Construct: sgRNA BRDN0001145686

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAAACAGGTGTCATCAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1L (138474)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77119

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 32633878 - TAF1L NNNAACAGGTGTCATCAGGG NGG 0 1.0 Tier I
2 NC_000023.11 X 71382647 + TAF1 NNNAACAGGAGTCATCAAGG NGG 2 0.5934 Tier I
3 NC_000017.11 17 34992667 - LIG3 NNNCACAGGTGTCATCAGCG NGG 2 0.1582 Tier I
4 NC_000022.11 22 24623486 + GGT1 NNNAACAGGAGTCATCAGGA NGG 2 0.8036 Tier II
5 NT_187386.1 22 81451 + LOC102724197 NNNAACAGGAGTCATCAGGA NGG 2 0.8036 Tier II
6 NC_000007.14 7 729446 + DNAAF5 NNNCACAGGTGTCATCAGGG NGG 1 0.3529 Tier II
7 NC_000001.11 1 213766732 - RPS6KC1 NNNAACAGGAGTAATCAGGG NGG 2 0.3297 Tier II
8 NC_000022.11 22 32786942 + SYN3 NNNACCAGGTTTCATCAGGG NGG 2 0.1923 Tier II
9 NC_000002.12 2 120737922 - GLI2 NNNAACTGGTCTCATCAGGG NGG 2 0.1875 Tier II
10 NC_000008.11 8 25981128 - EBF2 NNNATCAGGGGTCATCAGGG NGG 2 0.1818 Tier II
11 NC_000010.11 10 70845888 - SGPL1 NNNAACAGGTGTTATCAGGG NAG 2 0.1815 Tier II
12 NC_000019.10 19 35844699 + NPHS1 NNNAACAGGAGTCATGAGGG NGG 2 0.1319 Tier II
13 NC_000001.11 1 63924978 - ROR1 NNNAAAAGGTGTCATCTGGG NGG 2 0.1238 Tier II
14 NC_000005.10 5 11633969 - CTNND2 NNNAACAGATGTCATGAGGG NGG 2 0.0989 Tier II
15 NC_000021.9 21 37629103 + KCNJ6 NNNAACAGGTGTGTTCAGGG NGG 2 0.0727 Tier II
16 NC_000002.12 2 240909745 - CROCC2 NNNAACAGGTGTCAGCAGGG NGG 1 0.05 Tier II
17 NC_000016.10 16 596835 + RAB40C NNNAACAGATGTCAGCAGGG NGG 2 0.0321 Tier II
18 NC_000006.12 6 80305750 - BCKDHB NNNAACAGGTGTCATCAGTG NTG 2 0.026 Tier II
19 NC_000015.10 15 82674540 - AP3B2 NNNAACAGCTGTCATCAGGG NTG 2 0.021 Tier II
20 NC_000008.11 8 27764012 - CCDC25 NNNAGCAGGTGTCATCAGGG NGC 2 0.016 Tier II
21 NC_000020.11 20 15942259 - MACROD2 NNNAACAGGTGTCATCAGTG NGT 2 0.0108 Tier II
22 NC_000006.12 6 53275049 - ELOVL5 NNNAACAGGTGTGAGCAGGG NGG 2 0.0068 Tier II
23 NC_000022.11 22 18340546 + GGTLC5P NNNAACAGGAGTCATCAGGA NGG 2 0.8036 Tier III
24 NC_000022.11 22 18779161 - GGT3P NNNAACAGGAGTCATCAGGA NGG 2 0.8036 Tier III
25 NC_000022.11 22 21213496 - GGT2P NNNAACAGGAGTCATCAGGA NGG 2 0.8036 Tier III
26 NC_000006.12 6 18508396 + LOC105374955 NNNAAAAGGTGTCATCAAGG NGG 2 0.6429 Tier III
27 NC_000001.11 1 234647136 - LOC101927787 NNNAACAGGAGTCATCAGAG NGG 2 0.6122 Tier III
28 NC_000001.11 1 234647136 - LOC105373207 NNNAACAGGAGTCATCAGAG NGG 2 0.6122 Tier III
29 NC_000008.11 8 116283287 - LINC00536 NNNAAGAGGTGACATCAGGG NGG 2 0.4 Tier III
30 NC_000002.12 2 139465008 - LOC105373645 NNNTCCAGGTGTCATCAGGG NGG 2 0.3182 Tier III
31 NC_000012.12 12 131210265 + LINC01257 NNNAACAGGTGACCTCAGGG NGG 2 0.1714 Tier III
32 NC_000005.10 5 5126083 + RN7SKP73 NNNAACATGTGTCACCAGGG NGG 2 0.1705 Tier III
33 NC_000006.12 6 106773506 - LINC02532 NNNAGCAGGTGTCCTCAGGG NGG 2 0.1543 Tier III
34 NC_000006.12 6 53991953 - LOC101927189 NNNAACTGGTGTCACCAGGG NGG 2 0.1193 Tier III
35 NC_000021.9 21 37629103 + KCNJ6-AS1 NNNAACAGGTGTGTTCAGGG NGG 2 0.0727 Tier III
36 NC_000015.10 15 82674540 - CPEB1-AS1 NNNAACAGCTGTCATCAGGG NTG 2 0.021 Tier III
37 NC_000020.11 20 15942259 - LOC613266 NNNAACAGGTGTCATCAGTG NGT 2 0.0108 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 25585407 - Rabl6 NNNAACAGGTGTCATCAGAG NGT 2 0.0115 Tier I
2 NC_000067.6 1 151951709 + 1700025G04Rik NNNAACAGGTGACATCAGGG NGG 1 0.8 Tier II
3 NC_000073.6 7 43939742 - Klk15 NNNAACAGGTGACATCAGGA NGG 2 0.75 Tier II
4 NC_000072.6 6 80579672 + Lrrtm4 NNNAACAGATGTCATCAGGG NGG 1 0.6429 Tier II
5 NC_000068.7 2 24368060 - Psd4 NNNAACATGTGTCATCAGGG NGG 1 0.625 Tier II
6 NC_000083.6 17 13716719 + Tcte2 NNNAACAGGTGTCATCAGCA NGG 2 0.4203 Tier II
7 NC_000068.7 2 65014401 - Grb14 NNNAACAGTTGTCATCAAGG NGG 2 0.3692 Tier II
8 NC_000068.7 2 66195250 + Ttc21b NNNAACAGGTGGTATCAGGG NGG 2 0.35 Tier II
9 NC_000080.6 14 64000185 - Rp1l1 NNNAACATCTGTCATCAGGG NGG 2 0.3365 Tier II
10 NC_000080.6 14 24456229 + Polr3a NNNAACAAGTGTCATCAGGG NAG 2 0.2593 Tier II
11 NC_000077.6 11 84400614 - Acaca NNNCACAGGTGTCATCAGGT NGG 2 0.2471 Tier II
12 NC_000072.6 6 53063430 + Jazf1 NNNAACAGTTGTCATCAGGC NGG 2 0.2286 Tier II
13 NC_000073.6 7 44006733 - Klk1b21 NNNAACAGGGGTAATCAGGG NGG 2 0.1923 Tier II
14 NC_000081.6 15 79642392 + Fam227a NNNAACAGGTGTCATCGGGA NGG 2 0.1654 Tier II
15 NC_000071.6 5 137337353 + Slc12a9 NNNAGCAGGTGTCCTCAGGG NGG 2 0.1543 Tier II
16 NC_000082.6 16 33754478 + Heg1 NNNAACAGGTGTCATCCGAG NGG 2 0.1261 Tier II
17 NC_000078.6 12 111409414 - Lbhd2 NNNAACAGGTGTCACCAGGC NGG 2 0.1169 Tier II
18 NC_000080.6 14 24783493 - Gm31838 NNNAACAGGTGACATCATGG NGG 2 0.5333 Tier III
19 NC_000078.6 12 10159166 - Gm5182 NNNCACAGGTGTCATCAGGT NGG 2 0.2471 Tier III
20 NC_000072.6 6 53063430 + Gm33097 NNNAACAGTTGTCATCAGGC NGG 2 0.2286 Tier III
21 NC_000073.6 7 44006733 - Klk1b12-ps NNNAACAGGGGTAATCAGGG NGG 2 0.1923 Tier III
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Other clones with same target sequence:

(none)