Construct: sgRNA BRDN0001145761

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTGCTGCCATCAAAGATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDK2 (5164)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75685

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 50106800 - PDK2 NNNGCTGCCATCAAAGATGA NGG 0 1.0 Tier I
2 NC_000014.9 14 24102867 - PCK2 NNNGCGGCCACCAAAGATGA NGG 2 0.381 Tier I
3 NC_000008.11 8 95154320 + PLEKHF2 NNNGATTCCATCAAAGATGA NGG 2 0.3265 Tier I
4 NC_000008.11 8 25406960 - DOCK5 NNNACTGCCATCAAAAATGA NGG 2 0.9 Tier II
5 NC_000006.12 6 165386197 - PDE10A NNNGCTGCCATCAAAAATGG NGG 2 0.7647 Tier II
6 NC_000011.10 11 92543529 - FAT3 NNNGCTGCCATCAAAAATAA NGG 2 0.7143 Tier II
7 NC_000019.10 19 44061480 - ZNF223 NNNGCTGCCTTCAAAGATGG NGG 2 0.6747 Tier II
8 NC_000006.12 6 111726028 + FYN NNNGCTGCCTTTAAAGATGA NGG 2 0.4751 Tier II
9 NC_000006.12 6 37331207 - TBC1D22B NNNACTGCCATCAGAGATGA NGG 2 0.42 Tier II
10 NC_000001.11 1 6841106 - CAMTA1 NNNGCTGGCATCAAGGATGA NGG 2 0.4179 Tier II
11 NC_000020.11 20 61492917 + CDH4 NNNGCAGCCATGAAAGATGA NGG 2 0.3852 Tier II
12 NC_000014.9 14 24102867 - NRL NNNGCGGCCACCAAAGATGA NGG 2 0.381 Tier II
13 NC_000022.11 22 31443623 - EIF4ENIF1 NNNCCTGCCATCAAAGATGT NGG 2 0.3176 Tier II
14 NC_000017.11 17 33083688 + ASIC2 NNNGCTGCCATCAAAGAACA NGG 2 0.2989 Tier II
15 NC_000009.12 9 92749800 - BICD2 NNNGCTGCCATGGAAGATGA NGG 2 0.2899 Tier II
16 NC_000003.12 3 129512858 + IFT122 NNNGCTGCCATCAAAGAGGG NGG 2 0.2549 Tier II
17 NC_000001.11 1 115697531 + VANGL1 NNNGATGCCAGCAAAGATGA NGG 2 0.2286 Tier II
18 NC_000001.11 1 205271503 - TMCC2 NNNGCTGCCATCAAAGAGGT NGG 2 0.2 Tier II
19 NC_000001.11 1 25579532 + LDLRAP1 NNNGCTGCCATCAAAGATAA NAG 2 0.1852 Tier II
20 NC_000006.12 6 137146624 - IL22RA2 NNNGGTGCCATCTAAGATGA NGG 2 0.18 Tier II
21 NC_000002.12 2 197601001 - RFTN2 NNNGCTGCCCTCAGAGATGA NGG 2 0.1556 Tier II
22 NC_000012.12 12 121452888 - KDM2B NNNGCTGCCATTAAAGATGA NAG 2 0.1396 Tier II
23 NC_000009.12 9 83912328 - KIF27 NNNGCAGCCATCAAAGTTGA NGG 2 0.1156 Tier II
24 NC_000001.11 1 19731594 - TMCO4 NNNTCTGCCATCACAGATGA NGG 2 0.0779 Tier II
25 NC_000013.11 13 107335005 + NALF1 NNNGTTGCCATCAAAGATGA NGA 2 0.0442 Tier II
26 NC_000005.10 5 77348184 + PDE8B NNNGCTGCCATCACAGTTGA NGG 2 0.0286 Tier II
27 NC_000009.12 9 28511004 - LINGO2 NNNGCTGCCATCAAACATAA NGG 2 0.0 Tier II
28 NC_000001.11 1 6841106 - LOC124903832 NNNGCTGGCATCAAGGATGA NGG 2 0.4179 Tier III
29 NC_000013.11 13 20310960 - LOC105370102 NNNGCTGACATCAAAGATGA NAG 2 0.1685 Tier III
30 NC_000009.12 9 85819215 + LOC389765 NNNGCAGCCATCAAAGTTGA NGG 2 0.1156 Tier III
31 NC_000008.11 8 93560902 + CIBAR1-DT NNNGCTGCCATCAAAGAAGA NGA 2 0.0463 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 95030001 + Pdk2 NNNGCTGCCATCAAAGATGA NGG 0 1.0 Tier I
2 NC_000083.6 17 23945388 + Sbp NNNGCTGACAACAAAGATGA NGG 2 0.4875 Tier I
3 NC_000070.6 4 10991066 - Plekhf2 NNNGATTCCATCAAAGATGA NGG 2 0.3265 Tier I
4 NC_000079.6 13 15726571 + Gli3 NNNGATGCCATCATAGATGA NGG 2 0.3048 Tier I
5 NC_000077.6 11 69812456 - Gm39566 NNNGCTGCCATCCAAGATGC NGG 2 0.0478 Tier I
6 NC_000073.6 7 80625918 + Crtc3 NNNGCTACCATCAAAGAAGA NGG 2 0.6667 Tier II
7 NC_000083.6 17 43708773 - Cyp39a1 NNNGCAGCCAACAAAGATGA NGG 2 0.65 Tier II
8 NC_000075.6 9 67719922 - C2cd4b NNNGCTGTGATCAAAGATGA NGG 2 0.5417 Tier II
9 NC_000077.6 11 76967502 - Blmh NNNGATGTCATCAAAGATGA NGG 2 0.5 Tier II
10 NC_000071.6 5 127270377 - Tmem132c NNNGCTGCCAACAAAGAAGA NGG 2 0.5 Tier II
11 NC_000068.7 2 106228088 - Dcdc5 NNNGCTGCTATTAAAGATGA NGG 2 0.4712 Tier II
12 NC_000076.6 10 127725513 + Tac2 NNNGCTGGCACCAAAGATGA NGG 2 0.4286 Tier II
13 NC_000084.6 18 39094795 - Arhgap26 NNNGCTGACATCAAGGATGA NGG 2 0.4225 Tier II
14 NC_000073.6 7 78202925 + Ntrk3 NNNGCGGCCATCAAAGAAGA NGG 2 0.381 Tier II
15 NC_000076.6 10 14088334 - Hivep2 NNNGCTGCCATCAGAGATTA NGG 2 0.3111 Tier II
16 NC_000075.6 9 29664211 - Ntm NNNGATGCCATCAGAGATGA NGG 2 0.2667 Tier II
17 NC_000068.7 2 68459599 - Stk39 NNNGCTGTCATCAAAGATGC NGG 2 0.1989 Tier II
18 NC_000071.6 5 121273407 + Hectd4 NNNGCTGCCATGAAAGATGA NAG 2 0.1152 Tier II
19 NC_000067.6 1 133371514 + Etnk2 NNNGCTGCCATCAGAGGTGA NGG 2 0.0824 Tier II
20 NC_000080.6 14 65100425 + Extl3 NNNGCTGCCATCAAAGATGC NAG 2 0.0589 Tier II
21 NC_000071.6 5 8854753 + Abcb1b NNNGCTGCCATCAATGATGA NGA 2 0.0139 Tier II
22 NC_000076.6 10 90271393 + Anks1b NNNGATGCCATCAAAGATGA NGT 2 0.0092 Tier II
23 NC_000071.6 5 127270377 - Tmem132cos NNNGCTGCCAACAAAGAAGA NGG 2 0.5 Tier III
24 NC_000080.6 14 41148753 + Gm7920 NNNGATTCCATCAAAGATGA NGG 2 0.3265 Tier III
25 NC_000072.6 6 55791926 + Gm38820 NNNTCTGCCATCAAAGATGG NGG 2 0.2781 Tier III
26 NC_000070.6 4 53881750 + Gm12468 NNNCCTGCCATCAAAGATCA NGG 2 0.2373 Tier III
27 NC_000069.6 3 28203232 - Gm42196 NNNGCTGCCATGAGAGATGA NGG 2 0.2074 Tier III
28 NC_000077.6 11 103718498 + C130046K22Rik NNNGCTGCCGTCAAAGCTGA NGG 2 0.098 Tier III
29 NC_000080.6 14 65100425 + Gm52119 NNNGCTGCCATCAAAGATGC NAG 2 0.0589 Tier III
30 NC_000080.6 14 46487712 + Gm34756 NNNGCTGCCATGAAAGATGA NGA 2 0.0309 Tier III
31 NC_000078.6 12 7740571 - Gm32828 NNNGCTGCAATCAAAGATGA NGC 2 0.019 Tier III
32 NC_000070.6 4 48781663 - Gm52718 NNNGCTGCCCTCAAAGATGA NGT 2 0.0054 Tier III
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Other clones with same target sequence:

(none)