Construct: sgRNA BRDN0001145727
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCGGGGCACCACGACGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK5 (203054)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77319
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
144391672 |
- |
ADCK5 |
NNNGGGGCACCACGACGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
3186426 |
- |
PRDM16 |
NNNGGGGCACCACCACGCAG |
NGG |
2 |
0.1837 |
Tier I |
| 3 |
NC_000019.10 |
19 |
35299564 |
- |
MAG |
NNNGGGGCACCACGATGTTG |
NGG |
2 |
0.1657 |
Tier I |
| 4 |
NC_000005.10 |
5 |
179307444 |
+ |
ADAMTS2 |
NNNGGGGCACCACCACGCAG |
NGG |
2 |
0.1837 |
Tier II |
| 5 |
NC_000017.11 |
17 |
40053091 |
+ |
MED24 |
NNNGGGGCACCACCACGCAG |
NGG |
2 |
0.1837 |
Tier II |
| 6 |
NC_000011.10 |
11 |
67455177 |
- |
CABP4 |
NNNGGGGCACCAGGACGTGG |
NGG |
2 |
0.0511 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
103787416 |
+ |
Olfr67 |
NNNGGGGCACCACCACATAG |
NGG |
2 |
0.4 |
Tier I |
| 2 |
NC_000068.7 |
2 |
103299941 |
- |
Ehf |
NNNGGGGCACCACTACGTTG |
NGG |
2 |
0.1436 |
Tier II |
| 3 |
NC_000080.6 |
14 |
31317770 |
- |
Dnah1 |
NNNGGGGTACCAGGACGTAG |
NGG |
2 |
0.1193 |
Tier II |
| 4 |
NC_000077.6 |
11 |
98328477 |
- |
Neurod2 |
NNNGGGGCAGCAGGACGTAG |
NGG |
2 |
0.053 |
Tier II |
| 5 |
NC_000080.6 |
14 |
31317770 |
- |
Gm35823 |
NNNGGGGTACCAGGACGTAG |
NGG |
2 |
0.1193 |
Tier III |
Other clones with same target sequence:
(none)
