Construct: sgRNA BRDN0001145682
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTGCAGCATTAACTAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CROT (54677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77709
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
87375700 |
+ |
CROT |
NNNTGCAGCATTAACTAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
87375700 |
+ |
ABCB4 |
NNNTGCAGCATTAACTAGTG |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000003.12 |
3 |
173456316 |
+ |
NLGN1 |
NNNGGCAGCATTTACTAGTG |
NGG |
2 |
0.1875 |
Tier II |
| 4 |
NC_000022.11 |
22 |
46408658 |
+ |
CELSR1 |
NNNTGCAGCTTTAACTGGTG |
NGG |
2 |
0.1557 |
Tier II |
| 5 |
NC_000016.10 |
16 |
10010673 |
+ |
GRIN2A |
NNNTGCAGCATTAACAAGTG |
NTG |
2 |
0.0354 |
Tier II |
| 6 |
NC_000023.11 |
X |
111031026 |
+ |
PAK3 |
NNNTGCAGCATGAACTAGTG |
NTG |
2 |
0.0195 |
Tier II |
| 7 |
NC_000003.12 |
3 |
190018747 |
- |
P3H2 |
NNNTGCAGCATTAAGTAGTG |
NAG |
2 |
0.013 |
Tier II |
| 8 |
NC_000006.12 |
6 |
131366149 |
+ |
LOC105378005 |
NNNAGAAGCATTAACTAGTG |
NGG |
2 |
0.4422 |
Tier III |
| 9 |
NC_000013.11 |
13 |
34510081 |
- |
LINC00457 |
NNNTGCAGCATTAAATAGTA |
NGG |
2 |
0.2083 |
Tier III |
| 10 |
NC_000013.11 |
13 |
34510081 |
- |
LINC02343 |
NNNTGCAGCATTAAATAGTA |
NGG |
2 |
0.2083 |
Tier III |
| 11 |
NC_000013.11 |
13 |
104022138 |
+ |
LOC124903203 |
NNNTGCTGCAGTAACTAGTG |
NGG |
2 |
0.175 |
Tier III |
| 12 |
NC_000015.10 |
15 |
36811296 |
- |
CSNK1A1P1 |
NNNTTCAGCATTAATTAGTG |
NGG |
2 |
0.02 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
106881724 |
+ |
Has3 |
NNNTCCAGCATCAACTAGTG |
NGG |
2 |
0.7444 |
Tier II |
| 2 |
NC_000083.6 |
17 |
50951954 |
+ |
Tbc1d5 |
NNNTGCAGTATTAACTACTG |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000075.6 |
9 |
117375280 |
- |
Rbms3 |
NNNTGCAGGATTAACTACTG |
NGG |
2 |
0.2948 |
Tier II |
| 4 |
NC_000085.6 |
19 |
32376467 |
- |
Sgms1 |
NNNTTCAGCTTTAACTAGTG |
NGG |
2 |
0.2647 |
Tier II |
| 5 |
NC_000068.7 |
2 |
84826600 |
- |
Smtnl1 |
NNNTGCAACATTAACTAGTG |
NAG |
2 |
0.2593 |
Tier II |
| 6 |
NC_000080.6 |
14 |
62956944 |
- |
Wdfy2 |
NNNTGCAGCATTTTCTAGTG |
NGG |
2 |
0.16 |
Tier II |
| 7 |
NC_000076.6 |
10 |
9523412 |
+ |
Samd5 |
NNNTGCATCATTAACTAGGG |
NGG |
2 |
0.1563 |
Tier II |
| 8 |
NC_000071.6 |
5 |
5318783 |
- |
Cdk14 |
NNNAGCAGCATTTACTAGTG |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000075.6 |
9 |
109784001 |
- |
Fbxw27 |
NNNTGCAGCATTACCTAGAG |
NGG |
2 |
0.0612 |
Tier II |
| 10 |
NC_000067.6 |
1 |
176933093 |
- |
Sdccag8 |
NNNTGCAGCATTAACTCGAG |
NGG |
2 |
0.0504 |
Tier II |
| 11 |
NC_000072.6 |
6 |
148867683 |
+ |
Caprin2 |
NNNTGCAGCACTAATTAGTG |
NGG |
2 |
0.0444 |
Tier II |
| 12 |
NC_000083.6 |
17 |
72017432 |
- |
Alk |
NNNTGCAGCATTAAGCAGTG |
NGG |
2 |
0.0333 |
Tier II |
| 13 |
NC_000079.6 |
13 |
24137601 |
+ |
Carmil1 |
NNNTGCAGCATTAACTATTG |
NGC |
2 |
0.0148 |
Tier II |
| 14 |
NC_000078.6 |
12 |
12477097 |
+ |
Gm34489 |
NNNTGAAGCATTAACTACTG |
NGG |
2 |
0.4422 |
Tier III |
| 15 |
NC_000067.6 |
1 |
176933093 |
- |
Gm15423 |
NNNTGCAGCATTAACTCGAG |
NGG |
2 |
0.0504 |
Tier III |
Other clones with same target sequence:
(none)
