Construct: sgRNA BRDN0001145691
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAACAATCGGAATCATCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TLK1 (9874)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75994
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
171061129 |
- |
TLK1 |
NNNACAATCGGAATCATCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
37445992 |
- |
NWD2 |
NNNACAATCGGATCCATCCA |
NGG |
2 |
0.0857 |
Tier I |
| 3 |
NC_000010.11 |
10 |
53811613 |
- |
PCDH15 |
NNNACAATAGGAATTATCCA |
NGG |
2 |
0.0571 |
Tier I |
| 4 |
NC_000007.14 |
7 |
150373870 |
- |
REPIN1 |
NNNACAATTAGAATCATCCA |
NGG |
2 |
0.8167 |
Tier II |
| 5 |
NC_000001.11 |
1 |
25885991 |
+ |
STMN1 |
NNNACAAATGGAATCATCCA |
NGG |
2 |
0.7 |
Tier II |
| 6 |
NC_000005.10 |
5 |
1433221 |
+ |
SLC6A3 |
NNNACCATCAGAATCATCCA |
NGG |
2 |
0.4242 |
Tier II |
| 7 |
NC_000009.12 |
9 |
122686470 |
+ |
TLK1P1 |
NNNACAATCAGAATCATCCA |
NGG |
1 |
0.9333 |
Tier III |
| 8 |
NC_000015.10 |
15 |
87544978 |
+ |
LOC102724465 |
NNNACAATCGGAATCATCCA |
NAC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
70757522 |
- |
Tlk1 |
NNNACAATCAGAGTCATCCA |
NGG |
2 |
0.6087 |
Tier I |
Other clones with same target sequence:
(none)
