Construct: sgRNA BRDN0001145704
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGAAGCAGAGTTTATCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- GRK3 (157)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000022.11 | 22 | 25685216 | - | GRK3 | NNNGAAGCAGAGTTTATCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000007.14 | 7 | 30617193 | - | GARS1 | NNNGAAGCGGAGTTTATCTG | NAG | 2 | 0.1728 | Tier I |
3 | NC_000023.11 | X | 136774988 | + | ARHGEF6 | NNNGAAGCAAAGTTTATCTG | NGG | 1 | 0.9333 | Tier II |
4 | NC_000018.10 | 18 | 27101078 | - | CHST9 | NNNGAACAAGAGTTTATCTG | NGG | 2 | 0.4469 | Tier II |
5 | NC_000005.10 | 5 | 149632708 | + | ARHGEF37 | NNNGAAGCTGAGTTTATCTT | NGG | 2 | 0.42 | Tier II |
6 | NC_000009.12 | 9 | 8605123 | - | PTPRD | NNNGAAGAAGAGTTTATTTG | NGG | 2 | 0.4179 | Tier II |
7 | NC_000008.11 | 8 | 62643665 | - | NKAIN3 | NNNGAAGCAGGGTTTATTTG | NGG | 2 | 0.4179 | Tier II |
8 | NC_000011.10 | 11 | 124772264 | - | MSANTD2 | NNNGAACCTGAGTTTATCTG | NGG | 2 | 0.4125 | Tier II |
9 | NC_000007.14 | 7 | 147011617 | + | CNTNAP2 | NNNGAAGCAAAGTTTATCTC | NGG | 2 | 0.4 | Tier II |
10 | NC_000007.14 | 7 | 34931996 | - | DPY19L1 | NNNGAGGCAGAGTTTAACTG | NGG | 2 | 0.381 | Tier II |
11 | NC_000002.12 | 2 | 33357353 | + | LTBP1 | NNNGCAGCAGAGATTATCTG | NGG | 2 | 0.3462 | Tier II |
12 | NC_000005.10 | 5 | 22271065 | + | CDH12 | NNNTAAGCAGAATTTATCTG | NGG | 2 | 0.3394 | Tier II |
13 | NC_000017.11 | 17 | 73591624 | + | SDK2 | NNNGAAGCAATGTTTATCTG | NGG | 2 | 0.2872 | Tier II |
14 | NC_000007.14 | 7 | 28160148 | + | JAZF1 | NNNGATGCACAGTTTATCTG | NGG | 2 | 0.2857 | Tier II |
15 | NC_000015.10 | 15 | 47405712 | - | SEMA6D | NNNGCAGCAGAGTTTATATG | NGG | 2 | 0.2692 | Tier II |
16 | NC_000010.11 | 10 | 28102786 | - | MPP7 | NNNGAAGCAGATATTATCTG | NGG | 2 | 0.2663 | Tier II |
17 | NC_000005.10 | 5 | 151370215 | + | SLC36A1 | NNNGAAGCAGATTATATCTG | NGG | 2 | 0.2381 | Tier II |
18 | NC_000004.12 | 4 | 139517797 | + | SETD7 | NNNGAAGCAGCCTTTATCTG | NGG | 2 | 0.2118 | Tier II |
19 | NC_000006.12 | 6 | 167939523 | - | AFDN | NNNTCAGCAGAGTTTATCTG | NGG | 2 | 0.1818 | Tier II |
20 | NC_000016.10 | 16 | 70554252 | + | SF3B3 | NNNGAAGCAGAGTATATCTG | NAG | 2 | 0.1605 | Tier II |
21 | NC_000012.12 | 12 | 69908523 | + | MYRFL | NNNGGAGCAGAGTTTGTCTG | NGG | 2 | 0.1385 | Tier II |
22 | NC_000009.12 | 9 | 121599243 | + | DAB2IP | NNNGAAGCAGAGGTTATCTC | NGG | 2 | 0.1118 | Tier II |
23 | NC_000008.11 | 8 | 40863084 | + | ZMAT4 | NNNGAAGCAGAGGTCATCTG | NGG | 2 | 0.0711 | Tier II |
24 | NC_000008.11 | 8 | 143876588 | - | EPPK1 | NNNGAAGCAGAGTCTGTCTG | NGG | 2 | 0.0549 | Tier II |
25 | NC_000011.10 | 11 | 64990300 | - | BATF2 | NNNGAAGCAGAGTTCATGTG | NGG | 2 | 0.0364 | Tier II |
26 | NC_000012.12 | 12 | 120986241 | - | HNF1A | NNNGGAGCAGAGTTTATCTG | NTG | 2 | 0.0281 | Tier II |
27 | NC_000022.11 | 22 | 19087001 | - | DGCR2 | NNNGAAGCAGAGTTTATTTG | NTG | 2 | 0.025 | Tier II |
28 | NC_000015.10 | 15 | 64618686 | - | ZNF609 | NNNGAAGCAGAGTTTGGCTG | NGG | 2 | 0.0226 | Tier II |
29 | NC_000011.10 | 11 | 45879331 | - | CRY2 | NNNGAAGCAGATTTTATCTG | NTG | 2 | 0.015 | Tier II |
30 | NC_000015.10 | 15 | 42510965 | + | SNAP23 | NNNGAACCAGAGTTTATCTG | NGT | 2 | 0.0111 | Tier II |
31 | NC_000010.11 | 10 | 113571671 | + | HABP2 | NNNGAAGCAGAGTTTCACTG | NGG | 2 | 0.0 | Tier II |
32 | NC_000002.12 | 2 | 166531614 | - | LOC124906087 | NNNAAAGCAGAGTTTATCTA | NGG | 2 | 0.8438 | Tier III |
33 | NC_000005.10 | 5 | 89282168 | + | MEF2C-AS1 | NNNGATGCAAAGTTTATCTG | NGG | 2 | 0.6667 | Tier III |
34 | NC_000020.11 | 20 | 38425061 | - | SNHG17 | NNNGAAACAGGGTTTATCTG | NGG | 2 | 0.65 | Tier III |
35 | NC_000002.12 | 2 | 67115951 | + | LINC01828 | NNNCAAACAGAGTTTATCTG | NGG | 2 | 0.5294 | Tier III |
36 | NC_000005.10 | 5 | 151370215 | + | LOC105378234 | NNNGAAGCAGATTATATCTG | NGG | 2 | 0.2381 | Tier III |
37 | NC_000013.11 | 13 | 78098182 | + | OBI1-AS1 | NNNCAAGCAGAGTTTATCTC | NGG | 2 | 0.2269 | Tier III |
38 | NC_000005.10 | 5 | 88378428 | + | TMEM161B-DT | NNNGAAGCAGAATTTATCTG | NTG | 2 | 0.0364 | Tier III |
39 | NC_000001.11 | 1 | 219792600 | + | LOC105372926 | NNNAAAGCAGAGTTTATCTG | NTG | 2 | 0.0351 | Tier III |
40 | NC_000015.10 | 15 | 64618686 | - | LOC101930091 | NNNGAAGCAGAGTTTGGCTG | NGG | 2 | 0.0226 | Tier III |
41 | NC_000004.12 | 4 | 104607990 | + | CXXC4-AS1 | NNNAAAGCAGAGTTTATCTG | NGT | 2 | 0.0145 | Tier III |
42 | NC_000004.12 | 4 | 104607990 | + | LOC124900745 | NNNAAAGCAGAGTTTATCTG | NGT | 2 | 0.0145 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000074.6 | 8 | 61876954 | - | Palld | NNNGAAGCAGAGTTTATTTG | NAG | 2 | 0.1667 | Tier I |
2 | NC_000082.6 | 16 | 14149077 | - | Marf1 | NNNGAAGCAGAGTTTATCTG | NGT | 1 | 0.0161 | Tier I |
3 | NC_000072.6 | 6 | 124828160 | + | Usp5 | NNNGAAGCAGAGCTTATCTG | NGG | 1 | 0.7895 | Tier II |
4 | NC_000075.6 | 9 | 106668660 | - | Tex264 | NNNGAAGCAGAACTTATCTG | NGG | 2 | 0.7368 | Tier II |
5 | NC_000076.6 | 10 | 18686960 | - | Arfgef3 | NNNGATGCAGAGATTATCTG | NGG | 2 | 0.4945 | Tier II |
6 | NC_000069.6 | 3 | 27143235 | + | Ect2 | NNNGAACAAGAGTTTATCTG | NGG | 2 | 0.4469 | Tier II |
7 | NC_000079.6 | 13 | 115049882 | - | Itga1 | NNNGAAGCAGAATTTATCTC | NGG | 2 | 0.4 | Tier II |
8 | NC_000074.6 | 8 | 82958448 | + | Rnf150 | NNNCATGCAGAGTTTATCTG | NGG | 2 | 0.3782 | Tier II |
9 | NC_000077.6 | 11 | 75349525 | - | Smyd4 | NNNGAAGGAGAGTTAATCTG | NGG | 2 | 0.3722 | Tier II |
10 | NC_000075.6 | 9 | 79910791 | - | Filip1 | NNNGCAGAAGAGTTTATCTG | NGG | 2 | 0.325 | Tier II |
11 | NC_000081.6 | 15 | 35646736 | - | Vps13b | NNNGAAGCAATGTTTATCTG | NGG | 2 | 0.2872 | Tier II |
12 | NC_000074.6 | 8 | 126322464 | + | Slc35f3 | NNNGAACCAGATTTTATCTG | NGG | 2 | 0.2644 | Tier II |
13 | NC_000067.6 | 1 | 67147235 | - | Cps1 | NNNAAAGCAGAGTTCATCTG | NGG | 2 | 0.2455 | Tier II |
14 | NC_000082.6 | 16 | 10742873 | - | Clec16a | NNNGAAGCAGATTATATCTG | NGG | 2 | 0.2381 | Tier II |
15 | NC_000067.6 | 1 | 88324932 | + | Trpm8 | NNNGAAGAAGAGTTTATCTG | NAG | 2 | 0.1685 | Tier II |
16 | NC_000067.6 | 1 | 170323473 | + | Nos1ap | NNNGAAGCAGAGTTTATATG | NAG | 2 | 0.1396 | Tier II |
17 | NC_000067.6 | 1 | 167719634 | - | Lmx1a | NNNGAAGAAGAGTTTGTCTG | NGG | 2 | 0.125 | Tier II |
18 | NC_000071.6 | 5 | 74699634 | - | Lnx1 | NNNGATGCAGAGTTTATGTG | NGG | 2 | 0.0952 | Tier II |
19 | NC_000077.6 | 11 | 5761701 | + | Urgcp | NNNGAAGCAGAGTTTAGCTT | NGG | 2 | 0.0824 | Tier II |
20 | NC_000071.6 | 5 | 115062052 | + | Sppl3 | NNNGAAGCAGAGTTTATCTG | NGA | 1 | 0.0694 | Tier II |
21 | NC_000082.6 | 16 | 17328543 | + | Pi4ka | NNNGAACCAGAGTTTATCTG | NGA | 2 | 0.0477 | Tier II |
22 | NC_000068.7 | 2 | 120830317 | - | Ttbk2 | NNNGAAGCAGAGCTGATCTG | NGG | 2 | 0.0395 | Tier II |
23 | NC_000073.6 | 7 | 57779377 | + | Gabrb3 | NNNGAAGCAGAGTAGATCTG | NGG | 2 | 0.031 | Tier II |
24 | NC_000075.6 | 9 | 56115735 | - | Pstpip1 | NNNGCAGCAGAGTTTATCTG | NTG | 2 | 0.0195 | Tier II |
25 | NC_000068.7 | 2 | 92203284 | + | Phf21a | NNNGAAACAGAGTTTATCTG | NGT | 2 | 0.0161 | Tier II |
26 | NC_000071.6 | 5 | 100865312 | + | Gpat3 | NNNGAAGAAGAGTTTATCTG | NGC | 2 | 0.0144 | Tier II |
27 | NC_000079.6 | 13 | 59093417 | - | Ntrk2 | NNNGAAGCAGACTTTATCTG | NGC | 2 | 0.0118 | Tier II |
28 | NC_000086.7 | X | 9280347 | - | Xk | NNNGAAGCAGAGTATATCTG | NGT | 2 | 0.01 | Tier II |
29 | NC_000085.6 | 19 | 22183761 | - | Trpm3 | NNNGAAGCAGAGTTTGTCTG | NGT | 2 | 0.0031 | Tier II |
30 | NC_000071.6 | 5 | 130415164 | + | Caln1 | NNNGAAGCAGAGTTTATGTG | NGT | 2 | 0.0022 | Tier II |
31 | NC_000078.6 | 12 | 99748640 | - | Efcab11 | NNNCAAGCAGAGTTTCTCTG | NGG | 2 | 0.0 | Tier II |
32 | NC_000084.6 | 18 | 11676761 | + | Rbbp8 | NNNGAAACAGAGTTTTTCTG | NGG | 2 | 0.0 | Tier II |
33 | NC_000075.6 | 9 | 116026720 | - | Gadl1 | NNNGAAGCAGAATTTCTCTG | NGG | 2 | 0.0 | Tier II |
34 | NC_000072.6 | 6 | 118883098 | + | Cacna1c | NNNGAAGCAGAGCTTCTCTG | NGG | 2 | 0.0 | Tier II |
35 | NC_000072.6 | 6 | 72695703 | - | Tcf7l1 | NNNGAAGCAGAGTTCCTCTG | NGG | 2 | 0.0 | Tier II |
36 | NC_000070.6 | 4 | 138111343 | - | Eif4g3 | NNNGAAGCAGAGTTTCTCTG | NTG | 2 | 0.0 | Tier II |
37 | NC_000071.6 | 5 | 86733097 | - | Tmprss11e | NNNGAAGCAGAGTTTCTCTG | NGC | 2 | 0.0 | Tier II |
38 | NC_000072.6 | 6 | 140443159 | + | Plekha5 | NNNGAAGCAGAGTTTCTCTG | NGT | 2 | 0.0 | Tier II |
39 | NC_000074.6 | 8 | 69965309 | + | Gatad2a | NNNGAAGCAGAGTTTCTCTG | NGT | 2 | 0.0 | Tier II |
40 | NC_000074.6 | 8 | 83080027 | - | Rnf150 | NNNGAAGCAGAGTTTCTCTG | NGT | 2 | 0.0 | Tier II |
41 | NC_000075.6 | 9 | 96621753 | - | Rasa2 | NNNGAAGCAGAGTTTCTCTG | NGT | 2 | 0.0 | Tier II |
42 | NC_000077.6 | 11 | 97992701 | + | Arl5c | NNNGAAGCAGAGTTTCTCTG | NGT | 2 | 0.0 | Tier II |
43 | NC_000068.7 | 2 | 29895763 | - | Odf2 | NNNGAAGCAGGGTTTCTCTG | NGG | 2 | 0.0 | Tier II |
44 | NC_000070.6 | 4 | 129211443 | - | Yars | NNNGAGGCAGAGTTTCTCTG | NGG | 2 | 0.0 | Tier II |
45 | NC_000081.6 | 15 | 15795344 | - | 4930557F08Rik | NNNCAACCAGAGTTTATCTG | NGG | 2 | 0.364 | Tier III |
46 | NC_000072.6 | 6 | 127790828 | + | Gm40402 | NNNGAAGCAGAGGTTATCTG | NGG | 1 | 0.2609 | Tier III |
47 | NC_000084.6 | 18 | 61650056 | - | Carmn | NNNGAAGCAAAGGTTATCTG | NGG | 2 | 0.2435 | Tier III |
48 | NC_000069.6 | 3 | 124012339 | + | Gm40144 | NNNGAAGCAGAGTATAGCTG | NGG | 2 | 0.0728 | Tier III |
49 | NC_000076.6 | 10 | 71527583 | + | Gm34486 | NNNGAAGAAGAGTTTATCTG | NGT | 2 | 0.0105 | Tier III |
50 | NC_000079.6 | 13 | 44029468 | - | Gm33489 | NNNCAAGCAGAGTTTCTCTG | NGG | 2 | 0.0 | Tier III |
51 | NC_000084.6 | 18 | 11008324 | + | 9130001E16Rik | NNNGAAACAGAGTTTCTCTG | NGG | 2 | 0.0 | Tier III |
52 | NC_000072.6 | 6 | 118883098 | + | 4931430N09Rik | NNNGAAGCAGAGCTTCTCTG | NGG | 2 | 0.0 | Tier III |
53 | NC_000077.6 | 11 | 41826100 | - | A230004M16Rik | NNNGAAGCAGAGTGGATCTG | NGG | 2 | 0.0 | Tier III |
54 | NC_000071.6 | 5 | 150841424 | + | Gm36447 | NNNGAAGCAGAGTTCCTCTG | NGG | 2 | 0.0 | Tier III |
55 | NC_000074.6 | 8 | 57492045 | + | 2500002B13Rik | NNNGAAGCAGTGTTTCTCTG | NGG | 2 | 0.0 | Tier III |