Construct: sgRNA BRDN0001145757
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAAGATGATGAAACACCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C2A (5286)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76416
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
17145924 |
- |
PIK3C2A |
NNNAGATGATGAAACACCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
70376112 |
+ |
KIF4A |
NNNAGATGATGAAAAACCAG |
NGG |
2 |
0.0952 |
Tier I |
| 3 |
NC_000011.10 |
11 |
7239323 |
- |
SYT9 |
NNNGGATGATGAAACACCCA |
NGG |
2 |
0.5859 |
Tier II |
| 4 |
NC_000016.10 |
16 |
83536955 |
- |
CDH13 |
NNNAGAAGATGAAACACCTG |
NGG |
2 |
0.4038 |
Tier II |
| 5 |
NC_000007.14 |
7 |
99284644 |
+ |
MYH16 |
NNNGGATGATGAAACACCTG |
NGG |
2 |
0.2885 |
Tier II |
| 6 |
NC_000010.11 |
10 |
126512061 |
- |
C10orf90 |
NNNAGATGATGAAACACTCC |
NGG |
2 |
0.2755 |
Tier II |
| 7 |
NC_000010.11 |
10 |
95610707 |
+ |
ALDH18A1 |
NNNAGAGGATGAAACACCTG |
NGG |
2 |
0.2715 |
Tier II |
| 8 |
NC_000001.11 |
1 |
239427306 |
+ |
CHRM3 |
NNNAGATGATGAAACACACC |
NGG |
2 |
0.2308 |
Tier II |
| 9 |
NC_000012.12 |
12 |
4281588 |
+ |
CCND2 |
NNNAGCTGATGAAACACCTG |
NGG |
2 |
0.2098 |
Tier II |
| 10 |
NC_000007.14 |
7 |
99284644 |
+ |
LOC105375421 |
NNNGGATGATGAAACACCTG |
NGG |
2 |
0.2885 |
Tier III |
| 11 |
NC_000015.10 |
15 |
61577984 |
- |
LOC107984782 |
NNNAGATGAGGAAACACCTG |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
141765688 |
+ |
Gm5724 |
NNNAGATTATGAAACACCCA |
NGG |
2 |
0.5859 |
Tier I |
| 2 |
NC_000073.6 |
7 |
116388103 |
- |
Pik3c2a |
NNNAGATGATGAAGCACCTG |
NGG |
2 |
0.2154 |
Tier I |
| 3 |
NC_000079.6 |
13 |
59687674 |
- |
4921517D22Rik |
NNNAAATGATGAAACACACG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000070.6 |
4 |
24540178 |
- |
Mms22l |
NNNATATGATGAAACACCAG |
NGG |
2 |
0.1286 |
Tier II |
| 5 |
NC_000077.6 |
11 |
58924068 |
+ |
Btnl10 |
NNNATATGATGAAACACCAG |
NGG |
2 |
0.1286 |
Tier II |
| 6 |
NC_000072.6 |
6 |
34034794 |
+ |
Lrguk |
NNNAGCTGATGAAACTCCCG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
