Construct: sgRNA BRDN0001145753
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACGGTCATGATGTACGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKB (1152)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76590
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
103522171 |
+ |
CKB |
NNNCGGTCATGATGTACGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
103510913 |
- |
RELN |
NNNCAGTCATGATGGACGGG |
NGG |
2 |
0.0433 |
Tier I |
| 3 |
NC_000021.9 |
21 |
8199201 |
+ |
LOC124904993 |
NNNCGGTGATGATGAACGGG |
NGG |
2 |
0.3722 |
Tier III |
| 4 |
NT_167214.1 |
|
98702 |
+ |
LOC100507412 |
NNNCGGTGATGATGAACGGG |
NGG |
2 |
0.3722 |
Tier III |
| 5 |
NT_187388.1 |
22 |
115438 |
+ |
LOC107987392 |
NNNCGGTGATGATGAACGGG |
NGG |
2 |
0.3722 |
Tier III |
| 6 |
NC_000007.14 |
7 |
103510913 |
- |
SLC26A5-AS1 |
NNNCAGTCATGATGGACGGG |
NGG |
2 |
0.0433 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
111671573 |
+ |
Ckb |
NNNCAGTCATGATGTACGGG |
NGG |
1 |
0.8667 |
Tier I |
| 2 |
NC_000082.6 |
16 |
38483443 |
- |
Cd80 |
NNNCTGTCATGATGTACGGG |
NGG |
1 |
0.3 |
Tier II |
| 3 |
NC_000083.6 |
17 |
53941908 |
+ |
Gm6949 |
NNNCAGTCATGATGTACTGG |
NGG |
2 |
0.5778 |
Tier III |
| 4 |
NC_000083.6 |
17 |
53941908 |
+ |
Ckb-ps1 |
NNNCAGTCATGATGTACTGG |
NGG |
2 |
0.5778 |
Tier III |
| 5 |
NC_000082.6 |
16 |
38483443 |
- |
Ck-ps3 |
NNNCTGTCATGATGTACGGG |
NGG |
1 |
0.3 |
Tier III |
| 6 |
NC_000070.6 |
4 |
122008393 |
- |
Gm12892 |
NNNCTGTCATGATATACGGG |
NGG |
2 |
0.225 |
Tier III |
Other clones with same target sequence:
(none)
