Construct: sgRNA BRDN0001145692
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTGCAGGTCGTCACGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK6 (1021)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75643
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
92671527 |
- |
CDK6 |
NNNTGCAGGTCGTCACGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
75816781 |
- |
UNK |
NNNTGCAGGTCGTCACTCAG |
NGG |
2 |
0.0714 |
Tier I |
| 3 |
NC_000023.11 |
X |
64348429 |
+ |
MTMR8 |
NNNTGCAGGACGTCACACTG |
NGG |
2 |
0.8 |
Tier II |
| 4 |
NC_000003.12 |
3 |
185498586 |
- |
TMEM41A |
NNNTGCAGGGCGTCACGCCG |
NGG |
2 |
0.1379 |
Tier II |
| 5 |
NC_000002.12 |
2 |
97664646 |
+ |
C2orf92 |
NNNTGCAAGTCGTCACGGTG |
NGG |
2 |
0.1333 |
Tier II |
| 6 |
NC_000008.11 |
8 |
47731598 |
- |
SPIDR |
NNNTGCAGGTGGTCACTCTG |
NGG |
2 |
0.0625 |
Tier II |
| 7 |
NC_000016.10 |
16 |
82982707 |
+ |
CDH13 |
NNNTGCAGGTCGTCTCCCTG |
NGG |
2 |
0.0471 |
Tier II |
| 8 |
NC_000022.11 |
22 |
12693877 |
+ |
DUX4L44 |
NNNAGCAGGCCGTCACGCTG |
NGG |
2 |
0.254 |
Tier III |
| 9 |
NC_000009.12 |
9 |
85136918 |
+ |
LOC107987088 |
NNNTGCAGATTGTCACGCTG |
NGG |
2 |
0.1978 |
Tier III |
| 10 |
NC_000002.12 |
2 |
97664646 |
+ |
RNU4-8P |
NNNTGCAAGTCGTCACGGTG |
NGG |
2 |
0.1333 |
Tier III |
| 11 |
NC_000002.12 |
2 |
97664646 |
+ |
LOC124900516 |
NNNTGCAAGTCGTCACGGTG |
NGG |
2 |
0.1333 |
Tier III |
| 12 |
NC_000016.10 |
16 |
82982707 |
+ |
LOC101928417 |
NNNTGCAGGTCGTCTCCCTG |
NGG |
2 |
0.0471 |
Tier III |
| 13 |
NC_000021.9 |
21 |
45586212 |
- |
LOC107985485 |
NNNTGCAGGTCTTCACGCTG |
NGA |
2 |
0.0267 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
3473128 |
+ |
Cdk6 |
NNNTGCAGGTCGTCACGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
24420581 |
- |
Cdk5 |
NNNTCCAGGTGGTCACGCTG |
NGG |
2 |
0.1964 |
Tier I |
| 3 |
NC_000072.6 |
6 |
127617851 |
+ |
Cracr2a |
NNNTGAAGGTCCTCACGCTG |
NGG |
2 |
0.4916 |
Tier II |
| 4 |
NC_000071.6 |
5 |
121341242 |
- |
Hectd4 |
NNNTGCAGCTCCTCACGCTG |
NGG |
2 |
0.2851 |
Tier II |
| 5 |
NC_000069.6 |
3 |
40655022 |
- |
Intu |
NNNTGGAGGCCGTCACGCTG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000068.7 |
2 |
144765088 |
+ |
Dtd1 |
NNNTGCAGGTCCTCATGCTG |
NGG |
2 |
0.1629 |
Tier II |
| 7 |
NC_000078.6 |
12 |
54981397 |
+ |
Baz1a |
NNNTGCAGGTCCTCATGCTG |
NGG |
2 |
0.1629 |
Tier II |
| 8 |
NC_000074.6 |
8 |
124717119 |
+ |
Ttc13 |
NNNTGCAGGTCATCAGGCTG |
NGG |
2 |
0.1436 |
Tier II |
| 9 |
NC_000078.6 |
12 |
54981397 |
+ |
Gm51983 |
NNNTGCAGGTCCTCATGCTG |
NGG |
2 |
0.1629 |
Tier III |
| 10 |
NC_000071.6 |
5 |
140858973 |
- |
Gm30804 |
NNNTGCAGGTCCTCAGGCTG |
NGG |
2 |
0.0814 |
Tier III |
| 11 |
NC_000072.6 |
6 |
145567629 |
- |
Gm38475 |
NNNTGCAGTTCGTCACGGTG |
NGG |
2 |
0.0711 |
Tier III |
| 12 |
NC_000077.6 |
11 |
54204858 |
+ |
4933405E24Rik |
NNNTGCAGGTCCTCACGCTG |
NTG |
2 |
0.0206 |
Tier III |
| 13 |
NC_000077.6 |
11 |
54204858 |
+ |
Gm12223 |
NNNTGCAGGTCCTCACGCTG |
NTG |
2 |
0.0206 |
Tier III |
Other clones with same target sequence:
(none)
