Construct: sgRNA BRDN0001145731

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGCAGATGATCCAAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA6 (27330)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75533

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 84135159 + RPS6KA6 NNNCAGATGATCCAAAGCAA NGG 0 1.0 Tier I
2 NC_000006.12 6 142773297 - HIVEP2 NNNCAGCTGATCCCAAGCAA NGG 2 0.0938 Tier I
3 NC_000005.10 5 31316219 + CDH6 NNNCAGATAATCCAAAGCAA NGT 2 0.0104 Tier I
4 NC_000001.11 1 170728894 + PRRX1 NNNCAAGTGATCCAAAGCAA NGG 2 0.7059 Tier II
5 NC_000009.12 9 111412385 + ECPAS NNNCAGATGATCTAAAACAA NGG 2 0.6533 Tier II
6 NC_000007.14 7 73574715 - TBL2 NNNCAAATGATCCAAAGCAT NGG 2 0.6 Tier II
7 NC_000001.11 1 196319938 + KCNT2 NNNTAGATGATCTAAAGCAA NGG 2 0.56 Tier II
8 NC_000002.12 2 62707119 - EHBP1 NNNCAGAGGGTCCAAAGCAA NGG 2 0.4074 Tier II
9 NC_000004.12 4 120699777 + PRDM5 NNNCAGATGATACAAAGAAA NGG 2 0.3846 Tier II
10 NC_000003.12 3 155610427 + PLCH1 NNNCAGATTATCTAAAGCAA NGG 2 0.3733 Tier II
11 NC_000023.11 X 32340654 + DMD NNNCAGATAATTCAAAGCAA NGG 2 0.3462 Tier II
12 NC_000014.9 14 67427025 + GPHN NNNTAGATGAGCCAAAGCAA NGG 2 0.32 Tier II
13 NC_000005.10 5 149248352 - ABLIM3 NNNCAGATGCCCCAAAGCAA NGG 2 0.2222 Tier II
14 NC_000012.12 12 70256310 - CNOT2 NNNCAGATGTTCCAAATCAA NGG 2 0.2206 Tier II
15 NC_000011.10 11 74359218 - PGM2L1 NNNCAGATGATCAGAAGCAA NGG 2 0.1795 Tier II
16 NC_000017.11 17 51291994 - UTP18 NNNCAGATGATCCAAAGCAT NAG 2 0.1556 Tier II
17 NC_000012.12 12 2612682 - CACNA1C NNNCAGATGATCCAAAGAAC NGG 2 0.1224 Tier II
18 NC_000002.12 2 158486801 - PKP4 NNNCAGATCATCCCAAGCAA NGG 2 0.1154 Tier II
19 NC_000008.11 8 69480076 - SULF1 NNNCAGATCATCCATAGCAA NGG 2 0.1077 Tier II
20 NC_000012.12 12 124988767 - DHX37 NNNCAGATGATCCAAAGGAG NGG 2 0.102 Tier II
21 NC_000015.10 15 29512016 - ENTREP2 NNNCAGATGAGCCAAAGCAC NGG 2 0.0909 Tier II
22 NC_000002.12 2 72448701 - EXOC6B NNNCAGATGCTCCAAACCAA NGG 2 0.0784 Tier II
23 NC_000006.12 6 148447556 - SASH1 NNNCAGATGATCCAAAGCAG NGA 2 0.0531 Tier II
24 NC_000001.11 1 18338323 - IGSF21 NNNCAGATGAACCAAAGCAA NGA 2 0.0521 Tier II
25 NC_000009.12 9 93245964 + WNK2 NNNCAGATGATCCACAGGAA NGG 2 0.0364 Tier II
26 NC_000004.12 4 48386976 + SLAIN2 NNNCAGATGATCCAAAACAA NGT 2 0.0151 Tier II
27 NC_000023.11 X 25862292 + LOC107985707 NNNCAGATGAACCAAAGCTA NGG 2 0.4038 Tier III
28 NC_000001.11 1 101073452 - DPH5-DT NNNCAGATGATCAAAAGCAG NGG 2 0.2941 Tier III
29 NC_000018.10 18 47491754 + MIR4527HG NNNCAGATGATCCAAAGCAG NAG 2 0.1983 Tier III
30 NC_000011.10 11 74359218 - LOC112268078 NNNCAGATGATCAGAAGCAA NGG 2 0.1795 Tier III
31 NC_000011.10 11 82233045 - MIR4300HG NNNCAGATGATCCATAGCAA NGC 2 0.0044 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 50800179 - Alg9 NNNCAGAAGAGCCAAAGCAA NGG 2 0.32 Tier I
2 NC_000075.6 9 107996783 - Cdhr4 NNNCAGACGATCCACAGCAA NGG 2 0.2727 Tier I
3 NC_000086.7 X 111421725 + Rps6ka6 NNNTAGATGATCCAAAGCCA NGG 2 0.1655 Tier I
4 NC_000081.6 15 89324094 - Adm2 NNNCAGATGAACCATAGCAA NGG 2 0.15 Tier I
5 NC_000076.6 10 93051303 + Cfap54 NNNCAGAAGATCCAAAGCAA NGG 1 0.8 Tier II
6 NC_000082.6 16 45872949 - Phldb2 NNNTGGATGATCCAAAGCAA NGG 2 0.576 Tier II
7 NC_000078.6 12 117000106 - Ptprn2 NNNCAGATAACCCAAAGCAA NGG 2 0.4286 Tier II
8 NC_000075.6 9 35205186 - Foxred1 NNNCAGATGATCCTGAGCAA NGG 2 0.3467 Tier II
9 NC_000072.6 6 67015008 - Gng12 NNNCAGATGATGCAAAGCAG NGG 2 0.3399 Tier II
10 NC_000084.6 18 77705190 + 8030462N17Rik NNNCAGATGATTCAAAGCAT NGG 2 0.3231 Tier II
11 NC_000067.6 1 69876145 - Spag16 NNNGAGATAATCCAAAGCAA NGG 2 0.3214 Tier II
12 NC_000077.6 11 33476625 + Ranbp17 NNNCTGATGTTCCAAAGCAA NGG 2 0.3209 Tier II
13 NC_000083.6 17 70044195 - Dlgap1 NNNCAGGTGATCAAAAGCAA NGG 2 0.2715 Tier II
14 NC_000077.6 11 109993109 - Abca8b NNNGAGATTATCCAAAGCAA NGG 2 0.2667 Tier II
15 NC_000077.6 11 66263008 - Shisa6 NNNCAGATGATCCAAAGTAA NAG 2 0.1667 Tier II
16 NC_000068.7 2 162963619 + L3mbtl1 NNNCACATGATCCCAAGCAA NGG 2 0.1461 Tier II
17 NC_000079.6 13 8343296 - Adarb2 NNNCAGATGAACCAAGGCAA NGG 2 0.1442 Tier II
18 NC_000070.6 4 97736785 - Nfia NNNCAGAGGATCCAAGGCAA NGG 2 0.141 Tier II
19 NC_000081.6 15 72507883 + Kcnk9 NNNCAGATCATCCAAACCAA NGG 2 0.1267 Tier II
20 NC_000080.6 14 123285874 + Nalcn NNNCAGATGATGCAAAGCAA NAG 2 0.1152 Tier II
21 NC_000075.6 9 103488681 - Tmem108 NNNCAGATGGTCCAAAGGAA NGG 2 0.0741 Tier II
22 NC_000076.6 10 74251359 + Pcdh15 NNNCAAATGATCCAAAGCAA NGA 2 0.0694 Tier II
23 NC_000071.6 5 37348946 - Evc2 NNNCAGATGATGGAAAGCAA NGG 2 0.0606 Tier II
24 NC_000075.6 9 69419965 - Ice2 NNNCAGATGATGCAAAGCAA NGA 2 0.0309 Tier II
25 NC_000080.6 14 59912280 + Atp8a2 NNNCAGCTGATCCAAAGCAA NGA 2 0.0304 Tier II
26 NC_000074.6 8 69157916 + Lzts1 NNNCAGTTGATCCAAAGCAA NGA 2 0.0304 Tier II
27 NC_000068.7 2 35928485 - Ttll11 NNNCAGATGATCCAAAGGCA NGG 2 0.0276 Tier II
28 NC_000067.6 1 97948234 + Pam NNNCAGATGATCCAAAGCAA NGT 1 0.0161 Tier II
29 NC_000080.6 14 10529315 + Fhit NNNCAGATCATCCAATGCAA NGG 2 0.0 Tier II
30 NC_000076.6 10 45631361 - Hace1 NNNCAGATGATCCAAAGCAA NAA 2 0.0 Tier II
31 NC_000070.6 4 125268457 + Gm32611 NNNCAGGTGACCCAAAGCAA NGG 2 0.4706 Tier III
32 NC_000073.6 7 131824934 + Gm34645 NNNGAGATGATTCAAAGCAA NGG 2 0.2692 Tier III
33 NC_000077.6 11 66263008 - Gm12296 NNNCAGATGATCCAAAGTAA NAG 2 0.1667 Tier III
34 NC_000075.6 9 103488681 - Gm16252 NNNCAGATGGTCCAAAGGAA NGG 2 0.0741 Tier III
35 NC_000074.6 8 69157916 + Gm39187 NNNCAGTTGATCCAAAGCAA NGA 2 0.0304 Tier III
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Other clones with same target sequence:

(none)