Construct: sgRNA BRDN0001145725
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTTTACAATCTCAGGATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK5 (1020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77543
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
151056596 |
+ |
CDK5 |
NNNTTACAATCTCAGGATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
94491538 |
- |
KIAA0825 |
NNNTTACAAACTCAGGATCA |
NGG |
2 |
0.8036 |
Tier II |
| 3 |
NC_000007.14 |
7 |
105685954 |
+ |
ATXN7L1 |
NNNTTAGAATCTCAGGATCA |
NGG |
2 |
0.4412 |
Tier II |
| 4 |
NC_000009.12 |
9 |
129027486 |
+ |
SH3GLB2 |
NNNTTCCAATCTCAGCATCG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000003.12 |
3 |
40895673 |
+ |
LOC105377043 |
NNNTTACACTCTCAGGATGG |
NGG |
2 |
0.0714 |
Tier III |
| 6 |
NC_000008.11 |
8 |
122769570 |
- |
CDK5P1 |
NNNTTACAATCTCAGGATCG |
NGA |
1 |
0.0694 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
103418151 |
+ |
Ddx24 |
NNNTCACAATCTCAGGATGG |
NGG |
2 |
0.125 |
Tier I |
| 2 |
NC_000079.6 |
13 |
38169987 |
- |
Dsp |
NNNTTACAATCTCAGGATCT |
NGG |
1 |
0.7 |
Tier II |
| 3 |
NC_000082.6 |
16 |
4536682 |
- |
Srl |
NNNTTACAATCTCAGGATTT |
NGG |
2 |
0.3231 |
Tier II |
| 4 |
NC_000083.6 |
17 |
63165435 |
+ |
Fbxl17 |
NNNTTACAATCTCAGGATTT |
NGG |
2 |
0.3231 |
Tier II |
| 5 |
NC_000075.6 |
9 |
95833993 |
- |
Pls1 |
NNNTTACAATCTCAGGACTG |
NGG |
2 |
0.1978 |
Tier II |
| 6 |
NC_000085.6 |
19 |
5137948 |
- |
Pacs1 |
NNNTTACAATCTCAGGTTCA |
NGG |
2 |
0.125 |
Tier II |
| 7 |
NC_000080.6 |
14 |
30462771 |
- |
Cacna1d |
NNNTTACAATCTCACGACCG |
NGG |
2 |
0.1169 |
Tier II |
| 8 |
NC_000077.6 |
11 |
21741585 |
+ |
Wdpcp |
NNNTTACAATCTCAGGATCC |
NAG |
2 |
0.1111 |
Tier II |
| 9 |
NC_000080.6 |
14 |
30462771 |
- |
4831407H17Rik |
NNNTTACAATCTCACGACCG |
NGG |
2 |
0.1169 |
Tier III |
Other clones with same target sequence:
(none)
