Construct: sgRNA BRDN0001145734
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGATGATAAATGACTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK2 (5747)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75543
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
140800486 |
+ |
PTK2 |
NNNGATGATAAATGACTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
47573447 |
+ |
KBTBD4 |
NNNGATAATAAATGACTGCG |
NTG |
2 |
0.039 |
Tier I |
| 3 |
NC_000006.12 |
6 |
125041265 |
- |
RNF217 |
NNNGATGATAGATGACTGAG |
NGG |
2 |
0.2786 |
Tier II |
| 4 |
NC_000003.12 |
3 |
170546298 |
+ |
SLC7A14 |
NNNGTTGATAAATGACTGAG |
NGG |
2 |
0.1558 |
Tier II |
| 5 |
NC_000023.11 |
X |
107079812 |
- |
RBM41 |
NNNGATGATAAATGACTGCC |
NAG |
2 |
0.1111 |
Tier II |
| 6 |
NC_000004.12 |
4 |
56429778 |
- |
PPAT |
NNNGATGATTAATGACTGGG |
NGG |
2 |
0.1103 |
Tier II |
| 7 |
NC_000004.12 |
4 |
56429778 |
- |
PAICS |
NNNGATGATTAATGACTGGG |
NGG |
2 |
0.1103 |
Tier II |
| 8 |
NC_000018.10 |
18 |
37534245 |
- |
CELF4 |
NNNGATGATTAATGACGGCG |
NGG |
2 |
0.1038 |
Tier II |
| 9 |
NC_000011.10 |
11 |
47573447 |
+ |
PTPMT1 |
NNNGATAATAAATGACTGCG |
NTG |
2 |
0.039 |
Tier II |
| 10 |
NC_000011.10 |
11 |
113199871 |
+ |
NCAM1 |
NNNGATGATAAATGTGTGCG |
NGG |
2 |
0.0308 |
Tier II |
| 11 |
NC_000001.11 |
1 |
234758449 |
- |
LINC02961 |
NNNGATGATAAACGACCGCG |
NGG |
2 |
0.5573 |
Tier III |
| 12 |
NC_000001.11 |
1 |
234758449 |
- |
LOC107985364 |
NNNGATGATAAACGACCGCG |
NGG |
2 |
0.5573 |
Tier III |
| 13 |
NC_000010.11 |
10 |
64421876 |
- |
LOC124902439 |
NNNGATGAAACATGACTGCG |
NGG |
2 |
0.3714 |
Tier III |
| 14 |
NC_000003.12 |
3 |
170546298 |
+ |
SLC7A14-AS1 |
NNNGTTGATAAATGACTGAG |
NGG |
2 |
0.1558 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
33885112 |
- |
Mvb12b |
NNNGATGACAAATGACTGCA |
NGG |
2 |
0.8654 |
Tier II |
| 2 |
NC_000075.6 |
9 |
118555508 |
- |
Golga4 |
NNNGATGATAAAAGACTGCA |
NGG |
2 |
0.649 |
Tier II |
| 3 |
NC_000068.7 |
2 |
164153610 |
+ |
Stk4 |
NNNGATGATAAATGGCTGCT |
NGG |
2 |
0.455 |
Tier II |
| 4 |
NC_000070.6 |
4 |
9518364 |
+ |
Asph |
NNNAATGATAAATGACTGCC |
NGG |
2 |
0.3857 |
Tier II |
| 5 |
NC_000070.6 |
4 |
116639436 |
- |
Akr1a1 |
NNNGATGATAAATGACATCG |
NGG |
2 |
0.3556 |
Tier II |
| 6 |
NC_000082.6 |
16 |
29335728 |
+ |
Atp13a5 |
NNNGAGGATAAATGACTGAG |
NGG |
2 |
0.2449 |
Tier II |
| 7 |
NC_000073.6 |
7 |
135058601 |
- |
Dock1 |
NNNCATGATAAATGACTGTG |
NGG |
2 |
0.2443 |
Tier II |
| 8 |
NC_000071.6 |
5 |
102224459 |
- |
Arhgap24 |
NNNCATGATAAATGACTGAG |
NGG |
2 |
0.2269 |
Tier II |
| 9 |
NC_000074.6 |
8 |
9609006 |
+ |
Fam155a |
NNNGATGATAAATGACTGAC |
NGG |
2 |
0.1837 |
Tier II |
| 10 |
NC_000073.6 |
7 |
12978833 |
- |
Zfp446 |
NNNGAAGATAAATGACTGGG |
NGG |
2 |
0.1083 |
Tier II |
| 11 |
NC_000083.6 |
17 |
57325354 |
- |
Vav1 |
NNNGATGATAAATGAGTGAG |
NGG |
2 |
0.0659 |
Tier II |
| 12 |
NC_000070.6 |
4 |
40314264 |
- |
4930509K18Rik |
NNNGATGATAAATGCCGGCG |
NGG |
2 |
0.0321 |
Tier III |
Other clones with same target sequence:
(none)
