Construct: sgRNA BRDN0001145717
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGGAGCTCCGTGATCTGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTRK1 (4914)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75964
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
156864402 |
+ |
NTRK1 |
NNNGAGCTCCGTGATCTGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
101008910 |
- |
LRRK1 |
NNNGAGCTCCGTGATCTGGC |
NGG |
2 |
0.1607 |
Tier I |
| 3 |
NC_000007.14 |
7 |
93292967 |
+ |
VPS50 |
NNNGAGCTCTGCGATCTGAG |
NGG |
2 |
0.8916 |
Tier II |
| 4 |
NC_000019.10 |
19 |
5079920 |
- |
KDM4B |
NNNGAGCTCCGTGAAATGAG |
NGG |
2 |
0.5789 |
Tier II |
| 5 |
NC_000007.14 |
7 |
45660529 |
+ |
ADCY1 |
NNNGAGCTCTGTGAACTGAG |
NGG |
2 |
0.5449 |
Tier II |
| 6 |
NC_000001.11 |
1 |
53574098 |
+ |
GLIS1 |
NNNGAACTCCGTGATCTGGG |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000016.10 |
16 |
21051646 |
- |
DNAH3 |
NNNGAGCTCCTAGATCTGAG |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000020.11 |
20 |
61882929 |
- |
CDH4 |
NNNGTGCTCCGTGGTCTGAG |
NGG |
2 |
0.1697 |
Tier II |
| 9 |
NC_000015.10 |
15 |
30152530 |
+ |
DNM1P30 |
NNNGAGCTCCTTGGTCTGAG |
NGG |
2 |
0.1795 |
Tier III |
| 10 |
NC_000008.11 |
8 |
79721776 |
+ |
LOC124901965 |
NNNGAGCTCCGTGATGGGAG |
NGG |
2 |
0.0181 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
92424307 |
+ |
Nup54 |
NNNGAGCTGCTTGATCTGAG |
NGG |
2 |
0.2381 |
Tier I |
| 2 |
NC_000070.6 |
4 |
138995933 |
- |
Tmco4 |
NNNGAGCCCTGTGATCTGAG |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000076.6 |
10 |
83561883 |
+ |
Washc4 |
NNNGAGCTCCATGATCTGAA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000076.6 |
10 |
25262703 |
- |
Akap7 |
NNNAAGCCCCGTGATCTGAG |
NGG |
2 |
0.9 |
Tier II |
| 5 |
NC_000068.7 |
2 |
93293020 |
- |
Tspan18 |
NNNGAGCTCAGTGATATGAG |
NGG |
2 |
0.8667 |
Tier II |
| 6 |
NC_000085.6 |
19 |
40167340 |
- |
Cyp2c70 |
NNNGAGCTACATGATCTGAG |
NGG |
2 |
0.8571 |
Tier II |
| 7 |
NC_000069.6 |
3 |
22082206 |
- |
Tbl1xr1 |
NNNGAGCTCCAGGATCTGAG |
NGG |
2 |
0.5 |
Tier II |
| 8 |
NC_000076.6 |
10 |
58505102 |
+ |
Ccdc138 |
NNNGAGCTCAGTGATCTGTG |
NGG |
2 |
0.4667 |
Tier II |
| 9 |
NC_000074.6 |
8 |
104124101 |
+ |
Cdh5 |
NNNAAGCTCCGTGGTCTGAG |
NGG |
2 |
0.42 |
Tier II |
| 10 |
NC_000079.6 |
13 |
68752851 |
+ |
Adcy2 |
NNNCAGCTCCGTGATCAGAG |
NGG |
2 |
0.2824 |
Tier II |
| 11 |
NC_000067.6 |
1 |
129797255 |
+ |
Thsd7b |
NNNGAGCTGCTTGATCTGAG |
NGG |
2 |
0.2381 |
Tier II |
| 12 |
NC_000084.6 |
18 |
63386453 |
- |
Piezo2 |
NNNGAGCCCCGTGAGCTGAG |
NGG |
2 |
0.05 |
Tier II |
| 13 |
NC_000083.6 |
17 |
33744247 |
+ |
Rab11b |
NNNGAGCTCTGTGAGCTGAG |
NGG |
2 |
0.0471 |
Tier II |
| 14 |
NC_000067.6 |
1 |
129797255 |
+ |
Gm15790 |
NNNGAGCTGCTTGATCTGAG |
NGG |
2 |
0.2381 |
Tier III |
Other clones with same target sequence:
(none)
