Construct: sgRNA BRDN0001145704

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGAAGCAGAGTTTATCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK3 (157)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77946

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 25685216 - GRK3 NNNGAAGCAGAGTTTATCTG NGG 0 1.0 Tier I
2 NC_000007.14 7 30617193 - GARS1 NNNGAAGCGGAGTTTATCTG NAG 2 0.1728 Tier I
3 NC_000023.11 X 136774988 + ARHGEF6 NNNGAAGCAAAGTTTATCTG NGG 1 0.9333 Tier II
4 NC_000018.10 18 27101078 - CHST9 NNNGAACAAGAGTTTATCTG NGG 2 0.4469 Tier II
5 NC_000005.10 5 149632708 + ARHGEF37 NNNGAAGCTGAGTTTATCTT NGG 2 0.42 Tier II
6 NC_000009.12 9 8605123 - PTPRD NNNGAAGAAGAGTTTATTTG NGG 2 0.4179 Tier II
7 NC_000008.11 8 62643665 - NKAIN3 NNNGAAGCAGGGTTTATTTG NGG 2 0.4179 Tier II
8 NC_000011.10 11 124772264 - MSANTD2 NNNGAACCTGAGTTTATCTG NGG 2 0.4125 Tier II
9 NC_000007.14 7 147011617 + CNTNAP2 NNNGAAGCAAAGTTTATCTC NGG 2 0.4 Tier II
10 NC_000007.14 7 34931996 - DPY19L1 NNNGAGGCAGAGTTTAACTG NGG 2 0.381 Tier II
11 NC_000002.12 2 33357353 + LTBP1 NNNGCAGCAGAGATTATCTG NGG 2 0.3462 Tier II
12 NC_000005.10 5 22271065 + CDH12 NNNTAAGCAGAATTTATCTG NGG 2 0.3394 Tier II
13 NC_000017.11 17 73591624 + SDK2 NNNGAAGCAATGTTTATCTG NGG 2 0.2872 Tier II
14 NC_000007.14 7 28160148 + JAZF1 NNNGATGCACAGTTTATCTG NGG 2 0.2857 Tier II
15 NC_000015.10 15 47405712 - SEMA6D NNNGCAGCAGAGTTTATATG NGG 2 0.2692 Tier II
16 NC_000010.11 10 28102786 - MPP7 NNNGAAGCAGATATTATCTG NGG 2 0.2663 Tier II
17 NC_000005.10 5 151370215 + SLC36A1 NNNGAAGCAGATTATATCTG NGG 2 0.2381 Tier II
18 NC_000004.12 4 139517797 + SETD7 NNNGAAGCAGCCTTTATCTG NGG 2 0.2118 Tier II
19 NC_000006.12 6 167939523 - AFDN NNNTCAGCAGAGTTTATCTG NGG 2 0.1818 Tier II
20 NC_000016.10 16 70554252 + SF3B3 NNNGAAGCAGAGTATATCTG NAG 2 0.1605 Tier II
21 NC_000012.12 12 69908523 + MYRFL NNNGGAGCAGAGTTTGTCTG NGG 2 0.1385 Tier II
22 NC_000009.12 9 121599243 + DAB2IP NNNGAAGCAGAGGTTATCTC NGG 2 0.1118 Tier II
23 NC_000008.11 8 40863084 + ZMAT4 NNNGAAGCAGAGGTCATCTG NGG 2 0.0711 Tier II
24 NC_000008.11 8 143876588 - EPPK1 NNNGAAGCAGAGTCTGTCTG NGG 2 0.0549 Tier II
25 NC_000011.10 11 64990300 - BATF2 NNNGAAGCAGAGTTCATGTG NGG 2 0.0364 Tier II
26 NC_000012.12 12 120986241 - HNF1A NNNGGAGCAGAGTTTATCTG NTG 2 0.0281 Tier II
27 NC_000022.11 22 19087001 - DGCR2 NNNGAAGCAGAGTTTATTTG NTG 2 0.025 Tier II
28 NC_000015.10 15 64618686 - ZNF609 NNNGAAGCAGAGTTTGGCTG NGG 2 0.0226 Tier II
29 NC_000011.10 11 45879331 - CRY2 NNNGAAGCAGATTTTATCTG NTG 2 0.015 Tier II
30 NC_000015.10 15 42510965 + SNAP23 NNNGAACCAGAGTTTATCTG NGT 2 0.0111 Tier II
31 NC_000010.11 10 113571671 + HABP2 NNNGAAGCAGAGTTTCACTG NGG 2 0.0 Tier II
32 NC_000002.12 2 166531614 - LOC124906087 NNNAAAGCAGAGTTTATCTA NGG 2 0.8438 Tier III
33 NC_000005.10 5 89282168 + MEF2C-AS1 NNNGATGCAAAGTTTATCTG NGG 2 0.6667 Tier III
34 NC_000020.11 20 38425061 - SNHG17 NNNGAAACAGGGTTTATCTG NGG 2 0.65 Tier III
35 NC_000002.12 2 67115951 + LINC01828 NNNCAAACAGAGTTTATCTG NGG 2 0.5294 Tier III
36 NC_000005.10 5 151370215 + LOC105378234 NNNGAAGCAGATTATATCTG NGG 2 0.2381 Tier III
37 NC_000013.11 13 78098182 + OBI1-AS1 NNNCAAGCAGAGTTTATCTC NGG 2 0.2269 Tier III
38 NC_000005.10 5 88378428 + TMEM161B-DT NNNGAAGCAGAATTTATCTG NTG 2 0.0364 Tier III
39 NC_000001.11 1 219792600 + LOC105372926 NNNAAAGCAGAGTTTATCTG NTG 2 0.0351 Tier III
40 NC_000015.10 15 64618686 - LOC101930091 NNNGAAGCAGAGTTTGGCTG NGG 2 0.0226 Tier III
41 NC_000004.12 4 104607990 + CXXC4-AS1 NNNAAAGCAGAGTTTATCTG NGT 2 0.0145 Tier III
42 NC_000004.12 4 104607990 + LOC124900745 NNNAAAGCAGAGTTTATCTG NGT 2 0.0145 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 61876954 - Palld NNNGAAGCAGAGTTTATTTG NAG 2 0.1667 Tier I
2 NC_000082.6 16 14149077 - Marf1 NNNGAAGCAGAGTTTATCTG NGT 1 0.0161 Tier I
3 NC_000072.6 6 124828160 + Usp5 NNNGAAGCAGAGCTTATCTG NGG 1 0.7895 Tier II
4 NC_000075.6 9 106668660 - Tex264 NNNGAAGCAGAACTTATCTG NGG 2 0.7368 Tier II
5 NC_000076.6 10 18686960 - Arfgef3 NNNGATGCAGAGATTATCTG NGG 2 0.4945 Tier II
6 NC_000069.6 3 27143235 + Ect2 NNNGAACAAGAGTTTATCTG NGG 2 0.4469 Tier II
7 NC_000079.6 13 115049882 - Itga1 NNNGAAGCAGAATTTATCTC NGG 2 0.4 Tier II
8 NC_000074.6 8 82958448 + Rnf150 NNNCATGCAGAGTTTATCTG NGG 2 0.3782 Tier II
9 NC_000077.6 11 75349525 - Smyd4 NNNGAAGGAGAGTTAATCTG NGG 2 0.3722 Tier II
10 NC_000075.6 9 79910791 - Filip1 NNNGCAGAAGAGTTTATCTG NGG 2 0.325 Tier II
11 NC_000081.6 15 35646736 - Vps13b NNNGAAGCAATGTTTATCTG NGG 2 0.2872 Tier II
12 NC_000074.6 8 126322464 + Slc35f3 NNNGAACCAGATTTTATCTG NGG 2 0.2644 Tier II
13 NC_000067.6 1 67147235 - Cps1 NNNAAAGCAGAGTTCATCTG NGG 2 0.2455 Tier II
14 NC_000082.6 16 10742873 - Clec16a NNNGAAGCAGATTATATCTG NGG 2 0.2381 Tier II
15 NC_000067.6 1 88324932 + Trpm8 NNNGAAGAAGAGTTTATCTG NAG 2 0.1685 Tier II
16 NC_000067.6 1 170323473 + Nos1ap NNNGAAGCAGAGTTTATATG NAG 2 0.1396 Tier II
17 NC_000067.6 1 167719634 - Lmx1a NNNGAAGAAGAGTTTGTCTG NGG 2 0.125 Tier II
18 NC_000071.6 5 74699634 - Lnx1 NNNGATGCAGAGTTTATGTG NGG 2 0.0952 Tier II
19 NC_000077.6 11 5761701 + Urgcp NNNGAAGCAGAGTTTAGCTT NGG 2 0.0824 Tier II
20 NC_000071.6 5 115062052 + Sppl3 NNNGAAGCAGAGTTTATCTG NGA 1 0.0694 Tier II
21 NC_000082.6 16 17328543 + Pi4ka NNNGAACCAGAGTTTATCTG NGA 2 0.0477 Tier II
22 NC_000068.7 2 120830317 - Ttbk2 NNNGAAGCAGAGCTGATCTG NGG 2 0.0395 Tier II
23 NC_000073.6 7 57779377 + Gabrb3 NNNGAAGCAGAGTAGATCTG NGG 2 0.031 Tier II
24 NC_000075.6 9 56115735 - Pstpip1 NNNGCAGCAGAGTTTATCTG NTG 2 0.0195 Tier II
25 NC_000068.7 2 92203284 + Phf21a NNNGAAACAGAGTTTATCTG NGT 2 0.0161 Tier II
26 NC_000071.6 5 100865312 + Gpat3 NNNGAAGAAGAGTTTATCTG NGC 2 0.0144 Tier II
27 NC_000079.6 13 59093417 - Ntrk2 NNNGAAGCAGACTTTATCTG NGC 2 0.0118 Tier II
28 NC_000086.7 X 9280347 - Xk NNNGAAGCAGAGTATATCTG NGT 2 0.01 Tier II
29 NC_000085.6 19 22183761 - Trpm3 NNNGAAGCAGAGTTTGTCTG NGT 2 0.0031 Tier II
30 NC_000071.6 5 130415164 + Caln1 NNNGAAGCAGAGTTTATGTG NGT 2 0.0022 Tier II
31 NC_000078.6 12 99748640 - Efcab11 NNNCAAGCAGAGTTTCTCTG NGG 2 0.0 Tier II
32 NC_000084.6 18 11676761 + Rbbp8 NNNGAAACAGAGTTTTTCTG NGG 2 0.0 Tier II
33 NC_000075.6 9 116026720 - Gadl1 NNNGAAGCAGAATTTCTCTG NGG 2 0.0 Tier II
34 NC_000072.6 6 118883098 + Cacna1c NNNGAAGCAGAGCTTCTCTG NGG 2 0.0 Tier II
35 NC_000072.6 6 72695703 - Tcf7l1 NNNGAAGCAGAGTTCCTCTG NGG 2 0.0 Tier II
36 NC_000070.6 4 138111343 - Eif4g3 NNNGAAGCAGAGTTTCTCTG NTG 2 0.0 Tier II
37 NC_000071.6 5 86733097 - Tmprss11e NNNGAAGCAGAGTTTCTCTG NGC 2 0.0 Tier II
38 NC_000072.6 6 140443159 + Plekha5 NNNGAAGCAGAGTTTCTCTG NGT 2 0.0 Tier II
39 NC_000074.6 8 69965309 + Gatad2a NNNGAAGCAGAGTTTCTCTG NGT 2 0.0 Tier II
40 NC_000074.6 8 83080027 - Rnf150 NNNGAAGCAGAGTTTCTCTG NGT 2 0.0 Tier II
41 NC_000075.6 9 96621753 - Rasa2 NNNGAAGCAGAGTTTCTCTG NGT 2 0.0 Tier II
42 NC_000077.6 11 97992701 + Arl5c NNNGAAGCAGAGTTTCTCTG NGT 2 0.0 Tier II
43 NC_000068.7 2 29895763 - Odf2 NNNGAAGCAGGGTTTCTCTG NGG 2 0.0 Tier II
44 NC_000070.6 4 129211443 - Yars NNNGAGGCAGAGTTTCTCTG NGG 2 0.0 Tier II
45 NC_000081.6 15 15795344 - 4930557F08Rik NNNCAACCAGAGTTTATCTG NGG 2 0.364 Tier III
46 NC_000072.6 6 127790828 + Gm40402 NNNGAAGCAGAGGTTATCTG NGG 1 0.2609 Tier III
47 NC_000084.6 18 61650056 - Carmn NNNGAAGCAAAGGTTATCTG NGG 2 0.2435 Tier III
48 NC_000069.6 3 124012339 + Gm40144 NNNGAAGCAGAGTATAGCTG NGG 2 0.0728 Tier III
49 NC_000076.6 10 71527583 + Gm34486 NNNGAAGAAGAGTTTATCTG NGT 2 0.0105 Tier III
50 NC_000079.6 13 44029468 - Gm33489 NNNCAAGCAGAGTTTCTCTG NGG 2 0.0 Tier III
51 NC_000084.6 18 11008324 + 9130001E16Rik NNNGAAACAGAGTTTCTCTG NGG 2 0.0 Tier III
52 NC_000072.6 6 118883098 + 4931430N09Rik NNNGAAGCAGAGCTTCTCTG NGG 2 0.0 Tier III
53 NC_000077.6 11 41826100 - A230004M16Rik NNNGAAGCAGAGTGGATCTG NGG 2 0.0 Tier III
54 NC_000071.6 5 150841424 + Gm36447 NNNGAAGCAGAGTTCCTCTG NGG 2 0.0 Tier III
55 NC_000074.6 8 57492045 + 2500002B13Rik NNNGAAGCAGTGTTTCTCTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)