Construct: sgRNA BRDN0001145734

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTGATGATAAATGACTGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75543

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 140800486 + PTK2 NNNGATGATAAATGACTGCG NGG 0 1.0 Tier I
2 NC_000011.10 11 47573447 + KBTBD4 NNNGATAATAAATGACTGCG NTG 2 0.039 Tier I
3 NC_000006.12 6 125041265 - RNF217 NNNGATGATAGATGACTGAG NGG 2 0.2786 Tier II
4 NC_000003.12 3 170546298 + SLC7A14 NNNGTTGATAAATGACTGAG NGG 2 0.1558 Tier II
5 NC_000023.11 X 107079812 - RBM41 NNNGATGATAAATGACTGCC NAG 2 0.1111 Tier II
6 NC_000004.12 4 56429778 - PPAT NNNGATGATTAATGACTGGG NGG 2 0.1103 Tier II
7 NC_000004.12 4 56429778 - PAICS NNNGATGATTAATGACTGGG NGG 2 0.1103 Tier II
8 NC_000018.10 18 37534245 - CELF4 NNNGATGATTAATGACGGCG NGG 2 0.1038 Tier II
9 NC_000011.10 11 47573447 + PTPMT1 NNNGATAATAAATGACTGCG NTG 2 0.039 Tier II
10 NC_000011.10 11 113199871 + NCAM1 NNNGATGATAAATGTGTGCG NGG 2 0.0308 Tier II
11 NC_000001.11 1 234758449 - LINC02961 NNNGATGATAAACGACCGCG NGG 2 0.5573 Tier III
12 NC_000001.11 1 234758449 - LOC107985364 NNNGATGATAAACGACCGCG NGG 2 0.5573 Tier III
13 NC_000010.11 10 64421876 - LOC124902439 NNNGATGAAACATGACTGCG NGG 2 0.3714 Tier III
14 NC_000003.12 3 170546298 + SLC7A14-AS1 NNNGTTGATAAATGACTGAG NGG 2 0.1558 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 33885112 - Mvb12b NNNGATGACAAATGACTGCA NGG 2 0.8654 Tier II
2 NC_000075.6 9 118555508 - Golga4 NNNGATGATAAAAGACTGCA NGG 2 0.649 Tier II
3 NC_000068.7 2 164153610 + Stk4 NNNGATGATAAATGGCTGCT NGG 2 0.455 Tier II
4 NC_000070.6 4 9518364 + Asph NNNAATGATAAATGACTGCC NGG 2 0.3857 Tier II
5 NC_000070.6 4 116639436 - Akr1a1 NNNGATGATAAATGACATCG NGG 2 0.3556 Tier II
6 NC_000082.6 16 29335728 + Atp13a5 NNNGAGGATAAATGACTGAG NGG 2 0.2449 Tier II
7 NC_000073.6 7 135058601 - Dock1 NNNCATGATAAATGACTGTG NGG 2 0.2443 Tier II
8 NC_000071.6 5 102224459 - Arhgap24 NNNCATGATAAATGACTGAG NGG 2 0.2269 Tier II
9 NC_000074.6 8 9609006 + Fam155a NNNGATGATAAATGACTGAC NGG 2 0.1837 Tier II
10 NC_000073.6 7 12978833 - Zfp446 NNNGAAGATAAATGACTGGG NGG 2 0.1083 Tier II
11 NC_000083.6 17 57325354 - Vav1 NNNGATGATAAATGAGTGAG NGG 2 0.0659 Tier II
12 NC_000070.6 4 40314264 - 4930509K18Rik NNNGATGATAAATGCCGGCG NGG 2 0.0321 Tier III
Download CSV

Other clones with same target sequence:

(none)

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