Construct: sgRNA BRDN0001145766
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGACTTAAGCCGATCCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA7 (2045)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77352
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
93356760 |
- |
EPHA7 |
NNNACTTAAGCCGATCCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
43118054 |
+ |
HECW1 |
NNNACTTAAGCCGGTACCAG |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000002.12 |
2 |
124346123 |
+ |
CNTNAP5 |
NNNACTTAAGATGATCCCAG |
NGG |
2 |
0.4038 |
Tier II |
| 4 |
NC_000005.10 |
5 |
137178868 |
- |
SPOCK1 |
NNNTCTTAAGCCTATCCCAG |
NGG |
2 |
0.1909 |
Tier II |
| 5 |
NC_000007.14 |
7 |
43118054 |
+ |
HECW1-IT1 |
NNNACTTAAGCCGGTACCAG |
NGG |
2 |
0.4667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
28871954 |
+ |
Epha7 |
NNNACTTAAGCAGATCCCAG |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000072.6 |
6 |
63270990 |
+ |
Grid2 |
NNNAGTTAAGCAGATCCCAG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000079.6 |
13 |
63477235 |
+ |
Fancc |
NNNACTTAAACGGATCCCAG |
NGG |
2 |
0.4148 |
Tier II |
| 4 |
NC_000076.6 |
10 |
24285589 |
+ |
Moxd1 |
NNNACTTAAGCCTATCCCAG |
NGG |
1 |
0.3 |
Tier II |
| 5 |
NC_000081.6 |
15 |
81084053 |
+ |
Mrtfa |
NNNACGTAAGCCGATCACAG |
NGG |
2 |
0.2667 |
Tier II |
Other clones with same target sequence:
(none)
