Construct: sgRNA BRDN0001145720
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGACGTATCATCAAAGTTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACTR2 (10097)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76957
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
65261273 |
+ |
ACTR2 |
NNNCGTATCATCAAAGTTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
5163127 |
- |
AKR1C8 |
NNNCGTGTCATCAAAATTGG |
NGG |
2 |
0.7059 |
Tier II |
| 3 |
NC_000003.12 |
3 |
43310689 |
- |
SNRK |
NNNCGTATCTTCAAAGTTGG |
NAG |
2 |
0.2288 |
Tier II |
| 4 |
NC_000009.12 |
9 |
18236583 |
+ |
ADAMTSL1 |
NNNCTTTTCATCAAAGTTGG |
NGG |
2 |
0.1313 |
Tier II |
| 5 |
NC_000022.11 |
22 |
24687677 |
+ |
ARL5AP4 |
NNNCATATCATCAAAGTTGG |
NGG |
1 |
0.8667 |
Tier III |
| 6 |
NC_000022.11 |
22 |
24687677 |
+ |
LOC100421614 |
NNNCATATCATCAAAGTTGG |
NGG |
1 |
0.8667 |
Tier III |
| 7 |
NC_000003.12 |
3 |
6494988 |
- |
LOC105376944 |
NNNTGGATCATCAAAGTTGG |
NGG |
2 |
0.4571 |
Tier III |
| 8 |
NC_000019.10 |
19 |
20258995 |
+ |
LOC284441 |
NNNGGAATCATCAAAGTTGG |
NGG |
2 |
0.4333 |
Tier III |
| 9 |
NC_000019.10 |
19 |
20258995 |
+ |
LOC105372310 |
NNNGGAATCATCAAAGTTGG |
NGG |
2 |
0.4333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
20464898 |
+ |
Anxa7 |
NNNCATAGCATCAAAGTTGG |
NGG |
2 |
0.6356 |
Tier I |
| 2 |
NC_000077.6 |
11 |
20077308 |
- |
Actr2 |
NNNCGTATCATTAAGGTTGG |
NGG |
2 |
0.35 |
Tier I |
| 3 |
NC_000069.6 |
3 |
59033378 |
+ |
Med12l |
NNNTGTATCATCAGAGTTGG |
NGG |
2 |
0.3733 |
Tier II |
| 4 |
NC_000069.6 |
3 |
122627568 |
- |
Gm43568 |
NNNCGTATGATCACAGTTGG |
NGG |
2 |
0.1327 |
Tier III |
Other clones with same target sequence:
(none)
