Construct: sgRNA BRDN0001145714

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAAAACTTATGTCCATGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBKS (64080)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77040

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27858534 + RBKS NNNAAACTTATGTCCATGGA NGG 0 1.0 Tier I
2 NC_000016.10 16 57755008 + KATNB1 NNNAAATGTATGTCCATGGA NGG 2 0.5958 Tier I
3 NC_000005.10 5 146043935 - SH3RF2 NNNAAACATATGTCCATAGA NGG 2 0.5538 Tier II
4 NC_000018.10 18 12341726 - AFG3L2 NNNAAACCTATGTCCATGCA NGG 2 0.4483 Tier II
5 NC_000010.11 10 70868930 - SGPL1 NNNAAATTTATGTCCAAGGA NGG 2 0.4333 Tier II
6 NC_000003.12 3 174507877 - NAALADL2 NNNAAACTTATATCCATGCA NGG 2 0.4184 Tier II
7 NC_000023.11 X 22119971 + PHEX NNNATACTCATGTCCATGGA NGG 2 0.3357 Tier II
8 NC_000005.10 5 41943456 - FBXO4 NNNAAACTTATGTCCAAGGT NGG 2 0.32 Tier II
9 NC_000006.12 6 30624752 + MRPS18B NNNAAACTGAGGTCCATGGA NGG 2 0.2476 Tier II
10 NC_000006.12 6 70852027 + SMAP1 NNNAAACTTATTTCCATGGT NGG 2 0.2308 Tier II
11 NC_000001.11 1 148814633 - PDE4DIP NNNAAACTCATGTCCATGGA NGA 2 0.0641 Tier II
12 NC_000001.11 1 149315609 + LOC124904395 NNNAAACTCATGTCCATGGA NGA 2 0.0641 Tier II
13 NC_000002.12 2 48655123 - GTF2A1L NNNAAACTTATGTCCATGAA NTG 2 0.0278 Tier II
14 NC_000002.12 2 48655123 - STON1-GTF2A1L NNNAAACTTATGTCCATGAA NTG 2 0.0278 Tier II
15 NC_000013.11 13 94101399 + GPC6 NNNGAACTTATGTCCATGGA NTG 2 0.0244 Tier II
16 NC_000003.12 3 177071488 + TBL1XR1 NNNAAACTTGTGTCCATGGA NTG 2 0.0216 Tier II
17 NC_000003.12 3 151301936 + GPR87 NNNAAACTTATGTCCATGCA NTG 2 0.0175 Tier II
18 NC_000003.12 3 151301936 + MED12L NNNAAACTTATGTCCATGCA NTG 2 0.0175 Tier II
19 NC_000018.10 18 36363330 + FHOD3 NNNAAACTTATGTCCATGGA NAA 2 0.0 Tier II
20 NC_000009.12 9 27227157 - TEK NNNAACCTTATGTGCATGGA NGG 2 0.0 Tier II
21 NC_000011.10 11 90612632 + DISC1FP1 NNNAAACTTTTATCCATGGA NGG 2 0.8235 Tier III
22 NC_000005.10 5 146043935 - LOC107986458 NNNAAACATATGTCCATAGA NGG 2 0.5538 Tier III
23 NC_000018.10 18 12341726 - LOC107985154 NNNAAACCTATGTCCATGCA NGG 2 0.4483 Tier III
24 NC_000001.11 1 100036564 - LOC124904230 NNNAAACTAATTTCCATGGA NGG 2 0.3571 Tier III
25 NC_000001.11 1 185571629 + LINC01350 NNNAACCTTATGTTCATGGA NGG 2 0.3333 Tier III
26 NC_000001.11 1 185571629 + LOC107985239 NNNAACCTTATGTTCATGGA NGG 2 0.3333 Tier III
27 NC_000024.10 Y 20508408 + TTTY10 NNNAAACTTATGTCCGTGGA NGG 1 0.1923 Tier III
28 NC_000018.10 18 30168596 - LOC124904344 NNNAAACTTATGACCATGGC NGG 2 0.1573 Tier III
29 NC_000011.10 11 82297990 + MIR4300HG NNNATACTTCTGTCCATGGA NGG 2 0.1212 Tier III
30 NC_000010.11 10 123423973 + LINC02641 NNNCAACTTATGTCCATGGA NAG 2 0.0915 Tier III
31 NC_000001.11 1 120203194 - LOC100996723 NNNAAACTCATGTCCATGGA NGA 2 0.0641 Tier III
32 NC_000004.12 4 28060736 + LOC105374553 NNNAAACTTATGTCCATGGA NCT 2 0.0 Tier III
33 NC_000006.12 6 74534397 + LOC101928516 NNNAAACTTATGTGTATGGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 31673408 + Rbks NNNAAACTCATGTCCATGGA NGG 1 0.9231 Tier I
2 NC_000081.6 15 21015901 - Cdh12 NNNAGACTTATGTCCATGGA NGG 1 0.72 Tier II
3 NC_000074.6 8 47038974 - Enpp6 NNNTAACTTATATCCATGGA NGG 2 0.5939 Tier II
4 NC_000078.6 12 75776772 + Gm40447 NNNAAACTTATGTCCATGAG NGG 2 0.5462 Tier II
5 NC_000070.6 4 149117966 - Dffa NNNAAACATACGTCCATGGA NGG 2 0.5333 Tier II
6 NC_000084.6 18 35249444 + Ctnna1 NNNAAACTTTTGTCCAAGGA NGG 2 0.4706 Tier II
7 NC_000084.6 18 16729745 + Cdh2 NNNAAACTGATGACCATGGA NGG 2 0.4286 Tier II
8 NC_000070.6 4 14557424 + Slc26a7 NNNAAGCTTGTGTCCATGGA NGG 2 0.3968 Tier II
9 NC_000071.6 5 143713958 - Usp42 NNNAAACTTGTTTCCATGGA NGG 2 0.2137 Tier II
10 NC_000082.6 16 41794840 + Lsamp NNNAAAGTTATTTCCATGGA NGG 2 0.181 Tier II
11 NC_000081.6 15 94656235 - Tmem117 NNNCAACTTCTGTCCATGGA NGG 2 0.1176 Tier II
12 NC_000067.6 1 63753282 - Fastkd2 NNNAAACTTATGTCAATGGA NAG 2 0.0576 Tier II
13 NC_000077.6 11 113963008 + Sdk2 NNNAAACTTGTGTCTATGGA NGG 2 0.037 Tier II
14 NC_000067.6 1 66529261 - Unc80 NNNACACTTATGTCCATGGA NGA 2 0.0347 Tier II
15 NC_000082.6 16 91911084 + Itsn1 NNNAAACTTATGTCCATGGG NTG 2 0.0298 Tier II
16 NC_000070.6 4 117294948 + Rnf220 NNNAAACTGATGTCCATGGA NGC 2 0.0138 Tier II
17 NC_000082.6 16 25327999 + Tprg NNNAAACTTATTTCCCTGGA NGG 2 0.0 Tier II
18 NC_000070.6 4 117294948 + Gm12843 NNNAAACTGATGTCCATGGA NGC 2 0.0138 Tier III
19 NC_000079.6 13 19338405 + Tcrg NNNAAACTCATGTCCTTGGA NGG 2 0.0 Tier III
20 NC_000078.6 12 113820757 + Igh NNNTAACTTATGTCCTTGGA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)