Construct: sgRNA BRDN0001145690
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTGATTTAGCGTCTAGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMPR1B (658)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77488
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
95131323 |
- |
BMPR1B |
NNNTGATTTAGCGTCTAGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
73839990 |
- |
CAMK2G |
NNNTCATTTAGCTTCTAGGG |
NGG |
2 |
0.2357 |
Tier II |
| 3 |
NC_000015.10 |
15 |
85563156 |
+ |
AKAP13 |
NNNTGATTTAGCCTCTAGGG |
NAG |
2 |
0.1092 |
Tier II |
| 4 |
NC_000006.12 |
6 |
1014580 |
- |
LINC01622 |
NNNTGATTTGGCATCTAGGG |
NGG |
2 |
0.5128 |
Tier III |
| 5 |
NC_000001.11 |
1 |
185370600 |
+ |
CBSLR |
NNNTGATTTAGCATCTAGGG |
NAG |
2 |
0.2393 |
Tier III |
| 6 |
NC_000015.10 |
15 |
83365514 |
- |
LOC105370934 |
NNNTGATTTAGCGTCTGGGG |
NAG |
2 |
0.0458 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
81054770 |
+ |
Smoc1 |
NNNTGATTGAGCATCTAGGG |
NGG |
2 |
0.5714 |
Tier II |
| 2 |
NC_000080.6 |
14 |
122971276 |
+ |
Tmtc4 |
NNNTGATTTAGAGTCTAGTG |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000067.6 |
1 |
173402341 |
+ |
Aim2 |
NNNTGATTTAGTGTCTAGTG |
NGG |
2 |
0.359 |
Tier II |
Other clones with same target sequence:
(none)
