Construct: sgRNA BRDN0001145764

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGACATGGATAATCCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMTK2 (22853)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76174

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 98192203 + LMTK2 NNNACATGGATAATCCAGAA NGG 0 1.0 Tier I
2 NC_000016.10 16 28938904 + CD19 NNNACATGGATAATCCCGAT NGG 2 0.1059 Tier I
3 NC_000009.12 9 29173761 + LINGO2 NNNACATAGATAAACCAGAA NGG 2 0.619 Tier II
4 NC_000007.14 7 144007864 - OR6B1 NNNACTCGGATAATCCAGAA NGG 2 0.4911 Tier II
5 NC_000007.14 7 156812257 - LMBR1 NNNACCTGGATAATCCAAAA NGG 2 0.3147 Tier II
6 NC_000003.12 3 19995271 - PP2D1 NNNGCCTGGATAATCCAGAA NGG 2 0.2841 Tier II
7 NC_000020.11 20 47533413 + NCOA3 NNNACATGGAAAATCCAGAA NAG 2 0.1944 Tier II
8 NC_000013.11 13 93978019 - GPC6 NNNACTTGGATAATCCAGAA NAG 2 0.1852 Tier II
9 NC_000015.10 15 101486464 - PCSK6 NNNACATGGATAATCCAAAA NAG 2 0.1795 Tier II
10 NC_000015.10 15 101486464 - LOC124903566 NNNACATGGATAATCCAAAA NAG 2 0.1795 Tier II
11 NC_000005.10 5 75143237 + ANKRD31 NNNACCTGGATAATCCAGGA NGG 2 0.1705 Tier II
12 NC_000007.14 7 43727648 + COA1 NNNACATGAATAATCCAGAA NAG 2 0.1667 Tier II
13 NC_000002.12 2 70818095 - CLEC4F NNNACATGGATGCTCCAGAA NGG 2 0.152 Tier II
14 NC_000002.12 2 68718445 + ARHGAP25 NNNACTTGGATAATCCAGCA NGG 2 0.1478 Tier II
15 NC_000001.11 1 190342204 + BRINP3 NNNACCTGGATAATCCAGAA NAG 2 0.1178 Tier II
16 NC_000004.12 4 15712596 - BST1 NNNACATGGATAGTCCTGAA NGG 2 0.087 Tier II
17 NC_000005.10 5 147079542 + PPP2R2B NNNACATGGATAAACCTGAA NGG 2 0.0825 Tier II
18 NC_000023.11 X 37370134 - PRRG1 NNNACATGGATAAACCTGAA NGG 2 0.0825 Tier II
19 NC_000014.9 14 74591463 - LTBP2 NNNACATGGTTAATTCAGAA NGG 2 0.0588 Tier II
20 NC_000016.10 16 6078801 - RBFOX1 NNNACATGGAAAATCCAGAA NGA 2 0.0521 Tier II
21 NC_000005.10 5 36052439 - UGT3A2 NNNACATGGATAATGAAGAA NGG 2 0.05 Tier II
22 NC_000021.9 21 18022406 + CHODL NNNACTTGGATAATCCAGAA NGA 2 0.0496 Tier II
23 NC_000004.12 4 68082849 + TMPRSS11F NNNACAGGGATAATCCAGAA NGA 2 0.0408 Tier II
24 NC_000018.10 18 25183082 - ZNF521 NNNACATGGATAATGCATAA NGG 2 0.0333 Tier II
25 NC_000009.12 9 107318467 + RAD23B NNNACCTGGATAATCCAGAA NGA 2 0.0316 Tier II
26 NC_000010.11 10 88420647 + RNLS NNNACCTGGATAATCCAGAA NGA 2 0.0316 Tier II
27 NC_000009.12 9 14359301 - NFIB NNNACATGGATAATCCAGGA NGA 2 0.026 Tier II
28 NC_000001.11 1 27545561 - AHDC1 NNNTCATGGATAATCCAGAA NTG 2 0.0248 Tier II
29 NC_000015.10 15 47464039 + SEMA6D NNNACCTGGATAATGCAGAA NGG 2 0.0227 Tier II
30 NC_000014.9 14 44947475 + KLHL28 NNNACATGGATATTTCAGAA NGG 2 0.02 Tier II
31 NC_000007.14 7 155515096 + CNPY1 NNNACATGGATTATGCAGAA NGG 2 0.0167 Tier II
32 NC_000004.12 4 47554521 - ATP10D NNNAAATGGATAATCCAGAA NGC 2 0.0127 Tier II
33 NC_000015.10 15 50037707 - ATP8B4 NNNACATGGATAAGCCATAA NGG 2 0.0 Tier II
34 NC_000006.12 6 128933142 - LAMA2 NNNACATGGATAAGCCTGAA NGG 2 0.0 Tier II
35 NC_000008.11 8 62222631 + LOC124901952 NNNACATGGATAAACCAGAA NGG 1 0.619 Tier III
36 NC_000001.11 1 228961135 - LOC105373143 NNNACCAGGATAATCCAGAA NGG 2 0.3977 Tier III
37 NC_000003.12 3 592344 + LINC01266 NNNACATGGATAATAAAGAA NGG 2 0.2222 Tier III
38 NC_000019.10 19 31126032 + LOC105372356 NNNACCTGGATATTCCAGAA NGG 2 0.1364 Tier III
39 NC_000001.11 1 72568653 + LOC105378797 NNNACATGAATACTCCAGAA NGG 2 0.1353 Tier III
40 NC_000003.12 3 106560141 + LOC101929485 NNNAAATGGATAATCCAGAA NTG 2 0.0223 Tier III
41 NC_000012.12 12 12580029 + LOC107984486 NNNACCTGGATAATCCAGAA NGC 2 0.0101 Tier III
42 NC_000001.11 1 80573015 - LINC01781 NNNACATGGATAATCCAGAA NAA 2 0.0 Tier III
43 NC_000006.12 6 97314679 + LOC101927314 NNNACATGGATAATCCAGAA NAA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 10256058 - Myo16 NNNACATGGATAACCCAGAA NGG 1 0.2857 Tier II
2 NC_000082.6 16 27336009 + Ostn NNNAAATGGAGAATCCAGAA NGG 2 0.2286 Tier II
3 NC_000075.6 9 100601100 + Stag1 NNNACATGCAGAATCCAGAA NGG 2 0.2154 Tier II
4 NC_000072.6 6 47100346 + Cntnap2 NNNACATGGATAAACCAGAA NAG 2 0.1605 Tier II
5 NC_000068.7 2 72195220 + Rapgef4 NNNACATGTATAATCCAGAA NAG 2 0.1383 Tier II
6 NC_000069.6 3 135505545 + Manba NNNACATGGATAATCCAGGA NAG 2 0.0972 Tier II
7 NC_000072.6 6 101331269 + Pdzrn3 NNNACCTGGATACTCCAGAA NGG 2 0.0957 Tier II
8 NC_000070.6 4 83738941 - Ccdc171 NNNCCATGGATAATACAGAA NGG 2 0.0784 Tier II
9 NC_000086.7 X 69806841 + Aff2 NNNACATGGATAATGCAGAG NGG 2 0.0382 Tier II
10 NC_000068.7 2 157763741 - Ctnnbl1 NNNACATGGAAAATCCAGAA NTG 2 0.0292 Tier II
11 NC_000068.7 2 107316622 - Kcna4 NNNAAATGGATAATGCAGAA NGG 2 0.0286 Tier II
12 NC_000068.7 2 141084591 + Macrod2 NNNACATGGAGAATCCAGAA NGA 2 0.0278 Tier II
13 NC_000070.6 4 133068456 + Ahdc1 NNNTCATGGATAATCCAGAA NTG 2 0.0248 Tier II
14 NC_000070.6 4 107278197 + Hspb11 NNNACATGGATAAACCAGAA NTG 2 0.0241 Tier II
15 NC_000071.6 5 23757075 + Pus7 NNNACATGGATAATGCAGAA NTG 2 0.0019 Tier II
16 NC_000068.7 2 48536263 + Gm39809 NNNACAAGGATAATCAAGAA NGG 2 0.875 Tier III
17 NC_000077.6 11 117433862 + Gm11732 NNNACCTGTATAATCCAGAA NGG 2 0.2424 Tier III
18 NC_000070.6 4 94981922 + Gm12694 NNNACATGGAAAATACAGAA NGG 2 0.1667 Tier III
19 NC_000072.6 6 57501561 - 4930533I22Rik NNNACATGGATAAACCAGAA NAG 2 0.1605 Tier III
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Other clones with same target sequence:

(none)