Construct: sgRNA BRDN0001145740
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAAGAAGACAACGACACGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KIT (3815)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77560
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
54698341 |
+ |
KIT |
NNNAGAAGACAACGACACGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
62892393 |
- |
PHLPP1 |
NNNAGAAGACAACGACACTC |
NGG |
1 |
0.6667 |
Tier II |
| 3 |
NC_000019.10 |
19 |
46715897 |
+ |
PRKD2 |
NNNAGAAAACAACGACATGC |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000012.12 |
12 |
12339076 |
+ |
MANSC1 |
NNNAGAAGACAACCACACAC |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000001.11 |
1 |
224535243 |
+ |
CNIH3 |
NNNAGAAGACAGAGACACGC |
NGG |
2 |
0.2778 |
Tier II |
| 6 |
NC_000010.11 |
10 |
70685903 |
- |
ADAMTS14 |
NNNAGAAGACAATGACACGC |
NGA |
2 |
0.0486 |
Tier II |
| 7 |
NC_000012.12 |
12 |
12339076 |
+ |
RPL23AP66 |
NNNAGAAGACAACCACACAC |
NGG |
2 |
0.3061 |
Tier III |
| 8 |
NC_000014.9 |
14 |
22420415 |
+ |
TRA |
NNNTGAAGACAAAGACACGC |
NGG |
2 |
0.2448 |
Tier III |
| 9 |
NC_000014.9 |
14 |
22420415 |
+ |
TRD-AS1 |
NNNTGAAGACAAAGACACGC |
NGG |
2 |
0.2448 |
Tier III |
| 10 |
NC_000008.11 |
8 |
56652624 |
+ |
LOC105375851 |
NNNAGAAGACAGGGACACGC |
NGG |
2 |
0.0985 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
75609327 |
+ |
Kit |
NNNAGAAGACAGCGACGCGC |
NGG |
2 |
0.1275 |
Tier I |
| 2 |
NC_000073.6 |
7 |
99069609 |
+ |
Uvrag |
NNNAGAAGACAATGACACGC |
NGG |
1 |
0.7 |
Tier II |
| 3 |
NC_000068.7 |
2 |
181007289 |
- |
Col20a1 |
NNNAGAAGACAACCACATGC |
NGG |
2 |
0.2755 |
Tier II |
| 4 |
NC_000086.7 |
X |
84476858 |
+ |
Dmd |
NNNAGAAGACATTGACACGC |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000068.7 |
2 |
34596472 |
- |
Mapkap1 |
NNNAGAAGACAAAGACAAGC |
NGG |
2 |
0.2071 |
Tier II |
Other clones with same target sequence:
(none)
