Construct: sgRNA BRDN0001145707
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGTAGGCAATCACTCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMK1D (57118)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77444
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
12791215 |
- |
CAMK1D |
NNNGTAGGCAATCACTCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
9008270 |
- |
NRIP3-DT |
NNNGTTGGCAATCACTCTGA |
NGG |
2 |
0.4592 |
Tier III |
| 3 |
NC_000023.11 |
X |
102568245 |
+ |
NXF4 |
NNNGTAGGCAATCTCTCCAA |
NGG |
2 |
0.381 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
63322433 |
+ |
Fbxl17 |
NNNGCAGGCAATCACTTCGA |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000083.6 |
17 |
78606528 |
+ |
Vit |
NNNGTTGGCAAGCACTCCGA |
NGG |
2 |
0.3571 |
Tier II |
| 3 |
NC_000067.6 |
1 |
176990977 |
+ |
Sdccag8 |
NNNGTAGGCAATCACTCCTC |
NGG |
2 |
0.1515 |
Tier II |
| 4 |
NC_000082.6 |
16 |
59718234 |
- |
Epha6 |
NNNGTAGGCAAGCACTCCGC |
NGG |
2 |
0.1136 |
Tier II |
| 5 |
NC_000069.6 |
3 |
147145563 |
- |
Gm29771 |
NNNGGAGGCAATCACTCAGA |
NGG |
2 |
0.3446 |
Tier III |
Other clones with same target sequence:
(none)
