |
Tier II |
| 9 |
NC_000070.6 |
4 |
143965326 |
+ |
Pramel28 |
NNNCAAGGCTAATCTAGAGT |
NGG |
2 |
0.6964 |
Tier III |
| 10 |
NC_000067.6 |
1 |
152233994 |
+ |
Gm36920 |
NNNCACTGATCATCTAGAGT |
NGG |
2 |
0.4898 |
Tier III |
Other clones with same target sequence:
(none)
