Construct: sgRNA BRDN0001145680
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGCTCTCGTACAGCAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1A1 (1452)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76189
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
149550137 |
+ |
CSNK1A1 |
NNNCTCTCGTACAGCAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
37105089 |
+ |
CSNK1A1L |
NNNCTCTCATACAGCAACTG |
NGG |
1 |
0.6429 |
Tier I |
| 3 |
NC_000001.11 |
1 |
36023257 |
- |
AGO3 |
NNNCTCTCTTGCAGCAACTG |
NGG |
2 |
0.3467 |
Tier II |
| 4 |
NC_000007.14 |
7 |
33174233 |
- |
BBS9 |
NNNCTCTCTCACAGCAACTG |
NGG |
2 |
0.2844 |
Tier II |
| 5 |
NC_000018.10 |
18 |
14128608 |
+ |
ZNF519 |
NNNCTCTCCTACAGAAACTG |
NGG |
2 |
0.1197 |
Tier II |
| 6 |
NC_000005.10 |
5 |
1280701 |
+ |
TERT |
NNNCTCTGGTACAGCCACTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000015.10 |
15 |
36817973 |
+ |
CSNK1A1P1 |
NNNCTCTTGTACAGCAACTG |
NGG |
1 |
0.875 |
Tier III |
| 8 |
NC_000011.10 |
11 |
102802931 |
+ |
WTAPP1 |
NNNCTCTCGTACGGCAACTG |
NGG |
1 |
0.6522 |
Tier III |
| 9 |
NC_000011.10 |
11 |
102807749 |
+ |
CSNK1A1P2 |
NNNCTTTCCTACAGCAACTG |
NGG |
2 |
0.5 |
Tier III |
| 10 |
NC_000011.10 |
11 |
102807749 |
+ |
WTAPP1 |
NNNCTTTCCTACAGCAACTG |
NGG |
2 |
0.5 |
Tier III |
| 11 |
NC_000003.12 |
3 |
125419530 |
+ |
LOC105374081 |
NNNCTCTCTCACAGCAACTG |
NGG |
2 |
0.2844 |
Tier III |
| 12 |
NC_000010.11 |
10 |
105673571 |
- |
LOC107984266 |
NNNCTCTCTCACAGCAACTG |
NGG |
2 |
0.2844 |
Tier III |
| 13 |
NC_000021.9 |
21 |
14031290 |
- |
LOC105377134 |
NNNCTCTCCTACAGAAACTG |
NGG |
2 |
0.1197 |
Tier III |
| 14 |
NC_000010.11 |
10 |
128306730 |
- |
LINC01163 |
NNNCTCTCGTACAGCCCCTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
61556548 |
- |
Csnk1a1 |
NNNCTCTCGTACAGCAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000086.7 |
X |
73707145 |
+ |
Bcap31 |
NNNCTCTCGTACAGCATCTA |
NGG |
2 |
0.125 |
Tier I |
| 3 |
NC_000080.6 |
14 |
70919536 |
- |
Gfra2 |
NNNCTCTCCTACAGCAACTT |
NGG |
2 |
0.3769 |
Tier II |
| 4 |
NC_000076.6 |
10 |
64071150 |
+ |
Lrrtm3 |
NNNCTGTCGTTCAGCAACTG |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000076.6 |
10 |
64071150 |
+ |
Ctnna3 |
NNNCTGTCGTTCAGCAACTG |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000073.6 |
7 |
135585022 |
- |
Ptpre |
NNNCTCTTGTACAGCAAGTG |
NGG |
2 |
0.1167 |
Tier II |
| 7 |
NC_000068.7 |
2 |
15385785 |
- |
Gm13366 |
NNNCTCTCATACAGCAACTG |
NGG |
1 |
0.6429 |
Tier III |
| 8 |
NC_000076.6 |
10 |
67419057 |
- |
Gm31962 |
NNNCTCACGTACAGCAAATG |
NGG |
2 |
0.4712 |
Tier III |
Other clones with same target sequence:
(none)
