Construct: sgRNA BRDN0001145745

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGTGGTGCATATGCAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKG2 (5593)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76920

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 81204782 - PRKG2 NNNGTGGTGCATATGCAGGG NGG 0 1.0 Tier I
2 NC_000001.11 1 19087836 - UBR4 NNNGTGCTGCATCTGCAGGG NGG 2 0.1447 Tier I
3 NC_000021.9 21 44391498 - TRPM2 NNNGTGGTGCATCTGCAGGC NGG 2 0.0902 Tier I
4 NC_000011.10 11 4640621 + OR51D1 NNNGTGGTGCATAGGCTGGG NGG 2 0.0 Tier I
5 NC_000018.10 18 36321792 + FHOD3 NNNGTGGTGAATATGCAGGG NGG 1 0.8667 Tier II
6 NC_000005.10 5 33583357 - ADAMTS12 NNNGTGGTGCATATGCATGG NGG 1 0.6667 Tier II
7 NC_000003.12 3 123154925 - PDIA5 NNNGTGATGCATGTGCAGGG NGG 2 0.6522 Tier II
8 NC_000004.12 4 36033038 - ARAP2 NNNGTGGTGCATATACAGTG NGG 2 0.6275 Tier II
9 NC_000008.11 8 1217322 - DLGAP2 NNNGTGGTGCATATGCATGA NGG 2 0.625 Tier II
10 NC_000018.10 18 26017904 - SS18 NNNGTGGTGAATATGCAAGG NGG 2 0.6 Tier II
11 NC_000016.10 16 11756616 - ZC3H7A NNNGTCGTGCAAATGCAGGG NGG 2 0.5455 Tier II
12 NC_000012.12 12 86798439 - MGAT4C NNNGTTGTGCATGTGCAGGG NGG 2 0.4348 Tier II
13 NC_000005.10 5 60332553 - PDE4D NNNGTGGTACATATGCAGTG NGG 2 0.4286 Tier II
14 NC_000003.12 3 134630702 + CEP63 NNNGTGGTGCACATGCAGGG NAG 2 0.2456 Tier II
15 NC_000003.12 3 134630702 + KY NNNGTGGTGCACATGCAGGG NAG 2 0.2456 Tier II
16 NC_000017.11 17 45809045 + CRHR1 NNNGTGGTGCAAATCCAGGG NGG 2 0.2182 Tier II
17 NC_000017.11 17 45809045 + LINC02210-CRHR1 NNNGTGGTGCAAATCCAGGG NGG 2 0.2182 Tier II
18 NC_000011.10 11 67996854 - UNC93B1 NNNGTGGTGCATCTGAAGGG NGG 2 0.2105 Tier II
19 NC_000017.11 17 82567044 + FOXK2 NNNGTGGTGCATATTCATGG NGG 2 0.0952 Tier II
20 NC_000006.12 6 32336485 - TSBP1 NNNGTGGTGCATATGCAGGT NTG 2 0.0273 Tier II
21 NC_000004.12 4 185689482 + SORBS2 NNNGTGGTGCATATGCAGGG NTA 2 0.0 Tier II
22 NC_000008.11 8 60097049 - LOC105375861 NNNGTGGTGAATATGCAGAG NGG 2 0.619 Tier III
23 NC_000023.11 X 119303031 - LINC03098 NNNTTGGAGCATATGCAGGG NGG 2 0.2909 Tier III
24 NC_000011.10 11 130390735 + ZBTB44-DT NNNGTGGTGGATATGCAGGC NGG 2 0.1667 Tier III
25 NC_000001.11 1 47408642 - LINC01389 NNNGTGGTGGTTATGCAGGG NGG 2 0.1197 Tier III
26 NC_000006.12 6 32336485 - TSBP1-AS1 NNNGTGGTGCATATGCAGGT NTG 2 0.0273 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 139477222 + Ubr4 NNNGTGCTGCATCTGCAGGG NGG 2 0.1447 Tier I
2 NC_000076.6 10 122683546 - Ppm1h NNNGTGGGGAATATGCAGGG NGG 2 0.6356 Tier II
3 NC_000071.6 5 125512986 + Aacs NNNGTTGTGCACATGCAGGG NGG 2 0.6316 Tier II
4 NC_000067.6 1 123418613 + Dpp10 NNNGTGGTGCATGTGCAGGA NGG 2 0.6114 Tier II
5 NC_000067.6 1 167063910 + Fam78b NNNGTGGTGCATGTGCAGGA NGG 2 0.6114 Tier II
6 NC_000068.7 2 59052326 + Ccdc148 NNNGTGTTGCACATGCAGGG NGG 2 0.5414 Tier II
7 NC_000073.6 7 98052840 - Myo7a NNNGTGGAGCGTATGCAGGG NGG 2 0.52 Tier II
8 NC_000069.6 3 136137640 - Bank1 NNNGTGGTGCATATACACGG NGG 2 0.4482 Tier II
9 NC_000073.6 7 91418016 + Dlg2 NNNGTGGTGCATATGAAGGC NGG 2 0.4286 Tier II
10 NC_000067.6 1 189216227 + Kcnk2 NNNGTGGTGCACATGCAGGC NGG 2 0.406 Tier II
11 NC_000072.6 6 119213277 + Dcp1b NNNGTGGTGCATATGCACTG NGG 2 0.3175 Tier II
12 NC_000071.6 5 75579861 + Kit NNNGGGGTGCATATGCACGG NGG 2 0.3048 Tier II
13 NC_000083.6 17 70775301 + Dlgap1 NNNGTGGTGCATATGCATGC NGG 2 0.2857 Tier II
14 NC_000075.6 9 37121811 + Pknox2 NNNGTGGTGGAGATGCAGGG NGG 2 0.1944 Tier II
15 NC_000071.6 5 81255014 - Adgrl3 NNNGTGGTGCATATGCAGGC NAG 2 0.1111 Tier II
16 NC_000074.6 8 78512470 + Slc10a7 NNNGTGGTGCATATGGAGGT NGG 2 0.1077 Tier II
17 NC_000073.6 7 91915868 - Dlg2 NNNGGGGTGCATATTCAGGG NGG 2 0.0914 Tier II
18 NC_000085.6 19 36039235 - Htr7 NNNGTGGTGCAGATGGAGGG NGG 2 0.0769 Tier II
19 NC_000078.6 12 80239654 - Actn1 NNNGTGGGGCATATGCAGGG NGA 2 0.0509 Tier II
20 NC_000077.6 11 58185820 - Gm12250 NNNGTGGTGCACATGCAGGG NTG 2 0.0369 Tier II
21 NC_000075.6 9 54945223 - Hykk NNNATGGTGCATATGCAGGG NTG 2 0.0351 Tier II
22 NC_000067.6 1 77463568 - Epha4 NNNGTGGTGCATATGCAGGT NTG 2 0.0273 Tier II
23 NC_000067.6 1 172202106 - Pea15a NNNGTGGTGCATGGGCAGGG NGG 2 0.0 Tier II
24 NC_000080.6 14 29541759 - Cacna2d3 NNNGTGGTGCATGGGCAGGG NGG 2 0.0 Tier II
25 NC_000083.6 17 70775301 + Gm41609 NNNGTGGTGCATATGCATGC NGG 2 0.2857 Tier III
26 NC_000084.6 18 61796230 + Gm35919 NNNTGGGTGCATATGCAGGG NGG 2 0.2327 Tier III
27 NC_000072.6 6 86128440 - Gm19596 NNNGTGGTTCATATGCAGGG NTG 2 0.0208 Tier III
28 NC_000078.6 12 104404072 - Gm40561 NNNGAGGTGCATAGGCAGGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)