Construct: sgRNA BRDN0001145767
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACTGTATTGTGTTGTCCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MET (4233)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76060
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
116757509 |
- |
MET |
NNNTGTATTGTGTTGTCCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
90747659 |
+ |
TTC7B |
NNNTATATTGTGTTGTCCCA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000002.12 |
2 |
153305179 |
+ |
GALNT13 |
NNNTGTATTGTGGTGTCCTG |
NGG |
2 |
0.1204 |
Tier II |
| 4 |
NC_000001.11 |
1 |
225327004 |
- |
DNAH14 |
NNNTGTATTGTGTTGTTGCG |
NGG |
2 |
0.0622 |
Tier II |
| 5 |
NC_000006.12 |
6 |
119614713 |
+ |
LOC105377975 |
NNNTCTATTGTGTTGTCCTG |
NGG |
2 |
0.3626 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
118448693 |
+ |
C1qtnf1 |
NNNTGTGCTGTGTTGTCCCG |
NGG |
2 |
0.7059 |
Tier II |
| 2 |
NC_000068.7 |
2 |
25279877 |
- |
Anapc2 |
NNNTGTGTTGTGTTGTCTCG |
NGG |
2 |
0.4538 |
Tier II |
| 3 |
NC_000076.6 |
10 |
86400705 |
+ |
Syn3 |
NNNTGTAGTGTGTTGTCCTG |
NGG |
2 |
0.3385 |
Tier II |
| 4 |
NC_000077.6 |
11 |
88439609 |
- |
Msi2 |
NNNTGTATTCTGTTGTCACG |
NGG |
2 |
0.2154 |
Tier II |
| 5 |
NC_000085.6 |
19 |
40336881 |
- |
Sorbs1 |
NNNTGTCTTGTGGTGTCCCG |
NGG |
2 |
0.1141 |
Tier II |
| 6 |
NC_000078.6 |
12 |
46766555 |
- |
Nova1 |
NNNTGTATTGTGTTTTGCCG |
NGG |
2 |
0.0084 |
Tier II |
| 7 |
NC_000077.6 |
11 |
118448693 |
+ |
Gm11747 |
NNNTGTGCTGTGTTGTCCCG |
NGG |
2 |
0.7059 |
Tier III |
| 8 |
NC_000073.6 |
7 |
138598809 |
- |
Gm30751 |
NNNTGCATTGTGTTGTCACG |
NGG |
2 |
0.4895 |
Tier III |
Other clones with same target sequence:
(none)
